Demonstration of a New Protein Fraction in Serum from the Human Fetus

Bergstrand, C. G.; Czar, B.

doi:10.3109/00365515609049266

Cited by 437 publications

(126 citation statements)

References 2 publications

Supporting

Mentioning

118

Contrasting

Unclassified

Order By: Relevance

“…The presence of alpha-fetoprotein (AFP) in human fetal serum was first reported by Bergstrand & Czar (1956). Subsequently, the same protein fraction was identified in a number of mammalian species (Gitlin & Boesman, 1967a).…”

Section: Introductionmentioning

confidence: 87%

Rat alpha-fetoprotein in experimental utero-placental ischaemia

Lai¹,

Hay²,

Forrester³

et al. 1976

Reproduction

View full text Add to dashboard Cite

Summary. Retardation of growth and death of fetal rats were produced after utero\x=req-\ placental ischaemia was induced by surgical ligation of the uterine arteries. Changes in maternal plasma levels of alpha-fetoprotein (AFP) were measured by radioimmunoassay. In rats in which the uterine blood supply was totally occluded, the resultant increase in maternal plasma AFP was due to resorption of fetal elements, because AFP levels in maternal rat plasma did not increase following hysterectomy in a control group. Maternal plasma AFP levels in rats with a partly occluded blood supply (and therefore some dead and some live fetuses) paralleled those of sham-operated rats, suggesting that increased placental transfer of AFP to maternal plasma may have offset the anticipated decline of AFP due to a decreased number of live fetuses.

show abstract

Section: Introductionmentioning

confidence: 87%

Rat alpha-fetoprotein in experimental utero-placental ischaemia

Lai¹,

Hay²,

Forrester³

et al. 1976

Reproduction

View full text Add to dashboard Cite

show abstract

“…1,2 It is produced in the fetus by the yolk sac and the fetal liver, and to a lesser extent also in the gastrointestinal tract. 3 AFP is a glycoprotein which contains up to 4% carbohydrates with a molecular mass varying from 66 to 70 kDa, depending on the carbohydrate content.…”

Section: Introductionmentioning

confidence: 99%

Alphafetoprotein in the Dutch External Quality Assurance programme: a need for improvement

2012

View full text Add to dashboard Cite

Background: Elevated alphafetoprotein (AFP) concentrations may result from a variety of clinical conditions, but their role as an important tumour marker has been well established. There may be differences in AFP values due to laboratories using different methods, even though most methods have been calibrated with the same international standard (WHO IS 72/225). Therefore it is important to know the analytical performance of the various methods in relation to the analytical requirements for AFP measurement. Methods: Annually, from January 2005 to July 2010, the results were analysed from the 65-75 laboratories that took part in the AFP survey of the External Quality Assurance programme of the Foundation Quality Control Medical Laboratories (the SKML/ Binding Analysis) in the Netherlands. Results: The Elecsys/Modular (36%) and the Immulite 2000/2500 (29%) are the methods used most. The methods show, on average, up to 15% positive and 12% negative bias, compared with the all-laboratory trimmed mean. Of the laboratories using the Immulite or the Elecsys/Modular analyser, over 70% show sufficient analytical performance to meet the Fraser criterion for method imprecision. Of the laboratories using a different method, over 50% do not meet this criterion. Conclusions: AFP immunoassays suffer from method bias, even though all methods have been calibrated with the same international standard. Some of the methods used show insufficient performance.

show abstract

“…1 Since the protein produced by the embryo at high levels is transferred to the maternal blood circulation, it is widely used in the clinic in order to detect fetal anomalies. Abnormal levels of embryonic AFP in the maternal serum are indicative of spina bifida or Down's syndrome in the fetus.…”

Section: Introductionmentioning

confidence: 99%

Congenital deficiency of alpha feto-protein

2004

View full text Add to dashboard Cite

Alpha-fetoprotein (AFP) is the main fetus serum glycoprotein with a very low concentration in the adult. AFP deficiency is a rare phenomenon. We studied two families with congenital AFP deficiency and searched for mutations in the AFP gene. We identified one mutation of 2 base deletion in exon 8, in both families, that leads to the congenital deficiency of AFP. The mutation nt930-931delCT (T294fs25X) creates a frameshift after codon 294 that leads to a stop codon after 24 amino acids, thus truncating the normal length of AFP of 609 amino acids. All the affected children were found to be homozygous for the mutation as was one of the fathers. The affected individuals were asymptomatic and presented normal development. This first identification of a mutation in the AFP gene demonstrates for the first time that deficiency of AFP is compatible with human normal fetal development and further reproduction in males.

show abstract

Demonstration of a New Protein Fraction in Serum from the Human Fetus

Cited by 437 publications

References 2 publications

Rat alpha-fetoprotein in experimental utero-placental ischaemia

Rat alpha-fetoprotein in experimental utero-placental ischaemia

Alphafetoprotein in the Dutch External Quality Assurance programme: a need for improvement

Congenital deficiency of alpha feto-protein

Contact Info

Product

Resources

About