Demonstration of a Surface Antigen of <i>Clostridium tyrobutyricum</i> by Use of Immunoblotting with a Monoclonal Antibody

Gueguen, Françoise; Robreau, Georges; Talbot, F.; Malcoste, R.

doi:10.1111/j.1348-0421.1990.tb00991.x

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“…Our first study showed the existence of a surface antigen with a ladder pattern closely similar in appearance to the lipopolysaccharide of Gram-negative bacteria when examined by SDS-PAGE. The inhibition of the antigen reactivity to a MoAb (AC2-41) after mild periodate oxidation sug-gests the involvement of a carbohydrate moiety in the epitope (16 twice with phosphate-buffered saline (PBS: 0.137 M NaCl; 2.7 X 10-3 M KO; 1.47 X 10-3 M K.H2PO4; 0.067 M Na2HPO4, 2H20) at pH 7.0. Antigen extraction (6).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Immunological Analyses of the Flagellin of Clostridium tyrobutyricum ATCC 25755

Arnold

Bédouet

Batina

et al. 1998

Microbiology and Immunology

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Section: Introductionmentioning

confidence: 99%

Biochemical and Immunological Analyses of the Flagellin of Clostridium tyrobutyricum ATCC 25755

Arnold

Bédouet

Batina

et al. 1998

Microbiology and Immunology

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Production of Monoclonal Antibodies Against the Outer Cell Wall ofClostridium tyrobutyricum

Talbot¹,

Robreau²,

Gueguen³

et al. 1994

Hybridoma

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Several hybridoma cell lines producing murine monoclonal antibodies (mAbs) directed to the Clostridium tyrobutyricum outer cell wall have been established and characterized. Whole bacteria, crude extract of cell wall, and polysaccharide fraction of crude extract have been used as immunogens. The immunizations were performed either in vivo or in vitro after priming in vivo. Amongst the clones obtained, six hybridoma cell lines were selected. Four mAbs recognized only the immunizing strain (ATCC 25755), while two mAbs recognized all the C. tyrobutyricum tested strains. Three mAbs were IgM, one IgG3, and two IgG1 isotypes. The antigens (proteins or polysaccharides) recognized by these mAbs have been characterized by Western Blot. These mAbs could be used for an early detection of C. tyrobutyricum in milk.

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Demonstration of a Surface Antigen of Clostridium tyrobutyricum by Use of Immunoblotting with a Monoclonal Antibody

Abstract: A monoclonal antibody,

Cited by 2 publications

References 21 publications

Biochemical and Immunological Analyses of the Flagellin of Clostridium tyrobutyricum ATCC 25755

Biochemical and Immunological Analyses of the Flagellin of Clostridium tyrobutyricum ATCC 25755

Production of Monoclonal Antibodies Against the Outer Cell Wall ofClostridium tyrobutyricum

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