Demonstration of acid phosphatase-containing vacuoles in hyphal tip cells of Sclerotium rolfsii

Hänssler, G.; Maxwell, D. P.; Maxwell, M. D.

doi:10.1128/jb.124.2.997-1006.1975

Cited by 29 publications

(6 citation statements)

References 19 publications

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“…Acid phosphatase (ACP) was measured by a modified method of Hänssler, Maxwell and Maxwell () with the assay kit of Jiancheng Bioengineering Institute (Nanjing, China). The release of p‐nitrophenol from p‐nitrophenyl phosphate in a reaction mixture incubated for 30 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary heat-killedLactobacillus plantarumL-137 (HK L-137) on the growth performance, digestive enzymes and selected non-specific immune responses in sea cucumber,Apostichopus japonicusSelenka

et al. 2015

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A feeding trial was conducted to evaluate the efficacy of dietary heat-killed Lactobacillus plantarum L-137 (HK L-137) on growth performance, digestive, non-specific immune and phagocytosis of sea cucumber, Apostichopus japonicus. Sea cucumbers (initial body weight 1.35 AE 0.04 g) were fed diets supplemented with five levels of HK L-137 (0, 0.005, 0.025, 0.05 and 0.25 g HK L-137 kg À1 diets) for 60 days. Results indicated sea cucumbers fed with diets containing 0.05 and 0.25 g HK L-137 kg À1 diets showed significantly (P < 0.05) higher body weight gain and specific growth rate than other groups. Sea cucumbers fed with diets containing 0.05 and 0.25 g HK L-137 kg À1 diets showed significantly (P < 0.05) higher protease activity than control group. Higher amylase, lysozyme and phagocytic activities were found in 0.25 g HK L-137 kg À1 diet group. Higher superoxide dismutase enzyme and alkaline phosphatase activity was found in 0.05 g HK L-137 kg À1 . While no significant differences (P > 0.05) were found in acid phosphatase activity. These results suggested that dietary supplementation of 0.05 g HK L-137 kg À1 diets would have benefit on growth, digestive enzymes and several non-specific immune parameters of sea cucumber.

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Section: Methodsmentioning

confidence: 99%

Effects of dietary heat-killedLactobacillus plantarumL-137 (HK L-137) on the growth performance, digestive enzymes and selected non-specific immune responses in sea cucumber,Apostichopus japonicusSelenka

et al. 2015

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“…Acid phosphatase (ACP) was measured by a modified method of Hänssler et al . () with the assay kit of Jiancheng Bioengineering Institute (Nanjing, China). The release of p‐nitrophenol from p‐nitrophenyl phosphate in a reaction mixture incubated for 30 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Optimum dietary protein level for growth and coelomic fluid non-specific immune enzymes of sea cucumberApostichopus japonicusjuvenile

Liao

Ren

et al. 2014

Aquacult Nutr

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A feeding trial was conducted to determine the optimum dietary protein level of sea cucumber Apostichopus japonicus juvenile focusing on growth performance and non‐specific immune response. Diets with seven crude protein levels (42.0, 108.9, 155.2, 216.7, 258.0, 313.3 and 357.5 g kg−1) were fed to sea cucumber juveniles (1.05 ±0.01 g) once a day for 100 days. More than 70% survival was observed, and there was no significant difference among all treatments. The sea cucumbers fed diets containing 108.9 g kg−1 crude protein showed significantly (P < 0.05) higher body weight gain than those of the sea cucumbers fed diets containing 42.0, 216.7, 258.0, 313.3 and 357.5 g kg−1 crude protein. No significantly differences (P > 0.05) were observed in moisture, crude protein, crude lipid, ash and carbohydrate content of the body wall among all treatments. The coelomic fluid catalase activity of the sea cucumbers generally increased with increasing dietary protein levels. Therefore, the acid phosphatase, superoxide dismutase and lysozyme activity increased with increasing dietary protein levels at first and decreased subsequently. The relationship between dietary protein levels and body weight gain was analysed by a second‐order polynomial regression analysis model. The result indicates that the optimum dietary protein level for sea cucumber juveniles is 135.4 g kg−1.

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“…The difference in vacuolization between cells grown at pH 6.0 and 7.0 tempts one to speculate that the large, water-filled vacuoles seen in cells grown at pH 6.0 may also contain all or part of the stored GOase. Other enzymes such as acid phosphatase from Sclerotium rolfsii (15) and cellulase from Ochyla ambisexualis (20) are localized in the vacuole portion of the cell. It is known that vacuoles can form by pinching off from the endoplasmic reticulum, thereby encapsulating recently synthesized protein (14).…”

mentioning

confidence: 99%

Regulation of galactose oxidase synthesis and secretion in Dactylium dendroides: effects of pH and culture density

Shatzman¹,

Kosman²

1977

J Bacteriol

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The effects of pH and growth density on the amount of an extracellular enzyme, galactose oxidase, synthesized by the fungus Dactylium dendroides were studied. Growth at a pH below 6.7 caused a decrease in the ability of the organism to release galactose oxidase. The enzyme retained by these fungal cells was liberated whenever the pH was raised to 7.0. Cycloheximide addition failed to inhibit the appearance of this protein; [3H]leucine added prior to pH adjustment was not incorporated into the released protein. These observations indicate the released protein is not newly synthesized protein. The retained enzyme would be secreted slowly over a 2-day period if the pH was not increased. In addition to regulating protein retention, pH was also shown to be associated with vacuolization, cell volume, culture density, and inhibition of protein synthesis. Cultures maintained at low pH were characterized by a dense growth consisting of highly vacuolated, buoyant, fungal hyphae. Increasing the pH from 6 to 7 caused a decrease in vacuole size. Cells grown at neutral pH maintained a lower density of growth and, based on activity measurements, synthesized 33% more galactose oxidase. Furthermore, cultures grown at pH 6.0 and maintained at a lower cell density produced galactose oxidase at a level similar to that of cells grown at neutral pH. Thus, the elevated density of the cell culture was inhibitory to galactose oxidase synthesis. The observed effects on protein synthesis and release were rather specific for galactose oxidase, since other extracellular proteins appeared in the earliest stages of growth. celium as seen by phase-contrast microscopy. Volume increases and decreases are associated with 455 on August 3, 2020 by guest

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Demonstration of acid phosphatase-containing vacuoles in hyphal tip cells of Sclerotium rolfsii

Cited by 29 publications

References 19 publications

Effects of dietary heat-killedLactobacillus plantarumL-137 (HK L-137) on the growth performance, digestive enzymes and selected non-specific immune responses in sea cucumber,Apostichopus japonicusSelenka

Effects of dietary heat-killedLactobacillus plantarumL-137 (HK L-137) on the growth performance, digestive enzymes and selected non-specific immune responses in sea cucumber,Apostichopus japonicusSelenka

Optimum dietary protein level for growth and coelomic fluid non-specific immune enzymes of sea cucumberApostichopus japonicusjuvenile

Regulation of galactose oxidase synthesis and secretion in Dactylium dendroides: effects of pH and culture density

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