Demonstration of aldosterone and corticosterone as the principal steroids formed in incubates of adrenals of the American bullfrog (Rana catesbeiana) and stimulation of their production by mammalian adrenocorticotropin

Carstensen, Helena; Burgers, A. C. J.; Li, Choh Hao

doi:10.1016/0016-6480(61)90023-5

Cited by 119 publications

(16 citation statements)

References 29 publications

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“…Specific effects of ACTH on the secretion of electrolyte-active hormone were suggested by the augmentation of skin short circuit current in ACTHtreated frogs (6). In vitro stimulation of aldosterone synthesis in bullfrog interrenal incubates by the addition of mammalian ACTH and of frog pituitary extract was shown by Carstensen, Burgers, and Li (2). The present data confirm stimulation of aldosterone secretion in vivo by mammalian ACTH.…”

Section: Discussionsupporting

confidence: 79%

Metabolism and rate of secretion of aldosterone in the bullfrog

Ulick¹,

Feinholtz²

1968

J. Clin. Invest.

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A study of the metabolism of aldosterone in the bullfrog was undertaken to provide a measurable metabolite for the indirect isotope dilution technique for measuring secretory rates. The rate of excretion of labeled aldosterone was considerably slower in the frog than in man and made necessary the collection of excretory products for 5 days to insure reasonably complete recovery of metabolites. The major identifiable metabolite was a tetrahydro derivative subsequently identified as 3,f-hydroxy-5,8-tetrahydroaldosterone. This metabolite was excreted partly in the free form and partly as a glucuronic acid conjugate. The pH 1-hydrolyzable conjugate of aldosterone was not detected.For the measurement of secretory rates, aldosterone-3H was injected into the dorsal lymph space and the animal placed in a bath to provide an environment of constant electrolyte composition and intake and a means of collecting excretory products. Urine and bath fluid were collected for 5 days, tetrahydroaldosterone was isolated, and its specific activity determined for the calculation of the aldosterone secretory rate. The rate of secretion of aldosterone in the bullfrog was increased by a tap water bath and by bovine adrenocorticotropic hormone, decreased by a saline bath and by dexamethasone, and unchanged by valine-5-angiotensin amide.

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Section: Discussionsupporting

confidence: 79%

Metabolism and rate of secretion of aldosterone in the bullfrog

Ulick¹,

Feinholtz²

1968

J. Clin. Invest.

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“…It would appear, therefore, that the biosynthetic pathways are similar in lower vertebrates and mammals. Moreover, amphenone which is known to reduce 11/3, 17« and 21 hydroxylations and oxidation of the A5-3-hydroxy group to A4-3-ketone in mammals (Rosenfeld & Bascom, 1956;Renold, Crabbé, HernandoAvendano, Nelson, Ross, Emerson & Thorn, 1957; Chart, Sheppard, Allen, Bencze & Gaunt, 1958) also reduced the formation of aldosterone and corticosterone in reptiles. Thus it seems likely that lower vertebrates share with mammals similar adrenocortical enzyme systems.…”

Section: Discussionmentioning

confidence: 95%

“…Model LP2A). Measurements of absolute amounts were determined after acetylation by ultraviolet spectrophotometry (Unicam, SP.500) using the formula 240 = 2 ,4 240-(.4 230+ .4 250) where 240 denotes the corrected absorbancy and A denotes absorbancy at the indicated wavelength (Allen, 1950;Carstensen et al 1961).…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis of Adrenocortical Hormones by Adrenal Glands of Lizards and Snakes

Jg¹,

Ic²,

Bellamy³

1962

Journal of Endocrinology

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“…Carstensen et al (92) reported that the adrenal of the American bullfrog produces mainly aldosterone and corticosterone, the ratio of aldosterone to corticosterone was 4:1, and ACTH stimulated the production of aldosterone much more than that of corticosterone. Kraulis and Birmingham (93) reported the conversion, in frog adrenals, of 11 [3-hydroxyprogesterone to corticosterone ru1d to fractions with the mobility of aldosterone and 18-hydroxycorticosterone.…”

Section: Corticoid Secretion In Amphibiansmentioning

confidence: 99%