Demonstration of .ALPHA.1-adrenoceptors in human hypertrophied prostate with tritiated bunazosin.

Moriyama, Nobuo; Kawabe, Kazuki; Hamada, Kozo; Kimura, Kiyoshi; Murahashi, Isao; Aso, Yoshio

doi:10.1267/ahc.24.421

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…Prostatic tissue is mainly composed of glandular acini and stroma, the latter consisting mainly of smooth muscle cells, fibroblasts and connective tissue. Prostatic contraction is mediated by the stimulation of receptors on the surface of smooth muscle cells; α1‐adrenoceptors, which play a major role in prostatic contraction [ 11], are also known to be localized on smooth muscle cells and not on glandular cells [ 4–7]. Therefore, it is important to determine the proportion of smooth muscle area in each histological compartment of the prostate.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of histological structure and its effect on the distribution of α1‐adrenoceptors in human benign prostatic hyperplasia

Kurimoto

Moriyama

Hamada

et al. 1998

British Journal of Urology

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Objective To evaluate the histological structure of benign prostatic hyperplasia (BPH) and its relationship with the density of α1‐adrenoceptors in smooth muscle. Materials and methods Specimens from hyperplastic tissues obtained from 14 patients with BPH were evaluated for the density of α1‐adrenoceptors in smooth muscle using autoradiography, Mallory‐Azan staining and computer‐assisted image analyses. The binding of [3H] tamsulosin (a selective α1‐blocker) and the ratio of smooth muscle area was calculated, and the density of α1‐adrenoceptors per area of smooth muscle determined by dividing the degree of binding by the ratio of smooth muscle to total area. Results There was a significant difference between the ratio of smooth muscle area in the hyperplastic acinar nodule and the surrounding stroma (P<0.01). The density of α1‐adrenoceptor per smooth muscle area was significantly higher in the hyperplastic acinar nodule than in the surrounding stroma (P<0.05). There was no correlation between prostatic weight and the ratio of smooth muscle area or the density of α1‐adrenoceptors in each region. Conclusion The distribution of α1‐adrenoceptors on smooth muscle differed with histological structure; both the histological conformation and the difference in the distribution of α1‐adrenoceptors could affect urethral obstruction in BPH.

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Section: Discussionmentioning

confidence: 99%

“…Its role and distribution have been evaluated by contractile studies, binding assays and autoradiography. Several autoradiographic studies have characterized the population of α1‐adrenoceptors on smooth muscle cells and their absence on glandular cells [ 3–7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of histological structure and its effect on the distribution of α1‐adrenoceptors in human benign prostatic hyperplasia

Kurimoto

Moriyama

Hamada

et al. 1998

British Journal of Urology

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“…Using isotope-labelled o~ blockers, many experiments have been performed in several organs, such as brain (Morrow & Cresse, 1986;Tsuchihashi et al, 1991), blood vessels (Maruyama et al, 1993), kidney (Feng et al, 1993) and prostate (Kawabe et al, 1990a;Moriyama et al, 1991Moriyama et al, , 1994. However, studies as to the distribution of o~ 1 adrenoceptors in human prostate are rare because of the limitations of tissue samphng or methods of quantification.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro autoradiography The method of in vitro macroautoradiography has been described elsewhere (Moriyama et al, 1991(Moriyama et al, , 1994. In brief, specimens were cut into 10/~m-thick sections and mounted on poly-L-lysine coated slide glasses.…”

Section: (A)mentioning

confidence: 99%

“…More-0018-2214 9 1995 Chapman & Hall over, their evaluation was limited by the sampling of specimens or the method of quantification. We attempted autoradiographic visualization of 0/1 adrenoceptors in whole prostatic sections using a computerized image analysis system (Kawabe et al, 1990a;Moriyama et al, 1991Moriyama et al, , 1994. Applying this technique, we further studied the quantification of 0/1 adrenoceptors in several zones (urethral, glandular and stromal) in the hypertrophied prostatic tissues.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative autoradiography of α1 adrenoceptors with [3H]tamsulosin in human hypertrophied prostate using computerized image analysis

Kurimoto¹,

Moriyama²,

Hamada³

et al. 1995

Histochem J

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Fourteen specimens of human hypertrophied prostate were evaluated for the distribution of alpha 1 adrenoceptors using autoradiography with a computerized image analysis system. The hypertrophied prostatic specimens, obtained at open prostatectomy, were dissected vertically to the urethra, and sectioned at 10 microns. They were immersed in 1 nM of specific alpha 1 ligand, [3H]tamsulosin chloride ([3H]tamsulosin) and exposed to autoradiographic film. The images were analysed by a computerized image analysis system. The total binding of [3H]tamsulosin in the whole section (n = 14) was 0.82 +/- 0.21 (mean +/- SE) nCi mg-1. The autographic data were correlated with data obtained in a membrane-binding assay. The prostatic tissue studied was divided into urethral, glandular and stromal zones, the latter two zones being further divided into the inner and outer areas. The total binding of [3H]tamsulosin in the urethral zone (n = 7) was 0.65 +/- 0.32 nCi mg-1. The glandular zone contained significantly more abundant alpha 1 adrenoceptors than the stromal zone and their densities (glandular vs stromal) were 1.15 +/- 0.19 nCi mg-1 (n = 14) vs 0.72 +/- 0.15 nCi mg-1 (n = 14), respectively (p < 0.05). The data from the whole section were not affected by prostatic weight. This method described enabled the distribution of the receptors in different sites to be evaluated both morphologically and quantitatively.

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Age-related changes in alpha1-adrenoceptors in rat prostate

et al. 1995

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The age-related changes in the density of alpha 1-adrenoceptors in the dorsolateral lobe of the rat prostate were evaluated in Wistar rats at 8, 52, and 104 weeks of age. [3H]YM617, a newly synthesized alpha 1-adrenergic blocker, was used as the ligand. The mean maximum number of binding sites, or alpha 1-adrenoceptor density (Bmax) +/- SE of 104-week-old rats (11.0 +/- 1.2 fmol/mg protein) was significantly lower than that in the 8-week-old rats (37.0 +/- 4.3 fmol/mg protein) and 52-week-old rats (37.2 +/- 3.4 fmol/mg protein) (respectively, P < 0.01). In contrast, mean affinity (Kd) values +/- SE of these groups showed no significant differences (8-week-old rats, 115.8 +/- 9.1; 52-week-old rats, 100.5 +/- 5.8; and 104-week-old rats, 116.4 +/- 9.8 pM). Mean volumes +/- SE of muscle cells of the prostate were 3.7 +/- 1.1 x 10(3) microns3 at 8 weeks, 30.0 +/- 6.2 x 10(3) microns3 at 52 weeks, and 18.6 +/- 8.2 x 10(3) microns3 at 104 weeks. Volumes for 8-week-old rats were significantly smaller than those for 52-week-old (P < 0.01) and 104-week-old rats (P < 0.05). However, the mean area density of the muscle cells showed no difference among the three groups: 20.1 +/- 2.2% at 8 weeks, 27.3 +/- 2.9% at 52 weeks and 20.3 +/- 3.4% at 104 weeks. In conclusion, the density of YM617-binding sites (alpha 1-adrenoceptors) in 104-week-old rats was lower than in 8- and 52-week-old rats.(ABSTRACT TRUNCATED AT 250 WORDS)

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Demonstration of .ALPHA.1-adrenoceptors in human hypertrophied prostate with tritiated bunazosin.

Cited by 10 publications

References 12 publications

Evaluation of histological structure and its effect on the distribution of α1‐adrenoceptors in human benign prostatic hyperplasia

Evaluation of histological structure and its effect on the distribution of α1‐adrenoceptors in human benign prostatic hyperplasia

Quantitative autoradiography of α1 adrenoceptors with [3H]tamsulosin in human hypertrophied prostate using computerized image analysis

Age-related changes in alpha1-adrenoceptors in rat prostate

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