Demonstration of Auxin Binding to Strawberry Fruit Membranes

Narayanan, K. R.; Mudge, Kenneth W.; Poovaiah, B. W.

doi:10.1104/pp.68.6.1289

Cited by 19 publications

(9 citation statements)

References 16 publications

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“…Although the mechanism of this latter IAA aetion is not yet understood, there is some evidence that IAA acts along the pathway of phloem transport and enhanees transport rates to the sites of high IAA eontent (Patrick 1979). In agreement with this, IAA enhanees the growth rate of strawberry fruits, for example (Narayanan et al 1981). In these experiments, application of IAA was quite effective in stimulating the growth rate of storage tissue.…”

Section: Discussionsupporting

confidence: 79%

Growth rate of potato tubers and endogenous contents of indolylacetic acid and abscisic acid

Marschner

Sattelmacher

Bangerth

1984

Physiologia Plantarum

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1984. Growth rate of potato tubers and endogenous eontents of indolylaeetic acid and abscisic acid. -Physiol. Plant. fiO: 16-20.Potato plants {Solanum luhcrosum L. cv. Ostara) were grown in waler culture and the growth rate of individual tubers was measured daily or at two day intervals. Tubers of different growth rate and/or different age (days after tuberization) were harvested and analysed for indolylaeetic acid (IAA) and abseisic acid (ABA). Within individual tubers tbe IAA content decreases from the apieal to the basal part of the tuber. Tuber age and eorresponding fresh weight are negatively correlated with the endogenous IAA content. If, however, individual tubers of comparable age but different growth rates are compared, a significant positive correlation between growth rate and IAA content is revealed, while ABA showed a significant negative eorrelation with growth rate. Removal of all fast-growing tubers from individual plants causes an increase in the growth rate of the remaining tubers within 3-1 days. This coincides with a particularly steep increase in IAA content. The data suppjjrt the idea that endogenous IAA content may be one factor responsible for controlling the growth rate (•"sink-activity") of individual tubers.Additional key words -Distribution of IAA, Solatium lubcrosnm H. Marschner Baiifierih, Univ. Hohvnheim,

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Section: Discussionsupporting

confidence: 79%

Growth rate of potato tubers and endogenous contents of indolylacetic acid and abscisic acid

Marschner

Sattelmacher

Bangerth

1984

Physiologia Plantarum

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“…The level of IAA in dormant buds is unchanged from that in stratified buds after rest; that is, the amount if IAA is apparently not involved in the shifting hormone balances that are thought to be an important ingredient for breaking winter dormancy (Wareing and Saunders 1971). The total molar IAA concentrations during the growth flush can be estimated, assuming a 30 percent dry matter in elongating tissue, to be approximately 0.5 J..LM, a magnitude in reasonable agreement with the exogenously applied concentration of IAA which successfully stimulates rapid growth in other plant tissue (Penny and Penny 1978).…”

Section: Discussionsupporting

confidence: 55%

“…Its physiological action has been under study for some time. It has a greater affinity to corn-and strawberryderived receptors than IAA and thus displaces IAA as ligand in this in vitro system (Ray, Dohrmann, and Hertel 1977;Narayanan, Mudge, and Poovaiah 1981). In our work we found that adding small amounts of dissolved NAA to suspensions of crystalline IAA-Kin chloroform or 20 percent benzene in petroleum ether increased the solubility of IAA in these nonpolar solvents (table 4).…”

Section: Extraction Mediamentioning

confidence: 50%

Methodology for indole-3-acetic acid: Sample preparation, extraction, and purification techniques

Martin¹,

Kammereck²,

Nishijima³

1986

Hilg

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Indole-3-acetic acid (IAA) has been implicated as a regulating agent in numerous plant-growth phenomena. In an attempt to assess its role in plants, identification and quantification are necessary. Estimating the amount of IAA in plant samples is difficult because this molecule degrades rapidly during most extraction-purification systems. A method is presented which reduces IAA degradation to a minimum by drying the sample at about 10-5 torr of oxygen and subsequently extracting with anhydrous solvent. A review of earlier methods is included.

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“…8). Narayanan et al (18,19) reported that the optimum pH for auxin binding of a plasma membrane-enriched fraction was well below pH 4.5. Thus, the optimum pH for auxin per se or of structural analogs such as NPA may be variable, depending on the plant materials. In the present study, however, the NPA binding assay was performed at pH 5 to prevent the deleterious effect of a low pH on membranes, especially on endomembranes which were found to be highly sensitive to pH values below 5.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Plasma Membrane from Light-Grown Winter Rye Seedlings (Secale cereale L. cv Puma)

Uemura

Yoshida²

1983

Plant Physiol.

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An effective method for the isolation of plasma membrane from lightgrown winter rye seedlings (Secale cereak L. cv Puma) was established using a liquid two-polymer phase separation. The conditions for the specific partition of plasma membrane into the polyethylene glycolenriched upper phase were examine, including variations in the polymer concentration, buffer system, pH, and NaCl addition in the phase partition system. The most effective phase partition system for the isolation of plasma membrane from winter rye consisted of 5.6/5.6% (w/w) polyethylene glycol 4000/dextran T500 in 0.25 molar sucrose-10 millimolar potassium phosphate-30 millimolar NaCI (pH 7.8), repeated once. When the isolated plasma membrane was centrifuged on a linear sucrose density gradient, a single band was found at the 34% (w/w) sucrose layer (1.141 grams per cubic centimeter) which co-fractionated with the pH 6.5-ATPase.Identification of plasma membrane was performed by the combination of phosphotungstic acid-chromic acid stain and specific binding of N-1-naphthylphthaamic acid. Based on morphometrical observations after phosphotungstic acid-chromic acid stain, the isolated plasma membrane consisted mostly ofvesicles of high purity. The isolated plasma membrane also showed extremely high specificity for N-1-naphthylphthalamic acidbindin, 10-fold higher than other membranes. It was also confirmed that there is a distinct difference in properties between plasma membrane and other membranes. The endomembranes such as from chloroplasts, mitochondria, and endoplasmic reticulum were observed to be highly sensitive to Zn2" ion and lower pH, which resulted in an abrupt aggregation of membranes. On the contrary, plasma membrane was very stable to these treatments and no aggregation was observed. These unique properties of isolated plasma membrane are generally observed in a wide variety of plant species and can be utilized for the assessment of the purity of preparations of isolated plasma membranes and for their identification. process.A major impediment to these approaches, however, has been in the difficulties of isolating plasma membranes in both a sufficiently pure form and a state identical with their in vivo state in plants. In most cases, plasma membrane-enriched fractions have been isolated mainly from tender and non-photosynthesizing tissues such as roots (3,12, 28), etiolated seedlings (15, 21), and protoplasts (5,9,20) by the combination of mechanical disruption and sucrose density gradient centrifugations. One of the major problems encountered by investigators attempting to isolate plasma membranes from differentiated green tissues with thick cell walls has been the cross-contamination of fragmented chloroplast membranes, which are very difficult to separate from other membranes by differences in density or sedimentation characteristics. Furthermore, the sucrose density gradient centrifugations are lengthy, and the medium per se creates an osmotic gradient which may affect, to some extent, the intactness of the isolated memb...

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Demonstration of Auxin Binding to Strawberry Fruit Membranes

Cited by 19 publications

References 16 publications

Growth rate of potato tubers and endogenous contents of indolylacetic acid and abscisic acid

Growth rate of potato tubers and endogenous contents of indolylacetic acid and abscisic acid

Methodology for indole-3-acetic acid: Sample preparation, extraction, and purification techniques

Isolation and Identification of Plasma Membrane from Light-Grown Winter Rye Seedlings (Secale cereale L. cv Puma)

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