Demonstration of Charcot-Leyden Crystals in Eosinophilic Cystitis

Staribratova, D; Belovejdov, V.; Staikov, Dimitri; Dikov, Dorian

doi:10.5858/2009-0604-le.1

Cited by 11 publications

(4 citation statements)

References 3 publications

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“…Notably, however, H&E staining has limited power for the identification of Charcot-Leyden crystals, especially with the presence of apoptotic and necrotic cells in the background. 22 MPP is considered refractory if clinical and radiological deterioration occurs despite appropriate antibiotic therapy for !7 days. 11 We found that significantly more patients in the BC than non-BC group had refractory MPP.…”

Section: Discussionmentioning

confidence: 99%

Bronchial casts associated with Mycoplasma pneumoniae pneumonia in children

Liu

Cai

et al. 2020

J Int Med Res

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This study was performed to analyze 22 cases of Mycoplasma pneumoniae pneumonia (MPP) associated with bronchial casts (BCs) in children. Methods: We retrospectively reviewed all cases of MPP in children treated at our institution from November 2015 to December 2016. Demographic information, laboratory parameters, radiologic and fiberoptic bronchoscopy findings, treatment outcomes, and follow-up results were analyzed. Results: Among 161 patients with MPP, 22 had BCs and 139 had no BCs. All BCs occurred in a segmental or subsegmental bronchus and were removed by fiberoptic bronchoscopy. Patients with BCs had a longer duration of fever after admission and higher incidence of refractory MPP. Substantially more children with than without BCs had a high M. pneumoniae load in the bronchoalveolar lavage fluid. All patients with BCs but only 55.4% without BCs were given methylprednisolone in addition to the standard antibiotic treatment. A significantly higher proportion of children with than without BCs received oxygen therapy. After discharge, complete radiological resolution took significantly longer in children with than without BCs. Conclusions: In children with MPP, prompt removal of BCs may be necessary to prevent BC propagation. MPP with BCs is more severe than that without BCs, and treatment and recovery are more difficult.

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Section: Discussionmentioning

confidence: 99%

Bronchial casts associated with Mycoplasma pneumoniae pneumonia in children

Liu

Cai

et al. 2020

J Int Med Res

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“…Charcot–Leyden crystals (CLCs) have been described as extracellular deposits of morphologically diverse crystals in inflamed tissues of patients [ 1 , 2 ], especially in eosinophils, macrophages, and basophils in the sputa of patients with bronchial asthma [ 3 ]. CLCs are considered hallmarks of eosinophil involvement in many diseases, such as allergic rhinitis [ 4 ], eosinophilic cystitis [ 5 ], atopic dermatitis [ 6 ], celiac disease [ 7 ], asthma [ 8 ], acute myeloid leukemia [ 9 ], colorectal cancer [ 10 ], mastocytoma [ 11 ], periapical lesion [ 12 ], and parasitic infections in the liver [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Lactose and Galactose Promote the Crystallization of Human Galectin-10

Jiang

Zhang

et al. 2023

Molecules

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Galectin-10 (Gal-10) forms Charcot–Leyden crystals (CLCs), which play a key role in the symptoms of asthma and allergies and some other diseases. Gal-10 has a carbohydrate-binding site; however, neither the Gal-10 dimer nor the CLCs can bind sugars. To investigate the monomer–dimer equilibrium of Gal-10, high-performance size-exclusion chromatography (SEC) was employed to separate serial dilutions of Gal-10 with and without carbohydrates. We found that both the dimerization and crystallization of Gal-10 were promoted by lactose/galactose binding. A peak position shift for the monomer was observed after treatment with either lactose or galactose, implying that the polarity of the monomer was reduced by lactose/galactose binding. Further experiments indicated that alkaline conditions of pH 8.8 mimicked the lactose/galactose-binding environment, and the time interval between monomers and dimers in the chromatogram decreased from 0.8 min to 0.4 min. Subsequently, the electrostatic potential of the Gal-10 monomers was computed. After lactose/galactose binding, the top side of the monomer shifted from negatively charged to electrically neutral, allowing it to interact with the carbohydrate-binding site of the opposing subunit during dimerization. Since lactose/galactose promotes the crystallization of Gal-10, our findings implied that dairy-free diets (free of lactose/galactose) might be beneficial to patients with CLC-related diseases.

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“…Gal-10 is overexpressed in eosinophils [7] and can spontaneously form CLCs within these cells [8] or upon eosinophil lysis [9] . CLCs are frequently observed in eosinophil-based diseases, such as acute myeloid leukemia, mastocytoma [10] , allergic rhinitis [11] , celiac disease [12] , and eosinophilic cystitis [13] . Recently, a humanized mouse model was used to demonstrate that CLCs directly induce asthma [14] .…”

Section: Introductionmentioning

confidence: 99%

Glutathione disrupts galectin-10 Charcot-Leyden crystal formation to possibly ameliorate eosinophil-based diseases such as asthma

Na¹,

Sayed²,

Ayala³

et al. 2023

ABBS

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Charcot-Leyden crystals (CLCs) are the hallmark of many eosinophilic-based diseases, such as asthma. Here, we report that reduced glutathione (GSH) disrupts CLCs and inhibits crystallization of human galectin-10 (Gal-10). GSH has no effect on CLCs from monkeys ( Macaca fascicularis or M. mulatta ), even though monkey Gal-10s contain Cys29 and Cys32. Interestingly, human Gal-10 contains another cysteine residue (Cys57). Because GSH cannot disrupt CLCs formed by the human Gal-10 variant C57A or inhibit its crystallization, the effects of GSH on human Gal-10 or CLCs most likely occur by chemical modification of Cys57. We further report the crystal structures of Gal-10 from M . fascicularis and M . mulatta , along with their ability to bind to lactose and inhibit erythrocyte agglutination. Structural comparison with human Gal-10 shows that Cys57 and Gln75 within the ligand binding site are responsible for the loss of lactose binding. Pull-down experiments and mass spectrometry show that human Gal-10 interacts with tubulin α-1B, with GSH, GTP and Mg 2+ stabilizing this interaction and colchicine inhibiting it. Overall, this study enhances our understanding of Gal-10 function and CLC formation and suggests that GSH may be used as a pharmaceutical agent to ameliorate CLC-induced diseases.

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Demonstration of Charcot-Leyden Crystals in Eosinophilic Cystitis

Cited by 11 publications

References 3 publications

Bronchial casts associated with Mycoplasma pneumoniae pneumonia in children

Bronchial casts associated with Mycoplasma pneumoniae pneumonia in children

Lactose and Galactose Promote the Crystallization of Human Galectin-10

Glutathione disrupts galectin-10 Charcot-Leyden crystal formation to possibly ameliorate eosinophil-based diseases such as asthma

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