Demonstration of Delayed Hypersensitivity in Chlamydia trachomatis Salpingitis in Monkeys: A Pathogenic Mechanism of Tubal Damage

Patton, Dorothy L.; Sweeney, Yvonne T. Cosgrove; Kuo, Cho‐Chou

doi:10.1093/infdis/169.3.680

Cited by 97 publications

(56 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, both methods are costly and invasive, and therefore unsuitable for screening on a large scale. Since tubal pathology and infertility have been associated with asymptomatic Chlamydia trachomatis infections in the past, chlamydia antibody testing (CAT) in serum has been introduced as a screening method for tubal factor subfertility [7][8][9][10]. Hence the development of a simple and reliable assay for the detection of C. trachomatis antibodies is essential.…”

Section: Introductionmentioning

confidence: 99%

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Muvunyi

Claeys

Sutter

et al. 2011

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility, using laparoscopy as a reference. Methodology: Four commercial serological methods were used to analyse 101 serum samples for the presence of C. trachomatis IgG antibodies from patients at the Infertility Clinic of Ghent University Hospital. The diagnostic utility for prediction of tubal infertility of serological methods was evaluated based on patients' medical records. Results: A comparison of the serological assays showed little difference in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100% for all assays (except the ELISA Vircell, with a sensitivity of 90%), and the specificities ranged from 92% for MIF Ani Labsystems to 98% for the MIF Focus and ELISA Vircell. As compared to laparoscopy data, CAT positivity in subfertile women with tubal damage (n=40) did not significantly differ from that of subfertile women without tubal damage (n=61): Positive predictive values (PPV) of CAT ranged from 53% to 60% and negative predictive values (NPV) ranged from 62% to 64%. Conclusion: evaluated ELISAs are comparable to MIFs in the detection of C. trachomatis IgG antibodies and should be preferred for large serological studies, especially in resource poor settings.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Muvunyi

Claeys

Sutter

et al. 2011

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Later Morrison and coauthors [36,37] demonstrated that a 57-kDa protein, identified as chlamydial HSP60, is the active component and responsible for the inflammatory response in trachoma. Experimental studies in the monkey`pocket' model of salpingitis have further supported the role of HSP60-specific T cells in the pathogenesis of salpingitis [38].…”

Section: Hsp60 Specific T-cell Response In Animalsmentioning

confidence: 92%

Heat Shock Protein 60 Specific T‐Cell Response in Chlamydial Infections

Kinnunen

Surcel²

2001

Scand J Immunol

View full text Add to dashboard Cite

Heat shock proteins (HSPs) of most pathogens, including Chlamydia, are major immune targets of both humoral-and cell-mediated immune mechanisms. During the last decade, many investigators have focused their research to elucidate the complex relationship of chlamydial HSPs, especially chlamydial HSP60, and the host immune response. A central issue is whether the pathologic mechanisms in chronic chlamydial diseases are associated with an enhanced immune response to chlamydial HSP60 which can mediate tissue destruction through cytotoxic reactions, or whether they are related to the Th2 type of response that eventually leads to partial or temporary suppression of an effective antichlamydial response. Our review highlights the available knowledge between immune responses to chlamydial HSP60 and chronic chlamydial infections in human.

show abstract

“…1 Adanya heat shock protein 60 (hsp) Chlamydia, reaksi imun tipe lambat serta pelepasan sitokin merupakan mekanisme yang menimbulkan kerusakan pada tuba fallopi. 2,3 Prevalensi infeksi C. trachomatis pada wanita Eropa berkisar 1,7-17%. 4 Pada wanita infertil, prevalensi infeksi ini lebih tinggi.…”

Section: Pendahuluanunclassified

Tingginya Infeksi Chlamydia trachomatis pada Kerusakan Tuba Fallopi Wanita Infertil

Sariroh

Primariawan

2015

MOG

View full text Add to dashboard Cite

Tujuan: Untuk mengetahui gambaran infeksi C. trachomatis pada kerusakan tuba fallopi wanita infertil. Bahan dan Metode: Penelitian ini melibatkan 42 wanita yang menjalani laparoskopi dalam tata laksana infertilitasnya. Infeksi C. trachomatis diperiksa menggunakan metode PCR dari usapan endoserviks dan IgG C. trachomatis diperiksa menggunakan metode ELISA dari darah vena. Kondisi tuba fallopi dilakukan evaluasi saat laparoskopi. Hasil: Didapatkan C. trachomatis sebesar 14,29% dari PCR usapan endoserviks dan 38,10% dari IgG C. trachomatisdi darah. Sebagian besar tuba fallopi pada subyek dengan infeksi C. trachomatis menunjukkan kerusakan, ditandai adanya adhesi perituba, oklusi tuba, fimosis fimbria atau hidrosalping saat laparoskopi. IgG C. trachomatis berbeda signifikan pada kerusakan tuba fallopi (p = 0. 01), sedangkan endometriosis dan riwayat operasi sebagai faktor risiko tidak didapatkan perbedaan yang signifikan (p = 0. 26 dan p = 0. 27). Subyek dengan IgG C. trachomatis memiliki OR: 5. 5 (95% CI 1. 42-21. 7)untuk terjadi kerusakan tuba fallopi. IgG C. trachomatis memiliki sensitifitas 62,5%, spesifisitas 81,25%, PPV 62,5% dan NPV 81,25% dalam mendeteksi kerusakan tuba fallopi bila dikonfirmasi dengan hasil laparoskopi. Simpulan: Angka kejadian infeksi C. trachomatis pada wanita infertil cukup tinggi. Pemeriksaan IgG C. trachomatis dapat menjadi penanda adanya kerusakan tuba fallopi.Kata Kunci: C. trachomatis, infertilitas, kerusakan tuba fallopi, IgG, PCR Objectives: To know the description of C. trachomatis infection in tubal dysfunction of infertile women Materials and Methods: This study involves 42 women who underwent laparoscopy procedure in their infertility work up. Chlamydia trachomatis was detected using PCR from endocervical swabs and IgG was detected using ELISA from blood. Evaluation of the fallopian tube was done during laparoscopy procedure. Results: C. trachomatis was detected in 14,29% by PCR of endocervical swab and IgGin serum was detected in 38,10%. Most of the infected women in this study have fallopian tube dysfunction, confirmed by laparoscopic visualization of peritubal adhesion, tubal occlusion, fimbriae phimosis, or hydrosalphing. IgG C. trachomatis is significantly different between normal and dysfunctional fallopian tube (p = 0. 01). The occurrence of endometriosis and previous surgery was not statistically different (p = 0. 26 and p = 0. 27). Women with IgG C. trachomatis had OR: 5. 5 to get fallopian tube dysfunction(95% CI, 1. 42-21. 7). IgG C. trachomatis has sensitivity of 62. 5% and specificity of 81. 25%, PPV of 62,5% and NPV of 81,25% in detecting dysfunctional tube compared to laparoscopy. Conclusion:The incidence of C. trachomatis infection is quite high in infertile women. IgG Chlamydia trachomatis can be used as a marker of fallopian tube dysfunction. Keywords: Chlamydia trachomatis, infertility, fallopian tube dysfunction, IgG, PCRCorrespondence: Wafi Sariroh, Departemen Obstetri dan Ginekologi, Fakultas Kedokteran Universitas Airlangga, RSUD Dr. Soetomo, Sur...

show abstract

Demonstration of Delayed Hypersensitivity in Chlamydia trachomatis Salpingitis in Monkeys: A Pathogenic Mechanism of Tubal Damage

Cited by 97 publications

References 10 publications

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Heat Shock Protein 60 Specific T‐Cell Response in Chlamydial Infections

Tingginya Infeksi Chlamydia trachomatis pada Kerusakan Tuba Fallopi Wanita Infertil

Contact Info

Product

Resources

About