Demonstration of Delayed‐type Hypersensitivity in Guinea‐pigs by the Agarose Plate Technique

Abstract

Werdelin, 0. Demonstration of delayed-type hypersensitivity in guinea-pigs by the agarose plate technique. Acta path. microbiol. scand. Sect. C, 83: 52-58, 1975. A recently developed method for demonstration of antigen-dependant migration inhibition, the agarose plate technique, and skin tests were used to demonstrate delayed hypersensitivity towards Mycobacterium tuberculosis and Nocardia farcinica in guinea-pigs. Peritoneal exudate cells from animals immunized with M . tuberculosis and N . farcinica displayed immunologically specific inhibition of migration after stimulation with tuberculin and N. farcinica sensitin, respectively. T h e slight cross reactivity between the two antigens demonstrated by skin tests was also observed with the agarose technique. Previous skin testing did not influence migration inhibition of peritoneal exudate cells. Migration inhibitory activity of supernatants of tuberculin stimulated lymph node cells from guinea-pigs which had been immunized with M . tuberculosis was demonstrated on peritoneal exudate cells from non-immunized animals.

Transfer of Arterial Hypertension by Splenic Cells From Doca‐salt Hypertensive and Renal Hypertensive Rats to Normotensive Recipients

“…about six months after the transfer of splenic cells from DOCA-salt hypertensive rats. The result of the skin test was read after 24 hours (Brcendstrup et at. 1975).…”