Demonstration of Food Proteins in Human Breast Milk by Anaphylactic Experiments on Guinea-Pigs

Shannon, W. Ray

doi:10.1001/archpedi.1921.04120030002001

Cited by 72 publications

(20 citation statements)

References 2 publications

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“…Com parison of the skin reaction sites with those injected with standard dilutions of egg suggested that egg white was present in the milk at a concentration of one part in 6 x i0 6 to 6 x l0 9 (approximately 20 ng/ml-20 pg/ml). Shannon [12] claimed to detect egg protein in breast milk by observing anaphylactic shock when the milk was injected into sensitised guin ea pigs but in similar experiments Stuart [ 13] obtained negative results. At least three reports claim to have demonstrated cow's milk or cereal antigens in human milk by immunoprécipitation in gel [4,6,14).…”

Section: Introductionmentioning

confidence: 99%

The Passage of Maternal Dietary Proteins into Human Breast Milk

Kilshaw

Cant

1984

Int Arch Allergy Immunol

245

147

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Samples of breast milk and serum were taken from 29 women at various stages of lactation before and after they had ingested 1 raw egg and half a pint of cow’s milk. The samples were analysed for cow’s milk and egg proteins using a solid-phase radioimmunoassay. β-Lactoglobulin, ovalbumin (OA) and ovomucoid were detected in breast milk from 10 out of 19, 13 out of 22 and 7 out of 9 women, respectively, in concentrations ranging from 110 pg/ml to 6.4 ng/ml. Maximum levels in breast milk were attained 4 or 6 h after ingestion and in serum 1–2 h earlier. The OA in breast milk was of normal molecular size (43,500 daltons) and indistinguishable from native OA in the radioimmunoassay. Gel filtration of whole breast milk or high molecular weight fractions at pH 2.6 failed to reveal additional OA, suggesting that immune complexes were not present. In serum, OA was detected both in its native form and in immune complexes. The possible significance of these antigens in the suckling infant is discussed.

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Section: Introductionmentioning

confidence: 99%

The Passage of Maternal Dietary Proteins into Human Breast Milk

Kilshaw

Cant

1984

Int Arch Allergy Immunol

245

147

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“…1982;Hill et al 1984). The ability of a dietary protein to be transported antigenically intact in human breast milk has been repeatedly demonstrated (Shannon 1921;Axelsson et al 1986;Makinen-Kilunen et al 1992;Lovegrove et al 1993). Protection against potentially harmful agents is ensured by a number of factors including saliva, gastric acid, peristalsis, mucus, intestinal proteolysis, intestinal microbiota, mucosal immunity and epithelial cell membranes with the intercellular junctional complexes (Sanderson et al 1993).…”

Section: Resultsmentioning

confidence: 99%

Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools

et al. 2009

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Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools and in healthy exclusively breast-fed babies was compared. Total anaerobes, bifidobacteria, lactobacilli, coliform bacteria, enterococci and clostridia were quantified by cultivation methods in feces of 17 full-term exclusively breastfed patients (aged 16.3 +/- 7.4 weeks) with blood-streaked stools and in the control group of 22 healthy fullterm exclusively breast-fed infants (13.7 +/- 6.4 weeks). Specific fluorescence in situ hybridization kits for Bifidobacterium spp. were used for the quantitative detection of bifidobacteria in samples. Control samples had significantly (p < 0.05) higher counts of total anaerobes. Bifidobacteria were not detected in patients' samples in 65 % and in controls in 36 % (p < 0.01). Bifidobacteria counts were also significantly higher in the control group (p < 0.01). Furthermore, clostridia strains were detected only in feces from bifidobacteria-negative infants reaching counts >8 log CFU/g. Lactobacilli were not detected in 65 % patients and in 45 % control samples. However, this difference was not significant as well as the difference in lactobacilli counts. Eosinophilia was observed in 35 % of patients, low IgA concentration in 71 % and also low IgG concentration in 71 %. pANCA positivity was found in 53 % of patients. In conclusion a significant low proportion of bifidobacterial microbiota in patients with blood-streaked stools was shown in comparison with controls.

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“…97 The subepithelial plasma cells of the lamina propria mucosae and lymph nodes do not make IgA initially. The early absorption of protein macromolecules in young animals is well recognized.…”

Section: Recommendations For Managementmentioning

confidence: 99%

Human Milk as a Prophylaxis

Lawrence

2011

Breastfeeding

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Demonstration of Food Proteins in Human Breast Milk by Anaphylactic Experiments on Guinea-Pigs

Cited by 72 publications

References 2 publications

The Passage of Maternal Dietary Proteins into Human Breast Milk

The Passage of Maternal Dietary Proteins into Human Breast Milk

Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools

Human Milk as a Prophylaxis

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