Demonstration of Functional Neuronal β3-Adrenoceptors Within the Enteric Nervous System

Cellek, Selim; Thangiah, R; Bassil, Anna K.; Campbell, C. A.; Gray, Karen M.; Stretton, Jennifer L.; Lalude, Olutunde; Vivekanandan, Shanmugam; Wheeldon, Alan; Winchester, Wendy J.; Sanger, Gareth J.; Schemann, Michael; Lee, Kevin

doi:10.1053/j.gastro.2007.05.009

Cited by 56 publications

(49 citation statements)

References 16 publications

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“…In fact, cisplatin is reported to induce a delay in motility of the gastrointestinal tract in rats (10). It is well-known that CL316243 is a potent β 3 -AR agonist that inhibits cholinergic-induced motility of the gastrointestinal tract and chemically induced diarrhea following oral administration at 1 mg/kg (14). Intravenous administration of CL316243 at 3 mg/kg fails to produce significant changes in heart rate in rats (40).…”

Section: Discussionmentioning

confidence: 99%

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Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

et al. 2010

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Abstract. We investigated the effects of various emetic agents: cisplatin, apomorphine, lithium chloride (LiCl), rolipram, sibutramine, and the β 3 -adrenoceptor (AR) agonist CL316243 on salivary amylase secretion in rats. We also determined the inhibitory effect of granisetron, a 5-HT 3 -receptor antagonist, on cisplatin-induced increased salivary amylase activity and the inhibitory effect of bilateral abdominal vagotomy on increases in salivary amylase activity induced by cisplatin and LiCl. Granisetron was administered 15 min before and 1 h after cisplatin administration. Cisplatin (10 -15 mg/kg, i.v.) increased salivary amylase activity dose-dependently and induced an acute increase from 1.5 h post-treatment with 15 mg/kg. Apomorphine (1 -3 mg/kg, s.c.), LiCl (120 mg/kg, i.p.), rolipram (3 -10 mg/kg, p.o.), and sibutramine (10 mg/kg, p.o.) induced significant increases in salivary amylase secretion. On the other hand, CL316243 did not stimulate salivary amylase secretion. The increased amylase activity induced by cisplatin (15 mg/kg, i.v.) was inhibited significantly by granisetron (1 or 3 mg/kg × 2, i.v.) or tended to be inhibited by bilateral abdominal vagotomy, whereas an increase in amylase activity produced by LiCl was not inhibited by abdominal visceral nerve section. These results suggest that salivary amylase activity is useful as a marker for emesis in rats, a species that does not exhibit vomiting.

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Section: Discussionmentioning

confidence: 99%

“…Sampling points of LiCl, rolipram, and sibutramine were selected based on the onset of increased amylase activity through the time to return to the control level. Sampling points of CL316243 were selected based on the time when the motility of the gastrointestinal tract is inhibited (14).…”

Section: Measurement Of Amylase Activity In Rat Salivamentioning

confidence: 99%

Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

et al. 2010

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“…In a rat model of diarrhea, the ␤ 3 -AR agonist CL316243 decreased castor oilinduced fecal weight (12). On the other hand, ␤ 3 -AR agonist does not alter carbachol-induced contractions in human isolated colon (12).…”

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“…␤ 3 -AR is a member of the family of G-protein-coupled receptors that have been cloned from human, mouse, and rat and are expressed in adipocytes, heart, skeletal muscle, and smooth muscle of the gastrointestinal and urogenital systems. ␤ 3 -AR expression is colocalized with choline acetyl transferase in a majority of the neurons in human colonic myenteric and submucosal plexus (12). The human selective ␤ 3 -AR agonist, solabegron, inhibits cholinergic contractions and enhances release of somatostatin with no effect on carbachol-induced contractions in human isolated colon.…”

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Dose-response effect of a β₃-adrenergic receptor agonist, solabegron, on gastrointestinal transit, bowel function, and somatostatin levels in health

Grudell¹,

Camilleri²,

Jensen³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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Changes in the mRNA expression of osteoblast‐related genes in response to β₃‐adrenergic agonist in UMR106 cells

Nuntapornsak

Wongdee

Thongbunchoo

et al. 2009

Cell Biochemistry & Function

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Activation of adrenergic receptors (AR) was demonstrated to result in either bone gain or bone loss depending on the activated AR subtypes and concentrations of agonists used. While beta(2)-AR agonist was extensively investigated as an osteopenic agent, effects of beta(3)-AR activation on osteoblasts were still elusive. Rat osteoblast-like UMR106 cells were herein found to express several AR subtypes, including beta(3)-AR. After exposure to a low-dose beta(3)-AR agonist BRL37344 (10 nmol L(-1)), UMR106 cells downregulated the mRNA expression of transcription factors Runx2 and Dlx5, which are important for initiation of osteoblast differentiation. Low-dose BRL37344 also decreased the expression ratio of receptor activator of nuclear factor kappaB ligand (RANKL) over osteoprotegerin (OPG), suggesting the protective effect of beta(3)-AR agonist against bone resorption. Alkaline phosphatase expression was markedly decreased, whereas expressions of osteocalcin and osteopontin were increased by 100 nmol L(-1) BRL37344, indicating that beta(3)-AR activation could accelerate the transition of matrix maturation stage to mineralization stage. In conclusion, beta(3)-AR activation in rat osteoblasts induced alteration in the expression of osteoblast-related transcription factor genes as well as genes required for bone formation and resorption. The present results also suggest that, besides beta(2)-AR, beta(3)-AR is another AR subtype responsible for the sympathetic nervous system-induced bone remodeling.

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Demonstration of Functional Neuronal β3-Adrenoceptors Within the Enteric Nervous System

Cited by 56 publications

References 16 publications

Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

Dose-response effect of a β₃-adrenergic receptor agonist, solabegron, on gastrointestinal transit, bowel function, and somatostatin levels in health

Changes in the mRNA expression of osteoblast‐related genes in response to β₃‐adrenergic agonist in UMR106 cells

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Demonstration of Functional Neuronal β3-Adrenoceptors Within the Enteric Nervous System

Cited by 56 publications

References 16 publications

Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

Dose-response effect of a β3-adrenergic receptor agonist, solabegron, on gastrointestinal transit, bowel function, and somatostatin levels in health

Changes in the mRNA expression of osteoblast‐related genes in response to β3‐adrenergic agonist in UMR106 cells

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Product

Resources

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Dose-response effect of a β₃-adrenergic receptor agonist, solabegron, on gastrointestinal transit, bowel function, and somatostatin levels in health

Changes in the mRNA expression of osteoblast‐related genes in response to β₃‐adrenergic agonist in UMR106 cells