Demonstration of highly specific and sensitive antibodies to a naturally occurring tetrahydroisoquinoline alkaloid, salsolidine

Stammel, Waltraud; Müller, R.; Thomas, Helmut

doi:10.1016/0020-711x(93)90248-d

Cited by 4 publications

(1 citation statement)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, diazo methods ( Figure 2 A) are commonly used for reactions containing aromatic amine groups, e.g., cocaine [ 85 ] and atropine [ 59 ]. Stammel et al [ 86 ] reported a method of reductive amination, which is a very effective method for coupling small molecules bearing an amino group to carrier proteins. For haptens containing aldehyde groups, Ishiyama et al [ 87 ] and Putalun et al [ 77 ] used NAIO 4 to oxidize the adjacent dihydroxyl groups on the C-3 sugars of solamargine into aldehyde groups.…”

Section: Synthesis and Identification Of Antigenmentioning

confidence: 99%

Progress in Immunoassays of Toxic Alkaloids in Plant-Derived Medicines: A Review

Ren

Zhang

Wang

et al. 2022

Toxins

View full text Add to dashboard Cite

Plants are the cradle of the traditional medicine system, assuaging human or animal diseases, and promoting health for thousands of years. However, many plant-derived medicines contain toxic alkaloids of varying degrees of toxicity that pose a direct or indirect threat to human and animal health through accidental ingestion, misuse of plant materials, or through the food chain. Thus, rapid, easy, and sensitive methods are needed to effectively screen these toxic alkaloids to guarantee the safety of plant-derived medicines. Antibodies, due to their inherent specificity and high affinity, have been used as a variety of analytical tools and techniques. This review describes the antigen synthesis and antibody preparation of the common toxic alkaloids in plant-derived medicines and discusses the advances of antibody-based immunoassays in the screening and detection of toxic alkaloids in plants or other related matrices. Finally, the limitations and prospects of immunoassays for toxic alkaloids are discussed.

show abstract

Section: Synthesis and Identification Of Antigenmentioning

confidence: 99%

Progress in Immunoassays of Toxic Alkaloids in Plant-Derived Medicines: A Review

Ren

Zhang

Wang

et al. 2022

Toxins

View full text Add to dashboard Cite

show abstract

Enantiomeric separation of tetrahydroisoquinoline alkaloids by high‐performance liquid chromatography with β‐cyclodextrin as chiral selector

1995

Self Cite

View full text Add to dashboard Cite

Tetrahydroisoquinohe alkaloids, which are known to be present not only in plants but also in animals, including mammals, can be considered as condensation products of 2-phenylethylamines (e.g., catecholamines) with aldehydes (e.g., acetaldehyde) or 2-0x0 acids (e.g., pyruvic acid). In this study the possibility of separating the optical isomers of several tetrahydroisoquinolines by high-performance liquid chromatography was investigated. For isosalsolme, tetrahydropapaveroline and laudanosoline a good enantiomeric separation could be achieved by applying P-cyclodextrin-bonded silica as stationary phase in connection with various mobile phases. With respect to laudanosoline, the addition of p-cyclodextrin as chiral selector to the mobile phase using a C,, reversed-phase column as stationary phase revealed an even higher resolution when compared with the chiral columns. All tested tetrahydroisoquinohes which could be well separated into enantiomers bear a hydroxyl group at carbon atom 7 as a common structural feature. Those alkaloids substituted with a methoxy group on position 7 instead of a hydroxyl group (e.g., salsolidine) failed to be resolved into their optical isomers. Therefore, the presence of a hydroxyl group on C7 of the aromatic ring seems to be conducive to steric discrimination. However, the separation results for 1-carboxysalsolinol were unsatisfactory although this molecule possesses a 7-hydroxyl group. In this case the existence of a carboxyl group on C1 reduced the chiral recognition and thus the enantiomeric resolution.

show abstract

Synthetic approaches to carnegine, a simple tetrahydroisoquinoline alkaloid

Bracca

Kaufman

2004

Tetrahedron

View full text Add to dashboard Cite

Demonstration of highly specific and sensitive antibodies to a naturally occurring tetrahydroisoquinoline alkaloid, salsolidine

Cited by 4 publications

References 44 publications

Progress in Immunoassays of Toxic Alkaloids in Plant-Derived Medicines: A Review

Progress in Immunoassays of Toxic Alkaloids in Plant-Derived Medicines: A Review

Enantiomeric separation of tetrahydroisoquinoline alkaloids by high‐performance liquid chromatography with β‐cyclodextrin as chiral selector

Synthetic approaches to carnegine, a simple tetrahydroisoquinoline alkaloid

Contact Info

Product

Resources

About