Demonstration of loss of attenuation and extended field persistence of a live avian metapneumovirus vaccine

Catelli, Elena; Cecchinato, Mattia; Savage, C. E.; Jones, Richard C.; Naylor, C. J.

doi:10.1016/j.vaccine.2006.06.076

Cited by 74 publications

(58 citation statements)

References 31 publications

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“…Em frangos de corte o genoma viral já foi detectado até 28 dias PI em suabes clocais (HESS et al, 2004). Banet-Noach et al (2009) e Cecchinato et al (2010 evidenciaram a presença do aMPV em plantéis de frango de corte vacinado e atribuem essa infecção por um subtipo ainda não incluído em vacinas, ou por uma possível reversão da virulência (CATELLI et al, 2006) ou devido a alta variabilidade genética entre as amostras circulantes a campo (BANET-NOACH et al, 2009;CATELLI et al, 2010). Segundo Torres et al (2014), uma vacina que contenha mais distinção genética poderá permitir uma maior proteção contra o aMPV devido ao maior número de subtipos do vírus presente na vacina.…”

Section: Resultsunclassified

APLICAÇÃO DA TÉCNICA DE RT-PCR PARA Metapneumovírus aviário (aMPV)

Filho¹,

Hentges²,

Grassotti³

et al. 2015

ArqVet

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Os principais hospedeiros do Metapneumovírus aviário (aMPV) são os frangos de corte e perus. O vírus acomete o trato respiratório superior dos perus desencadeando a Rinotraqueíte Viral dos Perus (RVP). O principal objetivo deste trabalho foi padronizar uma técnica de RT-PCR para a detecção do aMPV, por meio do uso do kit AccessQuick™ RT-PCR system (Promega®). Foram utilizados amostras de suabes de traqueia e pulmão de 38 perus comerciais com sintomatologia respiratória e dois suabes oculares de faisão. O RNA viral foi extraído utilizando-se o kit RTP® DNA/RNA Virus Mini Kit (STRATEC Molecular). Em seguida as amostras foram submetidas à RT-PCR One Step, utilizando o kit AccessQuick™ RT-PCR system (Promega®). Todas as 40 amostras testadas por RT-PCR foram negativas, exceto a amostra vacinal que foi utilizada como controle positivo. O aMPV não causa latência em frangos de corte ou perus, logo a excreção viral é limitada. Dessa forma, a ausência da detecção de genoma viral neste estudo pode ser justificada devido à idade que as amostras foram coletadas em perus, com 140 dias no abatedouro, impossibilitando dessa maneira a amplificação do genoma do aMPV. Porém, esse estudo também mostra que a RT-PCR se mostrou eficaz para detectar o genoma viral do aMPV, podendo dessa forma ser utilizado como uma ferramenta de diagnóstico rápido para investigação e estudo de casos de aMPV em rebanho de perus.

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Section: Resultsunclassified

APLICAÇÃO DA TÉCNICA DE RT-PCR PARA Metapneumovírus aviário (aMPV)

Filho¹,

Hentges²,

Grassotti³

et al. 2015

ArqVet

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“…On the other hand, vaccines based on live attenuated viruses are presumed to retain their immunosuppressive properties. Additionally, the virulence of these vaccine viruses may be reversed (Catelli et al 2006), especially in flocks where vaccinated birds live alongside those which have not been subjected to specific immunoprophylaxis against the TRT virus.…”

Section: Immunoprophylaxis Against the Trtmentioning

confidence: 99%

The perspective of immunoprophylaxis and selected immunological issues in the course of the turkey rhinotracheitis

Smiałek¹,

Tykałowski²,

Stenzel³

et al. 2012

Polish Journal of Veterinary Sciences

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This review article presents immunological issues in the course of the turkey rhinotracheitis (TRT) emphasizing local immunity mechanisms, both humoral and cell-mediated, in the upper respiratory system. Studies on the influence of the humoral immunity in the course of infection and vaccinations against TRT have revealed many times the absence of correlation between the titre of specific IgY anti-aMPV (avian Metapneumovirus) antibodies in the serum and in the upper respiratory washings and the immunity against the occurrence of the clinical form of the TRT. Considering the above, T cells are increasingly often regarded as the main factor involved in the upper respiratory immunity against the TRT. However, there have been just a few reports on the role of the T cells in the local immunity processes in the infection with aMPV in turkeys. Additionally, studies of the T-cell-associated immunity against the TRT have given ambiguous results.Immunoprophylaxis issues against the aMPV infections are a significant part of the work where the authors confront current vaccination programmes against the perspectives of use of the future vaccines against the TRT. Future vaccines should face the following criteria: absence of the risk of immunosuppressive effect and reversion of vaccine strains virulence, ease-of-use combined with the possibility of administration of the vaccine to the large numbers of turkeys. The leading role in future vaccination programs for birds against the TRT is likely to be played by the in ovo technique and the recombinant vaccines. Great hopes are also linked with the development of subunit vaccines against the aMPV.

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“…Although vaccination is the main tool for the control and prevention of diseases caused by aMPV, pharmaceutical intervention is required under some circumstances. Furthermore, vaccinated subjects may acquire incomplete protection and/or there may be a reversion of the vaccine virus to pathogenicity (Catelli et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV)

Kohn

Foglio

Rodrigues

et al. 2015

Rev. Bras. Cienc. Avic.

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Avian metapneumovirus (aMPV) is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to the Paramyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 μg mL -1 ) to inhibit the cytopathic effect (CPE) of the virus, expressed as inhibition percentage (IP). The maximum nontoxic concentration (MNTC) of the extracts used in antiviral assay was 25 μg mL -1 for Aspidosperma tomentosum and Gaylussacia brasiliensis, and 2.5 μg mL -1 for Arrabidaea chica and Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica, and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action.

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Demonstration of loss of attenuation and extended field persistence of a live avian metapneumovirus vaccine

Cited by 74 publications

References 31 publications

APLICAÇÃO DA TÉCNICA DE RT-PCR PARA Metapneumovírus aviário (aMPV)

APLICAÇÃO DA TÉCNICA DE RT-PCR PARA Metapneumovírus aviário (aMPV)

The perspective of immunoprophylaxis and selected immunological issues in the course of the turkey rhinotracheitis

In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV)

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