Demonstration of Peritrichous Flagella of Tyzzer's Disease Organism

Fujiwara, Kôsaku; Kurashina, H; Matsunuma, Naochika; Takahashi, Reiji

doi:10.1111/j.1348-0421.1968.tb00407.x

Cited by 13 publications

(1 citation statement)

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“…Motility was first described by Craigie (I) with the organisms from embryonated eggs, and peritrichous flagella were first demonstrated with those from experimentally infected mice (6). Observations were later obtained in natural cases of rats (12), foals (15), and cats (II).…”

Section: Discussionmentioning

confidence: 99%

Plaque Formation by Tyzzer's Organism in Primary Monolayer Culture of Mouse Hepatocytes

Kawamura

Taguchi

Fujiwara

1983

Microbiology and Immunology

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Tyzzer's disease organism propagated on primary monolayer cultures of mouse hepatocytes and produced definite plaques. In phase contrast microscopy, the organisms were motile in the plaques. Plaque formation was inhibited by antiserum. After serial plaque cloning the organisms still had virulence in mice. To establish a standard plaquing procedure, factors affecting plaque formation were studied. The critical factors in plaque formation were the culture period of host cells before inoculation, medium for suspending the organisms, and temperature and time of infection. A 24 to 36 hr-preculture of host cells and trypticase soy broth (BBL) as the suspending medium gave the best results. The optimal conditions for infection were 37 C for 90 min. The plaquing efficiency was higher when a larger volume of inoculum was applied to host cell monolayers, suggesting that the organism played an active role in the initial stage of infection.

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Section: Discussionmentioning

confidence: 99%