Demonstration of R Factors From
            <i>Pseudomonas aeruginosa</i>

Iyobe, Shizuko; Hasuda, Katsumi; Fuse, Akihisa; Mitsuhashi, Susumu

doi:10.1128/aac.5.6.547

Cited by 34 publications

(19 citation statements)

References 6 publications

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“…As reported previously (Iyobe et al 1974), there are two groups of MAC distribution in P. aeruginosa strains, i.e., low and high resistance group. We selected highly resistant strains of P. aeruginosa..…”

Section: Methodsmentioning

confidence: 63%

“…It is known that R plasmids are conjugally transferable among most genera of the Enterobacteriaceae (Mitsuhashi 1971), but R plasmids in P. aeruginosa are mostly transferable among the isogenic strains of P. aeruginosa (Iyobe et al 1974). From these results, we can conclude that newborn infants were contaminated in the hospital with bacteriaincluding drug-resistant strains, whic carried at a high frequency R plasmids with resistance patterns of (TC.CM.SM.SA) or (TC.CM.SM.SA.…”

Section: Results and Commentsmentioning

confidence: 99%

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Isolation of drug-resistant bacteria from newborn infants.

Mitsuhashi¹,

Kaneko²

1977

Tohoku J. Exp. Med.

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Section: Methodsmentioning

confidence: 63%

Section: Results and Commentsmentioning

confidence: 99%

Isolation of drug-resistant bacteria from newborn infants.

Mitsuhashi¹,

Kaneko²

1977

Tohoku J. Exp. Med.

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“…The molecular weight of Rmsl39 PCase was found to be similar to those of PCases belonging to types I and II and different from that (46,000) (5,12,13), except in a few cases such as RP4 (20,24). It is interesting to note that there are two groups of PCases mediated by R factors in P. aeruginosa strains, types I and IV.…”

Section: Methodsmentioning

confidence: 87%

Plasmid-Mediated Penicillin Beta-Lactamases in Pseudomonas aeruginosa

Sawada

Yaginuma

Tai

et al. 1976

Antimicrob Agents Chemother

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A penicillin 13-lactamase (PCase) was extracted from Pseudomonas aeruginosa Rms139+ and purified by means of column chromatography. The isoelectric point of Rmsl39 PCase was 5.7 and its molecular weight was 22,500 ± 1,000. The optimal pH for the hydrolysis of benzylpenicillin was 7.0 to 7.5 and the optimal temperature was 45 C, with the PCase also showing high activity against carbenicillin. It is concluded that this enzyme is a new type of penicillin ,B-lactamase different from the type I, II, or III R plasmid-mediated PCases reported previously.Penicillin resistance conferred by R factors in gram-negative bacteria has been reported by many investigators, and the production of penicillinase (PCase) is known to be one of the main factors of penicillin resistance in clinical isolates carrying R factors (2,3,8,10,18,21).We reported previously three types of PCase whose synthesis was mediated by R factors, the PCases being different in their physicochemicalr enzymological, and immunological properties (10,21,26,27). A type I PCase mediated by R-GN14 (renamed Rms212) is the most common PCase demonstrated in gram-negative enteric bacteria carrying R factors, and is quite similar to the PCase produced by ampicillin-resistant strains of Klebsiella pneumoniae (10,21,27) and to the PCase mediated by the R-TEM plasmid (9). A type II PCase mediated by R-GN238 (renamed Rms213) and the type Ill PCase mediated by Rte16 (26) and R1818 (6) are different from type I PCase in their enzymological and physicochemical properties, i.e., behavior to various inhibitors, isoelectric points, and kinetic parameters (Km and Vma,).Sykes and Richmond (24, 25) and Fullbrook et al. (11) reported PCases mediated by R1822 and R3245 demonstrated in Pseudomonas aeruginosa strains that were very similar to the type I PCase in the substrate profiles, antiserum neutralization curves, and electrophoretic mobilities.This paper deals with the enzymological and physicochemical properties of PCases mediated by R factors from P. aeruginosa and conmpares them with the other known R-factor PCases for possible phylogenetic relationships. MATERIALS AND METHODSBacterial strains. P. aeruginosa strains were isolated from clinical sources, and 28 strains resistant to more than 400 ,ug of carbenicillin (CPC) per ml were selected from 150 strains examined. P. aeruginosa ML4259 (leu-arg-ilv-his-trp-kan-r) (kan, kanamycin; r, resistant) and P. aeruginosa ML4262 (his-ilv-met-trp-rif-r) (rif, rifampin) were used as the recipients for conjugal transfer ofthe R factors.Culture and harvesting of organisms. P. aeruginosa was grown overnight in 400 ml of nutrient broth at 37 C. The culture was diluted with 4 liters of fresh broth and then grown at the same temperature under aeration until the absorbance at 620 nm (Hitachi Perkin-Elmer spectrophotometer) reached 0.7 to 0.9. The cells were then harvested by centrifugation and washed once with 0

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“…The introduction in clinical medicine of carbenicillin (Cb) and gentamicin (Gm), antibiotics with special antipseudomonal activity, and the emergence in Pseudomonas of resistance towards these drugs have stimulated an interest in detecting the episomal nature of these resistances (3,4,27). Such studies have revealed that R-plasmids carrying genes coding for enzymes inactivating Cb or the aminoglycosides have evolved in Pseudomonas in several parts of the world (4,8,12,18,19,22,23,25,(35)(36)(37). Most of the Pseudomonas R-plasmids either have been found in Pseudomonas isolated from wounds, after long use of these antipseudomonal drugs in topical applications, or were transferable intragenerically.…”

mentioning

confidence: 99%

Multiresistant Plasmids from Pseudomonas aeruginosa Highly Resistant to Either or Both Gentamicin and Carbenicillin

Kontomichalou

Papachristou

Angelatou

1976

Antimicrob Agents Chemother

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High-level resistance to gentamicin and carbenicillin was found in 30 and 10.7%, respectively, of Pseudomonas aeruginosa strains, especially in isolates from urine. In 23 out of 25 strains tested, these resistances were R mediated and linked to multiresistant plasmids, carrying genes for resistances to five other aminoglycosides, tobramycin, kanamycin, neomycin, streptomycin, and spectinomycin, and for resistances to chloramphenicol, tetracycline, sulfonamides, and mercury chloride. Carbenicillin resistance was unstable in Pseudomonas, and in its presence the multiresistant plasmids had a host range extended to the Enterobacteriaceae (group I plasmids). Otherwise they were transferable intragenerically only (group II plasmids). The extended host range plasmids were, as a rule, in fi-incompatibility class A-C. Segregants incompatible with both class A-C and P plasmids were detected. The 8-lactamase specified by the carbenicillin marker was of the TEM-like type. Multiple linkages of resistance determinants to the aminoglycosides were concomitantly present in most of the plasmids. Results from the bioassay indicated the presence of at least two aminoglycoside-inactivating enzymes.

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Demonstration of R Factors From Pseudomonas aeruginosa

Cited by 34 publications

References 6 publications

Isolation of drug-resistant bacteria from newborn infants.

Isolation of drug-resistant bacteria from newborn infants.

Plasmid-Mediated Penicillin Beta-Lactamases in Pseudomonas aeruginosa

Multiresistant Plasmids from Pseudomonas aeruginosa Highly Resistant to Either or Both Gentamicin and Carbenicillin

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