Demonstration of the high‐affinity IgE receptor (Fc_ɛRI) on Langerhans' cells of diseased nasal mucosa

Abstract

Langerhans' cells in the skin have recently been shown to bind IgE molecules via the high-affinity IgE receptor (Fc epsilon RI). Using two highly specific antibodies against the antibody-binding alpha-chain of this receptor, 29C6 and 6F7, we demonstrate by immunohistochemistry and immunoelectron microscopy that Langerhans' cells of diseased nasal mucosa can express the Fc epsilon RI. Tissue sections from hyperplastic nasal conchae and nasal polyps of atopic and nonatopic patients have shown no basic differences in epithelial Fc epsilon RI-bearing cells. Only a few cells expressed the low-affinity IgE receptor (Fc epsilon RII) (Tü1 antibody) in some sections. These findings suggest that Langerhans' cells play an important role in the induction of transepithelial IgE-mediated allergy and in the mediation of inflammation of the nasal mucosa via their Fc epsilon RI.

Mast cells and sphingosine‐1‐phosphate underlie prelesional remodeling in a mouse model of eczema

“…Importantly, the α chain is responsible for IgE binding to its receptor and initiates MC activation during allergic responses . Although expressed by Langerhans and other cells, we reasoned that skin‐resident MC could also contribute to the enhanced FcεRIα mRNA detection in these samples. Microscopy examination of methylene blue (MB)‐stained skin sections revealed that metachromatic MCs were distributed throughout the skin layers, irrelevant of treatment (Figure B,C).…”

Important research questions in allergy and related diseases: 3‐chronic rhinosinusitis and nasal polyposis – a GA2LEN study

“…A recent manuscript (47) on a very few patients showed the expression DC‐LAMP and DC‐SIGN in nasal biopsies. A second study (48) showed the presence of IgE+ Langerhans’ cells (CD1a). Other than that these cells types are present, no conclusion on their role in CRS/NP were drawn.…”

Local IgE Production in the Nasal Mucosa: A Review

“…Langerhans cells are able to bind allergens to IgE via the high-affinity receptor for IgE. [23][24][25][26][27] This has been shown to be specific. 25 Immunodouble staining methods using CDI and anti-IgE-FITC on sections of the nasal mucosa of patients with allergic rhinitis revealed IgE on 20-40% of the CD 1+ cells in the epithelium and occasionally on CDI + cells in the lamina pro-300 pria.2 8 Although the absolute number of IgE+/CDI + cells increases during the grass pollen season, no difference was observed in the percentage of IgE+/CDl + cells during, before or after the grass pollen season.…”