Demonstration of the prostatic origin of metastases.An immunohistochemical method for formalin-fixed embedded tissue

Jöbsis, A. C.; Vries, G. P. de; Anholt, Robert R. H.; Sanders, Gerard T.

doi:10.1002/1097-0142(197805)41:5<1788::aid-cncr2820410521>3.0.co;2-d

Cited by 136 publications

(18 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, we found both PSA and PSAP to be 100% specific for prostatic adenocarcinoma. Like others, we found a rare case of high-grade prostatic adenocarcinoma that was negative for both PSA and PSAP (10,11,(13)(14)(15). Similar to previous reports, none of our cases of urothelial carcinoma of the urinary bladder expressed PSA or PSAP (11,12).…”

Section: Discussionsupporting

confidence: 77%

Immunophenotype of High-Grade Prostatic Adenocarcinoma and Urothelial Carcinoma

et al. 2000

View full text Add to dashboard Cite

Morphologic features alone can usually be used to distinguish prostatic adenocarcinoma and urothelial carcinoma of the urinary bladder. Poorly differentiated tumors, however, can occasionally have features of both neoplasms, making determination of site of origin difficult. No study has provided a panel of antibodies to assist in the distinction of these two tumors. For this study, 73 examples of moderately and poorly differentiated prostatic adenocarcinoma and 46 examples of high-grade urothelial carcinoma were obtained from radical resection specimens. Immunohistochemical studies were performed using the following panel of antibodies: cytokeratin (CK) 7, CK 20, 34␤E12, Leu M1, carcinoembryonic antigen (CEA)m, CEAp, p53, Leu 7, prostate-specific acid phosphatase (PSAP), prostate-specific antigen (PSA), and B72.3. Mucicarmine was also performed. Intermediate and high-grade prostatic carcinoma were compared and then high-grade prostatic carcinoma was compared with high-grade urothelial carcinoma. PSA and PSAP each stained 94% of prostatic adenocarcinomas, but no urothelial carcinomas. Leu 7 stained 94% of prostate and 17% of urothelial carcinomas. Over half of the urothelial carcinomas showed positivity for 34␤E12 (65%), as did two cases of prostatic carcinoma (6%). Eighty-three percent of urothelial carcinomas and 12% of prostatic adenocarcinomas stained with CK 7. Forty-one percent of urothelial carcinomas and 12% of prostatic carcinomas were reactive for CEAm, and p53 stained 33% and 3% of urothelial and prostatic adenocarcinomas, respectively. No significant difference was seen in the expression of CEAp, CK 20, B72.3, Leu M1, or mucicarmine between prostate and urothelial carcinoma. We propose a panel of six antibodies to assist in the distinction of high-grade prostatic adenocarcinoma from high grade urothelial carcinoma: PSA, PSAP, 34␤E12, Leu 7, CK 7, and p53. The first three antibodies should be used initially; if results are negative, the remaining antibodies may be employed.KEY WORDS: 34␤E12; CK 7; immunohistochemistry; Leu 7; prostatic adenocarcinoma; PSA; PSAP; urothelial carcinoma. Mod Pathol 2000;13(11):1186 -1191Prostatic adenocarcinoma and urothelial carcinoma of the urinary bladder are the most common and fifth most common cancers in men, respectively (1). Both neoplasms usually are found in patients older than 50 years; in a subset of patients, both neoplasms occur synchronously or metachronously. Indeed, for patients who undergo cystoprostatectomy for urothelial carcinoma of the bladder, the reported incidence of concurrent prostatic adenocarcinoma is reported to be from 46 to 70% (2-4). At Memorial Sloan-Kettering Cancer Center (MSKCC), we frequently encounter patients with bladder neck tumors that clinically and pathologically are not clearly urothelial carcinoma or prostatic adenocarcinoma. Cystoscopy typically reveals a mass at the bladder neck, whereas the remainder of the bladder is unremarkable. Biopsies show a tumor that is often high grade and has morphologic features of both urothelia...

show abstract

Section: Discussionsupporting

confidence: 77%

Immunophenotype of High-Grade Prostatic Adenocarcinoma and Urothelial Carcinoma

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Recently, antibodies have been derived that react with antigenic determinants found either characteristically or specifically in certain carcinoma cell types. For example (see Table 1), antibodies directed against prostatic acid phosphatase 6 and prostatic epithelial surface antigens 7 react with both benign prostatic epithelium and neoplastic prostatic epithelium in most instances. In the case under discussion, these antibodies were applied to tissue sections and showed strong reactivity with the neoplastic cells (Figs.…”

Section: Discussionmentioning

confidence: 99%

Capsule Dermatopathology: Origin of Malignant Epithelial Neoplasms of the Skin

Murphy

1984

The Journal of Dermatologic Surgery and Oncology

View full text Add to dashboard Cite

“…Using this technique various cytoplasmic and membrane antigens have been demonstrated (see Table 1). These include immunoglobulin heavy and light chains, intermediate filaments (Ramaekers et al, 1983), carcino-embryonic antigen, prostatic acid phosphatase (Jobsis et al, 1978), epithelial membrane antigen (Heyderman et al, 1979), human milk fat globule (Arklie et al, 1981), thyroglobulin (Burt & Goudie, 1979), Leu-M1 (Hsu & Jaffe, 1984), leucocyte common antigen (Pizzolo et al, 1980), haemoglobin, blood group antigens, factor VIII-related antigen , elastase (Kramps et al, 1984), muramidase, a 1 anti-chymotrypsin and S-100 (Weiss et al, 1983). For general reviews of these techniques see and Taylor & Kledzik (1981).…”

Section: Immunohistochemical Staining Methods For Resin Sectionsmentioning

confidence: 99%

Immuno‐ and enzyme‐histo/cytochemical analysis of resin sections and cell suspensions: a comprehensive diagnosis of bone marrow on a single aspiration sample

Sandt¹,

Herman²,

Lindeman³

1986

Journal of Microscopy

View full text Add to dashboard Cite

Immuno-and enzyme-histokytochemical analysis of resin sections and cell suspensions: a comprehensive diagnosis of bone marrow on a single aspiration sample by M. M. VAN DE SANDT, C. J. HER MAN^^^ J. L~~~~~~~~S t i c h t i n g S a~w e r k i n g Delftse Ziekenhuizen, Reynier de Graejbeg 7,2625 AD Delft, The Netherlands KEY WORDS.Bone marrow, resin embedding, immuno-histochemistry, enzymehistochemistry, haematology . SUMMARY A protocol is described in which a single bone marrow aspiration specimen is used to prepare resin sections and cell suspensions. Using this protocol, a full battery of morphological, enzyme-histochemical, immuno-histochemical and cyto-pathological techniques can be applied. This allows a definitive bone marrow diagnosis to be established without resorting to bone marrow biopsy in the majority of patients.

show abstract

Demonstration of the prostatic origin of metastases.An immunohistochemical method for formalin-fixed embedded tissue

Abstract: An indirect immunohistochemical technique is

Cited by 136 publications

References 13 publications

Immunophenotype of High-Grade Prostatic Adenocarcinoma and Urothelial Carcinoma

Immunophenotype of High-Grade Prostatic Adenocarcinoma and Urothelial Carcinoma

Capsule Dermatopathology: Origin of Malignant Epithelial Neoplasms of the Skin

Immuno‐ and enzyme‐histo/cytochemical analysis of resin sections and cell suspensions: a comprehensive diagnosis of bone marrow on a single aspiration sample

Contact Info

Product

Resources

About