Demonstration of the Rat Lymphatic Vessels Using Immuohistochemistry and In Situ Hybridization for 5^|^apos;-Nucleotidase

Shimoda, Hiroshi; Takahashi, Yoshiaki; Kajiwara, Tooru; Kato, Seiji

doi:10.2220/biomedres.24.51

Cited by 7 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This coincides with the results that the regrowing lymphatics in the lesion displayed an accumulation of 5'-Nase transcripts. These findings suggest that the 5'-Nase may be correlated to the functional maturation of the regenerating lymphatics, because 5'-Nase is considered to facilitate membrane transport of lymph (Shimoda et al 2003). Fig.…”

Section: '-Nase In the Regenerating Lymphaticsmentioning

confidence: 87%

“…5'-Nase mRNA was preferentially expressed in the lymphatic vessels in the intestinal muscle coat, as in central lacteals (Shimoda et al 2003). Few or no signals were seen in any other tissue elements including blood vessels.…”

Section: In Situ Hybridization For 5'-nase Mrnamentioning

confidence: 93%

“…In situ hybridization Some tissue sections were processed for in situ hybridization histochemistry to examine the cellular distribution of 5'-Nase and VEGF-C mRNA signals, according to the method described in our previous report (Shimoda et al 2003). Two kinds of antisense oligonucleotide probes were prepared for each procedure of in situ hybridization, which were complementary to nucleotide residues 693-737 (TGGCACACTTCAT-GAACCTCCTGGGCTACGATGCCATGGCACTGG) and 1078-1122 (TGCCCAGAAGGTGAGAGGCGTGGACGTCGTGGTGG-GAGGACACAC) of the rat 5'-Nase cDNA (GenBank accession no.…”

Section: Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Regrowth of lymphatic vessels following transection of the muscle coat in the rat small intestine

Shimoda

Takahashi

Kato

2004

Cell and Tissue Research

Self Cite

View full text Add to dashboard Cite

Regeneration of lymphatic vessels after transection of the muscle coat in the rat jejunum was studied by histochemical methods. The lymphatic regrowth occurred behind the regeneration of blood vessels. Enzyme histochemistry for 5'-nucleotidase (5'-Nase) demonstrated the manner of lymphatic regrowth, which was essentially attributed to vascular sprouting from preexisting lymphatics, and structural changes of the endothelial cells indicating their high migratory potential. The lymphatic regeneration progressively advanced with vascular maturation throughout the experimental period. The expression of 5'-Nase in the regenerating lymphatics was increased in proportion to their growth. VEGF-C, a highly specific lymphangiogenic factor, was highly expressed in a subpopulation of interstitial cells, being close to the regrowing lymphatics with immunoreactivity of its receptor, VEGFR-3, in the regenerative area. The present findings suggest that transection of the intestinal muscle coat affords a useful experimental model for the investigation of lymphatic regeneration in tissue repair and that the interstitium may play a crucial role in lymphangiogenesis.

show abstract

Section: '-Nase In the Regenerating Lymphaticsmentioning

confidence: 87%

Section: In Situ Hybridization For 5'-nase Mrnamentioning

confidence: 93%

Section: Immunohistochemistrymentioning

confidence: 99%

See 1 more Smart Citation

Regrowth of lymphatic vessels following transection of the muscle coat in the rat small intestine

Shimoda

Takahashi

Kato

2004

Cell and Tissue Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although many of the mice lacking Ang2 die within 2 weeks of birth (Gale et al 2002), we have obtained multiple surviving adult mutant animals with severe lymphatic dysfunction (Hunter et al 2006) in order to examine the fine structure of their lymphatic capillaries. Hence, we have explored the precise structure of the lymphatic capillaries in Ang2 knockout and Ang1 knockin mice by focusing on the lymphatics in the digestive organs, which contain a welldeveloped lymphatic vasculature (Shimoda 1998;Shimoda and Kato 2006;Shimoda et al 1997Shimoda et al , 2001Shimoda et al , 2003Shimoda et al , 2004.…”

Section: Introductionmentioning

confidence: 99%

Abnormal recruitment of periendothelial cells to lymphatic capillaries in digestive organs of angiopoietin-2-deficient mice

et al. 2007

Self Cite

View full text Add to dashboard Cite

The fine structure of lymphatic capillaries in the digestive organs of angiopoietin-2 (Ang2) knockout mice was studied by using both immunohistochemistry and electron microscopy. The genetic deletion of Ang2 yielded hypoplasia and disorganization of the lymphatic capillaries, with their shapes being irregular, and an aberrant recruitment of vascular periendothelial cells immunopositive for smooth muscle actin to the lymphatic capillaries. The abnormal lymphatic periendothelial cells were considered to be a type of pericyte for the lymphatic capillaries after the deletion of Ang2, because they were ultrastructurally characterized by abundant thin myofilaments in their cytoplasm and long cytoplasmic extensions similar to those shown by blood vascular pericytes. The genetic replacement of Ang2 with Ang1 rescued the defects, viz., the disorganization and disordered structure of the lymphatic capillaries. The present findings suggest that Ang2 serves the morphogenesis of lymphatic capillaries as an agonist for the receptor, Tie2, and that Ang1 can replace Ang2 in guiding lymphatic formation and development.

show abstract

“…But the cumulative recovery in tumor-bearing mice was slightly higher than that in healthy mice, indicating enhanced lymphatic uptake in tumor-bearing mice because the tumorinduced lymphangiogenesis (Shimoda et al, 2003) promotes the lymphatic uptake (Sleeman et al, 2009).…”

Section: Healthy Micementioning

confidence: 93%

Study on enhanced lymphatic exposure of polyamidoamin-alkali blue dendrimer for paclitaxel delivery and influence of the osmotic pressure on the lymphatic targeting

Yang

Mao

et al. 2015

Drug Delivery

View full text Add to dashboard Cite

In this study, paclitaxel (PTX)-loaded polyamidoamin-alkali blue (PTX-P-AB) was prepared in order to investigate the intralymphatic targeting ability and anti-cancer effect after subcutaneous (s.c.) administration. The physicochemical properties and in vitro drug release were evaluated. The lymphatic drainage and lymph nodes (LNs) uptake were examined by pharmacokinetics and distribution recovery of PTX in plasma, LNs, injection site (IS) and tissues after s.c. injection in healthy mice and in tumor-bearing mice. The osmotic pressure of PTX-P-AB affecting the lymphatic targeting was studied. The anti-tumor activity of PTX-P-AB was investigated in mice bearing S180 metastatic tumors. Results showed that PTX-P-AB with suitable and stable physicochemical properties could be used for in vivo lymphatic studies, and displayed the more rapid lymphatic absorption, the higher AUC value in LNs, the longer LNs residence time and the higher metastasis-inhibiting rate compared with Taxol Õ . Enhanced lymphatic drainage from the IS and uptake into lymph by increasing the osmotic pressure of PTX-P-AB indicated that PTX-P-AB possesses the double function of lymphatic tracing and lymphatic targeting, and suggested the potential for the development of lymphatic targeting vectors and the lymphatic tracer for treatment and diagnosis. KeywordsLymphatic drainage and uptake, lymphatic targeting, polyamidoamin-alkali blue, osmotic pressure, subcutaneous administration History

show abstract

Demonstration of the Rat Lymphatic Vessels Using Immuohistochemistry and In Situ Hybridization for 5^|^apos;-Nucleotidase

Cited by 7 publications

References 0 publications

Regrowth of lymphatic vessels following transection of the muscle coat in the rat small intestine

Regrowth of lymphatic vessels following transection of the muscle coat in the rat small intestine

Abnormal recruitment of periendothelial cells to lymphatic capillaries in digestive organs of angiopoietin-2-deficient mice

Study on enhanced lymphatic exposure of polyamidoamin-alkali blue dendrimer for paclitaxel delivery and influence of the osmotic pressure on the lymphatic targeting

Contact Info

Product

Resources

About