Demonstration von fetalem H�moglobin in den Erythrocyten eines Blutausstrichs

Kleihauer, E.; Braun, H.; Betke, Klaus

doi:10.1007/bf01481043

Cited by 774 publications

(125 citation statements)

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“…FMH was determined according to the method of Kleihauer-Betke's [9]. Two ml of maternal blood was obtained into EDTA bottle within 2 hours of the postpartum period.…”

Section: Methodsmentioning

confidence: 99%

Role of Placenta Parameters in Predicting Significant Feto-Maternal Haemorrhage

Adeniji¹,

Atanda²,

Muhibi³

et al. 2013

IJCM

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Purpose: Feto-maternal haemorrhage (FMH) is a complication of pregnancy and large FMH may lead to life-threatening anaemia in the fetus or newborn. In addition, exposure of Rhesus (Rh) D negative women to small amounts of fetal Rh D positive red cells during pregnancy or delivery may result in sensitization with its attendant problems of isoimmunisation. In most cases, the cause of FMH IS unknown. Through this study, we sought to determine if placental weight & diameter have any direct relationship with incidence and severity of FMH. Methods: This was a prospective study of parturients for presence of fetal red cells in the maternal blood circulation. The prepared slide was processed as in the acid elution test described by Kleihauer-Betke. The FMH was calculated using Mollison formula. Baseline data included maternal biodata, blood group, Rh D factor, placenta weight and diameter. Data generated were analysed with Frequency tables, cross-tabulations and Odd ratio and confidence intervals as appropriate. Results: Three hundred parturients were studied. However, only two hundred and ninety-five parturients were analysed, with five excluded due to lysed blood samples. A total of 52 parturients (17.63%) had demonstrable FMH, of which 8 (2.71%) were large FMH (>15 ml foetal cells). Both the placenta weight (P < 0.005) and diameter (P < 0.042) were significantly associated with incidence of FMH, more with placenta weight than diameter. Incidence of demonstrable FMH was 24.12% (48/199) in the group with placenta weight greater than 500 g, in contrast to 4.17% (4/96) in the group with weight of placenta below or equal to 500 g. All the 8 parturients with large FMH had placenta weights greater than 500 g. Placenta diameters were greater than 22 cm in 41/197 (20.81%) who had demonstrable FMH, compared with 11/98 (11.23%) whose diameter was less than 22 cm. Conclusion: Both the placenta weight and diameter are significant predictors of FMH in parturients. However, placenta diameter appears to be a minor predictor. These are factors that can be assessed antenatally by ultrasonography and in conjunction with other known obstetric factors, may possibly be considered in risk-based scoring system for predicting feto-maternal haemorrhage.

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“…FMH was determined according to the method of Kleihauer-Betke's [9]. Two ml of maternal blood was obtained into EDTA bottle within 2 hours of the postpartum period.…”

Section: Methodsmentioning

confidence: 99%

Role of Placenta Parameters in Predicting Significant Feto-Maternal Haemorrhage

Adeniji¹,

Atanda²,

Muhibi³

et al. 2013

IJCM

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“…The technique used to demonstrate fetal cells in the maternal circulation was a modification of the acid-elution method of Kleihauer, Braun, and Betke (1957). The fetal erythrocytes containing haemoglobin F stain a darker red colour than the maternal erythrocytes.…”

Section: Methodsmentioning

confidence: 99%

Transplacental Passage of Fetal Red Cells in Abortion; Increased Incidence After Curettage and Effect of Oxytocic Drugs

Katz¹

1970

Obstetrical & Gynecological Survey

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Suppression of Lactation-HTLim 1t al.,s, BRrInSH 84 1 1 coer16 uppression of actation-H~akim et al. MEDICAL JOURNAL a thrombus (Wessler and Reimer, 1960). Daniel et al. (1968) suggested that very high levels of plasma factor IX may be related to the development of puerperal venous thrombosis. It would appear reasonable to avoid the use of therapeutic agents which give rise to excessive or prolonged increases in factor IX levels during the puerperiuim. If lactation is to be suppressed with oestrogen it is preferable to use the smallest dose that proves effective.The present study has shown that intramuscular hexoestrol has a theoretical advantage over oral stilboestrol in that the increase in factor IX levels is not so great and is of a shorter duration. With most hexoestrol-treated patients plasma factor IX has reverted to normal by the time they leave hospital.We are grateful to Dr. W. R. Pitney for instruction in the technique of factor IX estimations and for his advice at all stages, and to Ortho Pharmaceutical Limited for a supply of factor-IXdeficient plasma and for financial assistance with the study. British Medical Journal, 1969, 4, 84-86 Summary: In a study of early abortions (less than 16-week pregnancies) no significant increase in fetomaternal haemorrhage was found in patients having either threatened or incomplete abortions. A statistically significant increase in fetal cells in the maternal circulation, however, occurred after curettage. The administration of oxytecic drugs in conjunction with curettage in cases of incomplete abortion did not increase the incidence of transplacental passage of fetal erythrocytes when compared with curettage alone. Of the 81 patients curetted following abortion four had a feto-maternal haemorrhage of more than 0-2 ml. The largest amount of fetal blood found in the maternal circulation was 0 4 to 0 5 ml. Preliminary data evaluating the indirect Coombs test and enzyme-treated red cells in Rh-negative post-abortion cases suggest that this amount of blood is not a primary immunizing dose but a " booster " to preformed antibody.

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“…In acid conditions, fetal and adult hemoglobin have differences in solubility properties. Hemoglobin F (HbF) resists to acid elution and fetal RBCs are stained in bright pink, while hemoglobin is eluted from adult RBCs that appear as ghost cells (3). Nevertheless, the KBT is labor-intensive and inaccurate, theoretically leading to inappropriate dose of anti-RhD immunoglobulin (4).…”

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confidence: 99%

Evaluation of FMH QuikQuant for the detection and quantification of fetomaternal hemorrhage

Pastoret

Priol

Fest

et al. 2012

Cytometry Part B Clinical

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Background: The Kleihauer-Betke test (KBT) is the most widely used assay for fetomaternal hemorrhage (FMH) detection in rhesus D negative women. In the current study, we sought to evaluate the performance of a flow cytometry (FCM) kit (FMH QuikQuant) using an anti-HbF antibody.Methods: Eighty-three pregnant women, 58 umbilical cord blood (UCB) dilutions in adult blood, and 6 control samples were tested in parallel with FCM and KBT.Results

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Demonstration von fetalem H�moglobin in den Erythrocyten eines Blutausstrichs

Cited by 774 publications

References 0 publications

Role of Placenta Parameters in Predicting Significant Feto-Maternal Haemorrhage

Role of Placenta Parameters in Predicting Significant Feto-Maternal Haemorrhage

Transplacental Passage of Fetal Red Cells in Abortion; Increased Incidence After Curettage and Effect of Oxytocic Drugs

Evaluation of FMH QuikQuant for the detection and quantification of fetomaternal hemorrhage

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