Demystification of enigma on antigen-presenting cell features of human basophils: data from secondary lymphoid organs

Stephen-Victor, Emmanuel; Das, Mrinmoy; Sharma, Meenu; Galeotti, Caroline; Fohrer-Ting, Hélène; Sendid, Boualem; Darnige, Luc; Terris, Benoı̂t; Badoual, Cécile; Bruneval, Patrick; Kaveri, Srini V.; Bayry, Jagadeesh

doi:10.3324/haematol.2016.163451

Cited by 13 publications

(13 citation statements)

References 15 publications

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“…29,30 Also, compared with basophils from mice, human basophils display distinct features. 8,31,32 Therefore the effect of IVIG on basophil function is far from clear. Notably, data from human subject raise an alternative paradigm that IVIG might modulate basophil functions directly rather than the indirect IL-33-dependent pathway.…”

Section: Discussionmentioning

confidence: 99%

Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE

Galeotti

Stephen-Victor

Karnam

et al. 2019

Journal of Allergy and Clinical Immunology

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No Activation Activation Intravenous immunoglobulin (IVIG) activates human basophils through direct interaction with surface-bound IgE, and by IL-3-and Syk-dependent mechanisms Background: Therapeutic normal IgG or intravenous immunoglobulin (IVIG) exerts anti-inflammatory effects through several mutually nonexclusive mechanisms. Recent data in mouse models of autoimmune disease suggest that IVIG induces IL-4 in basophils by enhancing IL-33 in SIGN-related 1-positive innate cells. However, translational insight on these data is lacking. Objective: We sought to investigate the effect of IVIG on human basophil functions. Methods: Isolated circulating basophils from healthy donors were cultured in the presence of IL-3, IL-33, GM-CSF, thymic stromal lymphopoietin, or IL-25. The effect of IVIG and F(ab') 2 and Fc IVIG fragments was examined based on expression of

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Section: Discussionmentioning

confidence: 99%

Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE

Galeotti

Stephen-Victor

Karnam

et al. 2019

Journal of Allergy and Clinical Immunology

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“…56,93,97,98 Nevertheless, human basophils reportedly did not induce antigen-specific T-cell proliferation. [98][99][100] It remains to be investigated whether human basophils can acquire pepti-de2HLA-DR complexes from dendritic cells through trogocytosis and function as APCs.…”

Section: A Controversial Issue: Do Basophils Function As Antigen-presmentioning

confidence: 99%

Multifaceted roles of basophils in health and disease

Karasuyama

Miyake

Yoshikawa

et al. 2018

Journal of Allergy and Clinical Immunology

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Until recently, basophils had often been neglected in immunologic studies because of their minority status among immune cells or confused with tissue-resident mast cells because of some phenotypic similarities between them in spite of different anatomic localization. It is now appreciated that basophils and mast cells are distinct cell lineages and that basophils play important and nonredundant roles distinct from those played by mast cells. On the one hand, basophils contribute beneficially to protective immunity, particularly against parasitic infections. On the other hand, basophils are involved in the development of various disorders, including allergy and autoimmune disease. Basophils interact with other immune cells and nonhematopoietic cells through cell-to-cell contact or basophil-derived factors, such as cytokines and proteases, contributing to the regulation of immune and allergic responses. In this review article we highlight recent advances in our understanding of basophil pathophysiology in human subjects and animal models by consolidating research findings reported during the past 5 years. Further studies on basophils and their products will help identify suitable targets for novel therapeutics in allergy and effective vaccines against parasitic infection.

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“…Upon adoptive transfer, T cell-derived IL-3 also induced basophilia in nematode infection model [32]. IL-3 also primes basophil activation and is the major stimuli for the secretion of IL-13 from the basophils [34,[38][39][40][41]. Although murine basophils have been shown to be activated by IL-33, TSLP and IL-25 [31], various reports from human show that IL-33, IL-25 and TSLP induce only marginal activation of human basophils [18,42].…”

Section: Discussionmentioning

confidence: 99%

Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE

Galeotti

Karnam

Das

et al. 2020

IJMS

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Basophils are rare granulocytes and dysregulated functions of these cells are associated with several atopic and non-atopic allergic diseases of skin, respiratory system and gastrointestinal tract. Both cytokines and immunoglobulin E (IgE) are implicated in mediating the basophil activation and pathogenesis of these disorders. Several reports have shown that healthy individuals, and patients with allergic disorders display IgG autoantibodies to IgE and hence functional characterization of these anti-IgE IgG autoantibodies is critical. In general, anti-IgE IgG autoantibodies modulate basophil activation irrespective of allergen specificity by interacting with constant domains of IgE. Therefore, an ideal solution to prove the functions of such anti-IgE IgG autoantibodies would be to completely eliminate type I high affinity immunoglobulin E receptor (FcɛRI)-bound IgE from the surface of basophils and to demonstrate in an unequivocal manner the role of anti-IgE IgG autoantibodies. In line with previous reports, our data show that FcɛRI on peripheral blood basophils are almost saturated with IgE. Further, acetic acid buffer (pH 4) efficiently removes these FcɛRI-bound IgE. Although immediately following acetic acid-elution of IgE had no repercussion on the viability of basophils, following 24 h culture with interleukin-3 (IL-3), the viability and yield of basophils were drastically reduced in acid-treated cells and had repercussion on the induction of activation markers. Lactic acid treatment on the other hand though had no adverse effects on the viability of basophils and IL-3-induced activation, it removed only a small fraction of the cell surface bound IgE. Thus, our results show that acid buffers could be used for the elution of FcɛRI-bound IgE on the basophil surface for the biochemical characterization of IgE antibodies or for the immediate use of basophils to determine their sensitivity to undergo degranulation by specific allergens. However, these methods are not utile for the functional assays of basophils that require longer duration of culture and entire removal of surface IgE to validate the role of anti-IgE IgG autoantibodies that interact with FcɛRI-bound IgE irrespective of allergen specificity.

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Demystification of enigma on antigen-presenting cell features of human basophils: data from secondary lymphoid organs

Cited by 13 publications

References 15 publications

Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE

Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE

Multifaceted roles of basophils in health and disease

Acid Stripping of Surface IgE Antibodies Bound to FcεRI Is Unsuitable for the Functional Assays That Require Long-Term Culture of Basophils and Entire Removal of Surface IgE

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