Denaturation of Egg Albumin by Pressure

Grant, Earl; Dow, R.B.; Franks, W. R.

doi:10.1126/science.94.2452.616

Cited by 25 publications

(6 citation statements)

References 4 publications

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“…have been observed by many to cause precipitation of protein~.~-5. 8 That addit,ives such as NaCl and glucose counteract the lethal effects of high pressure seeins to indicate that they inay forin coinpIexes with the charged groups and thereby competitively prevent the formation of ionic bonds between protein nioIecules whereby denaturation of the protein is counteracted.…”

Section: Suggested Mechanism Of Protein Denaturation At Very High Prementioning

confidence: 98%

Resistance of microorganisms to hydrostatic pressure

Timson

Short

1965

Biotech & Bioengineering

118

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SummaryA small fraction, about 0.05'%, of the microorganisms in milk are resistant to pressure to at least 150,000 p.s.i. The pressure-resistant microorganisms were shown to be mainly bacterial spores. Some of the pressure-resistant organisms were identified. The lethal effect of pressure is more pronounced in the solid phase than in the liquid phase of water. B. sitbtilis was found to be capable of surviving solid-phase transitions from Ice 11, 111, and 1 ' to Ice I. The lethal effect of high pressure in the liquid phase of water was found not to be counteracted by an increase in temperature. The pressure resistance of B. subtilis was found to be maximum around neutral or slightly basir pH. The damaging effect of low and high pH are counteracted by sodiilm chloride and glucose. It is suggested that the primary mechanism oE protein denaturation by pressures above 30,000 p.s.i. is ionization and formation of ionic bonds between charged groups on proteins, altering their solubility.

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Section: Suggested Mechanism Of Protein Denaturation At Very High Prementioning

confidence: 98%

Resistance of microorganisms to hydrostatic pressure

Timson

Short

1965

Biotech & Bioengineering

118

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“…Complete inactivation occurs at pressures of about 6000 kilos per square centimeter, and coagulation at pressures of about 10,000 kilos per square centimeter. Pressure denaturation of egg albumin results in the liberation of sulfhydryl groups (156); that of tobacco mosaic virus in inactivation and disintegration (222). (c) Freezing: Several qualitative observations on the denaturation of proteins by freezing have been recorded and reviewed by Nord (301).…”

Section: Denaturing Agentsmentioning

confidence: 99%

The Chemistry of Protein Denaturation.

Neurath¹,

Greenstein²,

Putnam³

et al. 1944

Chem. Rev.

331

106

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“…In 1914, Bridgman studied the coagulation of albumen under the pressure (71). However, Grant explained this phenomenon as the protein denaturation phenomenon in 1941 (72). HHP is known to denature proteins and it does not affect covalent bonds, which signifies that the primary and secondary structures of proteins are preserved while the tertiary and quaternary structures are changed (73).…”

Section: Effects Of High Hydrostatic Pressure On Proteinmentioning

confidence: 99%

Research and application of hydrostatic high pressure in tumor vaccines (Review)

Yan

Lin

et al. 2021

Oncol Rep

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It is well known that hydrostatic pressure (HP) is a physical parameter that is now regarded as an important variable for life. High hydrostatic pressure (HHP) technology has influenced biological systems for more than 100 years. Food and bioscience researchers have shown great interest in HHP technology over the past few decades. The development of knowledge related to this area can better facilitate the application of HHP in the life sciences. Furthermore, new applications for HHP may come from these current studies, particularly in tumor vaccines. Currently, cancer recurrence and metastasis continue to pose a serious threat to human health. The limited efficacy of conventional treatments has led to the need for breakthroughs in immunotherapy and other related areas. Research into tumor vaccines is providing new insights for cancer treatment. The purpose of this review is to present the main findings reported thus far in the relevant scientific literature, focusing on knowledge related to HHP technology and tumor vaccines, and to demonstrate the potential of applying HHP technology to tumor vaccine development.

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Denaturation of Egg Albumin by Pressure

Cited by 25 publications

References 4 publications

Resistance of microorganisms to hydrostatic pressure

Resistance of microorganisms to hydrostatic pressure

The Chemistry of Protein Denaturation.

Research and application of hydrostatic high pressure in tumor vaccines (Review)

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