Denatured virion protein 1 of equine rhinitis B virus 1 contains authentic B-cell epitopes recognised in an enzyme-linked immunosorbent assay—Short communication

Abstract

Equine rhinitis B virus 1 (ERBV1), genus Erbovirus, family Picornaviridae, is a pathogen of horses which causes clinical and subclinical infection of the upper respiratory tract in horses. The virus is widespread in European horse populations and the current standard method for the detection of antibody against ERBV1 is by virus neutralisation (VN). VN tests, however, are labour-intensive and time-consuming, require tissue culture facilities, and generally do not provide same-day results. In this study, a protocol for the high-level expression and purification of recombinant virion protein 1 (rVP1) was established using metal-chelate affinity chromatography under denaturing condition. When used as a coating antigen in a prototype enzyme-linked immunosorbent assay (ELISA), denatured rVP1 was recognised by ERBV1 antibody present in horse serum. This finding suggests that denatured rVP1 is a promising candidate for the development of an ELISA to be used in the routine laboratory diagnosis of ERBV1 infection in horses.

Seroprevalence study ofEquine rhinitis B virus(ERBV) in Australian weanling horses using serotype-specific ERBV enzyme-linked immunosorbent assays

“…An ERBV enzyme-linked immunosorbent assay (ELISA) has been reported elsewhere that uses the denatured VP1 protein as an antigen; however, this assay was developed only for the detection of ERBV-1. 11 In the current study, a serotyping ERBV antibodydetection ELISA was developed and used to examine the seroprevalence of ERBV antibodies in a group of 50 weanling horses from 2 farms in a key horse breeding region in New South Wales, Australia. The methodology employed in the ELISAs was similar to that described previously.…”

“…An ERBV enzyme-linked immunosorbent assay (ELISA) has been reported elsewhere that uses the denatured VP1 protein as an antigen; however, this assay was developed only for the detection of ERBV-1. 11…”