Dendrigraft polylysine coated‐poly(glycolic acid) fibrous scaffolds for hippocampal neurons

Abstract

Poly(glycolic acid) (PGA) fibers are a good candidate material for nerve cell scaffolds, which is applicable to the treatment of peripheral nerve injuries. Polylysine is widely used as a coating material for cell substrates to promote nerve cell adhesion. In this study, linear and dendrigraft polylysines were used to coat PGA fibers. The association of large dendrigraft polylysines with PGA fibers was lower and unstable, compared with linear polylysine. However, more hippocampal neurons adhered to PGA fibers coated with large dendrigraft polylysine than linear polylysine. Enhanced cell adhesion was observed, even when the dendrigraft polylysine was coated on the PGA fibers at a low concentration (0.05 μg/mL) or when it was coated in water instead of alkaline buffer. Differences in cell adhesion properties were seen between the dendrigraft polylysine coating and a laminin coating. Thus, large dendrigraft polylysines are a useful coating material for nerve cell scaffolds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2744-2750, 2016.

Digitizing Poly-l-lysine Dendrigrafts: From Experimental Data to Molecular Dynamics Simulations

“…In contrast to PAMAM and PEI, DGLs are biodegradable, 2 exhibit low cellular toxicities, 3 and turned out to be non-immunogenic. 4 As a consequence, DGLs have recently gained a huge interest in the biomedical field during the last quinquennium, with numerous applications in drug and gene delivery, 2,3,[5][6][7][8][9][10][11][12][13][14][15][16][17][18] biomaterials and tissue engineering, [19][20][21][22][23][24][25] bio-imaging [26][27][28][29][30][31] and biosensing. [32][33][34] Despite this growing use of DGLs, a deeper understanding of the molecular level properties of these macromolecules is missing.…”

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

“…In an interesting example, Kojima et al coated PGA fibres with linear and dendrigraft polylysines to increase cell attachment to them. They found an increased cell adhesion to the PGA fibres coated with dendrigraft polylysine even at a low concentration (0.05 lg/ml) [97].…”

Everything You Always Wanted to Know about Poly‐l‐lysine Dendrigrafts (But Were Afraid to Ask)

“…[51] In addition, DGL G4,i na ssociation with ap oly(e-caprolactone) membrane, was also an effective nanoreservoir for the nerve growth factor;t he resulting biomaterial being able to induce the full vascularization and innervation of atooth in vivo. In the context of regenerative medicine for peripheral nerve,D GLs were identified to be superiort ol inear poly-l-lysines of similar molecular weights in promoting nerve cell adhesion, suggesting that nerve cells adhered more efficiently to the dendritic-coated poly(glycolica cid) fibres.…”

“…In the context of regenerative medicine for peripheral nerve,D GLs were identified to be superiort ol inear poly-l-lysines of similar molecular weights in promoting nerve cell adhesion, suggesting that nerve cells adhered more efficiently to the dendritic-coated poly(glycolica cid) fibres. [51] In addition, DGL G4,i na ssociation with ap oly(e-caprolactone) membrane, was also an effective nanoreservoir for the nerve growth factor;t he resulting biomaterial being able to induce the full vascularization and innervation of atooth in vivo. [52] Biosensing In ab iosensing purpose,athird-generation generation DGL G3,i mmobilizedo np lasma activated polypropylene filters, was reported to be an efficient capture agentf or concentrating bacteriophage MS2, allowingt he quantification of the model virus in large volumes of water.…”

“…Tissue engineering aims at the regeneration of damaged or dysfunctional tissues and organs through the use of polymeric scaffolds that promote cell adhesion and/or deliver growth factors. In the context of regenerative medicine for peripheral nerve, DGLs were identified to be superior to linear poly‐ l ‐lysines of similar molecular weights in promoting nerve cell adhesion, suggesting that nerve cells adhered more efficiently to the dendritic‐coated poly(glycolic acid) fibres . In addition, DGL G4 , in association with a poly(ϵ‐caprolactone) membrane, was also an effective nanoreservoir for the nerve growth factor; the resulting biomaterial being able to induce the full vascularization and innervation of a tooth in vivo …”