Dendrimer-coated magnetic particles for radionuclide separation

Grüttner, Cordula; Böhmer, Volker; Casnati, Alessandro; Dozol, Jean‐François; Reinhoudt, David N.; Reinoso-García, Marta M.; Rudershausen, Sandra; Teller, Joachim; Ungaro, Rocco; Verboom, Willem; Wang, Pingshan

doi:10.1016/j.jmmm.2005.01.073

Cited by 31 publications

(21 citation statements)

References 14 publications

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“…Ginet et al functionalized lipid-coated magnetic nanoparticles with a phosphohydrolase for the 6 degradation of the pesticide ethyl-paraoxon; the kinetic properties were comparable to the free enzyme [21]. Dendrimer-modified and plain magnetite nanoparticles (MNPs) have been extensively studied for their potential application in environmental decontamination, medicine and biology [22][23][24][25]. For example, MNP coated with receptor proteins or other functional molecules have found application in imaging and therapy [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

“…For example, MNP coated with receptor proteins or other functional molecules have found application in imaging and therapy [26][27][28]. Grüttner et al reported MNPs functionalized with chelating groups on the surface to bind lanthanides and actinides in order to remove radionuclides from nuclear wastes [24]. Magnetically assisted processes have been reported to purify water [25] and MNPs have been proven useful in protein immobilization and purification and DNA extraction [23,29].…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Daumann

Larrabee

Ollis

et al. 2014

Journal of Inorganic Biochemistry

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Annually thousands of people die or suffer from organophosphate (pesticide) poisoning. In order to remove these toxic compounds from the environment, the use of enzymes as bioremediators has been proposed. We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes. The mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on PAMAM dendrimer-modified magnetite nanoparticles. The immobilized system was characterized using elemental analysis as well as infrared, transmission electron and X-ray photoelectron spectroscopies. The amount of GpdQ that was immobilized with the optimized procedure was 1.488 nmol per g MNP. A kinetic assay has been designed to evaluate the activity of the system towards organophosphoester substrates. The specific activity towards BPNPP directly after immobilization was 3.55 μmol mg -1 min -1 , after one week 3.39 μmol mg -1 min -1 and after 120 days 3.36 μmol mg -1 min -1 , demonstrating that the immobilized enzyme was active for multiple cycles and could be stored on the nanoparticles for a prolonged period.3

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Daumann

Larrabee

Ollis

et al. 2014

Journal of Inorganic Biochemistry

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“…Dendrimer modified and plain magnetite nanoparticles (MNP) have been extensively studied for their potential application in environmental decontamination, medicine and biology [1][2][3][4]. For example, Grüttner et al reported MNP functionalized with chelating groups on the surface to bind lanthanides and actinides in order to remove radionuclides from nuclear wastes [3].…”

Section: Strategies To Immobilize Enzymes and Catalystsmentioning

confidence: 99%

“…For example, Grüttner et al reported MNP functionalized with chelating groups on the surface to bind lanthanides and actinides in order to remove radionuclides from nuclear wastes [3]. MNPs also have potential in transport of pharmaceuticals by coating them with, for example, streptavidin (SA) which displays an extremely high affinity for biotin [2].…”

Section: Strategies To Immobilize Enzymes and Catalystsmentioning

confidence: 99%

Immobilization of GpdQ and Related Models for Bioremedial Applications

Daumann

2014

Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases and Their Biomimetic Complexes

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“…Como se trata de um campo de pesquisa totalmente aberto, espera-se que despertará maior interesse da comunidade em futuras explorações. Ressalta-se ainda que as determinações energéticas das interações ácido/base, na interface sólido líquido, despontam como um aspecto de relevância nas possí-veis separações envolvendo mistura de cátions 126,127 .…”

Section: Conclusãounclassified

A relevante potencialidade dos centros básicos nitrogenados disponíveis em polímeros inorgânicos e biopolímeros na remoção catiônica

Airoldi¹

2008

Quím. Nova

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cation removal by using nitrogenated basic centers. The data demonstrate the importance of the desired groups when free or immobilized on natural or synthesized inorganic polymers through silanol groups. Thus, the most studied silica gel is followed by natural crysotile and talc polymers, and the synthesized mesopore silicas, talc-like, silicic acids, phosphates and phyllosilicates. The organic natural biopolymeric chitin and cellulose were chemically modified to improve the availability of the amine groups or the reactivity with desirable molecules to enlarge the content of basic centers. The cation removal takes place at the solid/liquid interface and some interactive effects have their thermodynamic data determined.Keywords: inorganic polymer; biopolymer; cation removal. INTRODUÇÃONos dias de hoje, quando se veicula assuntos relacionados com superfícies, principalmente na área de Química Inorgânica, os interessados se voltam mais diretamente à rotulação dos profissionais atuantes como químicos de materiais. Porém, muitas vezes são esquecidos os aspectos envolvendo as ligações efetuadas entre os átomos, que estão em quantidade maiores ou menores na formação do esqueleto polimérico. Mesmo sem o conhecimento de tais detalhes e ainda sobre a estrutura é possível empregar o material em atividades tanto acadêmicas como tecnológicas, sendo comumente destacada a propriedade da adsorção. Curiosamente, o material derivado da queima ou extraído de minas naturais, após tratamento, deriva o chamado carvão ativado, o qual foi o primeiro material usado para a remoção de metais pesados contidos em efluentes industriais. No entanto, em qualquer processo operacional, o custo envolvido precisa ser examinado para que seja viável o uso, pois o preço cresce em função das especificidades requeridas e, de fato, a purificação é bastante relevante, o que chega a inviabilizá-lo na adsorção, mas que possibilita o emprego mais amplo somente em pequenas escalas laboratoriais 1 . Diante dos aspectos econômicos limitantes na obtenção do carvão de alta pureza, as pesquisas foram paulatinamente direcionadas para outras fontes alternativas. Um ponto marcante neste estudo foi buscar materiais naturais de baixo custo, que pudessem ter alta capacidade de adsorção, o que implicava na existência de centros quimicamente ativos na superfície, para que pudessem atuar com alta eficiência na retenção de cátions indesejáveis existentes em um leito aquoso contaminado. Nesta direção, o progresso do campo passou a ser mais eficaz após o conhecimento das funções quí-micas disponíveis nas superfícies dos materiais e possíveis tipos de ligações que poderiam formar frente a um determinado soluto, no decorrer do processo de adsorção na interface sólido/líquido 2 . Uma maneira de se obter o material com baixo custo é explorar fontes amplamente encontradas na natureza, que exijam operações simples para chegar a um composto final de composição definida e de alta pureza, fato que nem sempre é alcançado. Dentre uma série de materiais, os zeólitos se ajustam com essas...

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Dendrimer-coated magnetic particles for radionuclide separation

Cited by 31 publications

References 14 publications

Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation

Immobilization of GpdQ and Related Models for Bioremedial Applications

A relevante potencialidade dos centros básicos nitrogenados disponíveis em polímeros inorgânicos e biopolímeros na remoção catiônica

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