Dendrimer Modified SWCNTs for High Efficient Delivery and Intracellular Imaging of Survivin SiRNA

Abstract

Herein we reported that polyamidoamine dendrimer (PAMAM) modified single walled carbon nanotubes (PSWCNTs) were successfully used for high efficient delivery and intracellular imaging of survivin siRNA vector. No.4 generation of PAMAM dendrimers were used to modify single walled carbon nanotubes, and characterized by high resolution transmission electron microscopy (HR-TEM), and atom force microscopy (AFM), survivin shRNA vectors were constructed and identified, and then dendrimer-modified SWCNTs were mixed with survivin shRNA vectors, resultant dendrimer-SWCNTs-survivin shRNA vector mixtures co-cultured with human gastric cancer MGC803 cells, then the expression of survivin shRNA vectors in MGC803 cells was observed by fluorescent microscopy, cell viability was analyzed by MTT method. Results showed that survivin shRNA vector was successfully expressed in MGC803 cells, and MGC 803 cell growth was markedly inhibited. In conclusion, dendrimer (PAMAM) modified single walled carbon nanotubes can be used for high efficient delivery and intracellular imaging of siRNA, and own great potential in applications such as gene or drug delivery and tumor targeted imaging and simultaneous therapy in near future.

RGD-conjugated silica-coated gold nanorods on the surface of carbon nanotubes for targeted photoacoustic imaging of gastric cancer

“…Gold nanorods (GNRs), because of their small size, strong light-enhanced absorption in the NIR, and plasmon resonance-enhanced properties, have become attractive noble nanomaterials for their potential in applications such as photothermal therapy [ 37 ], biosensing [ 38 ], PA imaging [ 39 ], and gene delivery [ 40 ] for cancer treatment. However, the toxicity derived from a large amount of the surfactant cetyltrimethylammonium bromide (CTAB) during GNR synthesis severely limits their biomedical applications.…”

Modification of Carbon Nanotubes as an Effective Solution for Cancer Therapy

“…Then, modified Gold nanorods peptides with the amino silane agent were conjugated with the sGNR-MWCNT hybrid structure in a struggle to eliminate their cytotoxicity and improving their biocompatibility, as a result of which RGD-conjugated sGNR-MWNT (RGD-GNR-MWNT) nano-probes were used for photoacoustic imaging of in vivo gastric MGC803 and GES-1 cells. Gold nano-rods, due to their small size, strong light-enhanced absorption in the NIR, and plasmon resonance-enhanced properties have become attractive noble nano-materials for their potential in applications including, photothermal therapy [162], bio-sensing [163], PA imaging [164], and gene delivery [165] for cancer treatment. According to the cell viability data RGD-GNR-MWNT nanoprobes revealed good biocompatibility to MGC803 and GES-1 cells within the dose of 800 µg/mL in the medium.…”