Dendrimeric Alkylated Polyethylenimine Nano-carriers with Acid-Cleavable Outer Cationic Shells Mediate Improved Transfection Efficiency Without Increasing Toxicity

Steele, Terry W. J.; Shier, W. Thomas

doi:10.1007/s11095-010-0058-1

Cited by 23 publications

(11 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cationic lipids are among the most widely used non-viral gene delivery vectors [5-12]. Cationic lipids are amphipathic molecules that are composed of a hydrophobic part and a cationic head group which may be attached to the hydrophobic part directly or through a linker [13,14].…”

Section: Introductionmentioning

confidence: 99%

Efficient Gene Silencing by Self-Assembled Complexes of siRNA and Symmetrical Fatty Acid Amides of Spermine

2011

View full text Add to dashboard Cite

Gene silencing by siRNA (synthetic dsRNA of 21-25 nucleotides) is a well established biological tool in gene expression studies and has a promising therapeutic potential for difficult-to-treat diseases. Five fatty acids of various chain length and oxidation state (C12:0, C18:0, C18:1, C18:2, C22:1) were conjugated to the naturally occurring polyamine, spermine, and evaluated for siRNA delivery and gene knock-down. siRNA delivery could not be related directly to gene silencing efficiency as N4,N9-dierucoyl spermine resulted in higher siRNA delivery compared to N4,N9-dioleoyl spermine. GFP silencing in HeLa cells showed that the unsaturated fatty acid amides are more efficient than saturated fatty acid amides, with N4,N9-dioleoyl spermine resulting in the most efficient gene silencing in the presence of serum. The alamarBlue cell viability assay showed that fatty acid amides of spermine have good viability (75%–85% compared to control) except N4,N9-dilauroyl spermine which resulted in low cell viability. These results prove that unsaturated fatty acid amides of spermine are efficient, non-toxic, non-viral vectors for siRNA mediated gene silencing.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficient Gene Silencing by Self-Assembled Complexes of siRNA and Symmetrical Fatty Acid Amides of Spermine

2011

View full text Add to dashboard Cite

show abstract

“…The maleamic acid from the previous step was cyclised by dehydration with acetic anhydride and sodium acetate as described in the literature [15,16]. Briefly, 7 mmol of the obtained maleamic acid was mixed with 0.35 g of anhydrous sodium acetate and 10 mL of acetic anhydride.…”

Section: Trans-4-nn′-dimethylamino-4′-stilbene Maleimide (2a)mentioning

confidence: 99%

Novel Photochrome Aptamer Switch Assay (PHASA) for Adaptive Binding to Aptamers

Papper

Pokholenko

et al. 2014

J Fluoresc

Self Cite

View full text Add to dashboard Cite

A novel Photochrome-Aptamer Switch Assay (PHASA) for the detection and quantification of small environmentally important molecules such as toxins, explosives, drugs and pollutants, which are difficult to detect using antibodies-based assays with high sensitivity and specificity, has been developed. The assay is based on the conjugation of a particular stilbene-analyte derivative to any aptamer of interest. A unique feature of the stilbene molecule is its reporting power via trans-cis photoisomerisation (from fluorescent trans-isomer to non-fluorescent cis-isomer) upon irradiation with the excitation light. The resulting fluorescence decay rate for the trans-isomer of the stilbene-analyte depends on viscosity and spatial freedom to rotate in the surrounding medium and can be used to indicate the presence of the analyte. Quantification of the assay is achieved by calibration of the fluorescence decay rate for the amount of the tested analyte. Two different formats of PHASA have been recently developed: direct conjugation and adaptive binding. New stilbene-maleimide derivatives used in the adaptive binding format have been prepared and characterised. They demonstrate effective binding to the model thiol compound and to the thiolated Malachite Green aptamer.

show abstract

“…Their side chains will be neutralized and peel off when pH gets down. At the same time, the noncharged hydrophobic side chains will insert into the hydrophobic part of membrane and decrease the stability of endosome, which is helpful for enhancing gene transfection (Lackey et al, 1999;Cheung et al, 2001;Steele and Shier, 2010) (as shown in Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

Biocompatible anionic polyelectrolyte for improved liposome based gene transfection

Chen

Zeng

et al. 2015

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Dendrimeric Alkylated Polyethylenimine Nano-carriers with Acid-Cleavable Outer Cationic Shells Mediate Improved Transfection Efficiency Without Increasing Toxicity

Abstract: Using acid-cleavable linkers to attach an outer shell of short, highly-charged oligocations to a PEI-based dendrimeric vector substantially increased transfection efficiency without increasing cytotoxicity.

Cited by 23 publications

References 45 publications

Efficient Gene Silencing by Self-Assembled Complexes of siRNA and Symmetrical Fatty Acid Amides of Spermine

Efficient Gene Silencing by Self-Assembled Complexes of siRNA and Symmetrical Fatty Acid Amides of Spermine

Novel Photochrome Aptamer Switch Assay (PHASA) for Adaptive Binding to Aptamers

Biocompatible anionic polyelectrolyte for improved liposome based gene transfection

Contact Info

Product

Resources

About