Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia; Gammella, Mariela; Digiacomo, Sebastian; Torre, Beatriz G. de la; Andreu, David; Montoya, María; Sánchez‐Madrid, Francisco; Blanco, Esther; Zamorano, Patricia

doi:10.1371/journal.pone.0185184

Cited by 21 publications

(15 citation statements)

References 37 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Attempts to use B 2 T and B 4 T dendrimer peptides as vaccines in cattle showed a trend toward a reduced capacity to confer protection relative to swine, including the need for a third immunization to elicit protective levels on neutralizing antibodies [39,40]. Failure in conferring protection in cattle has been described for linear FMDV peptide vaccines containing a heterologous T cell epitope [41].…”

Section: Discussionmentioning

confidence: 99%

A Single Dose of Dendrimer B2T Peptide Vaccine Partially Protects Pigs against Foot-and-Mouth Disease Virus Infection

et al. 2020

Self Cite

View full text Add to dashboard Cite

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals whose control relies on efficient vaccination. We have reported that dendrimer peptide B2T, with two copies of FMDV B-cell epitope VP1 (136–154) linked through maleimide units to T-cell epitope 3A (21–35)], elicits potent B- and T-cell specific responses and confers solid protection in pigs to type-O FMDV challenge after two doses of peptide. Herein we now show that B2T evokes specific protective immune responses after administration of a single dose of either 2 or 0.5 mg of peptide. High titers of ELISA and neutralizing antibodies against FMDV were detectable at day 15 post-immunization. Likewise, activated T cells and induced IFN-γ response to in vitro recall with FMDV peptides were also detected by the same day. Further, in 70% of B2T-vaccinated pigs, full protection—no clinical signs of disease—was observed upon virus challenge at day 25 post-immunization. These results strengthen the potential of B2T as a safe, cost-effective candidate vaccine conferring adequate protection against FMDV with a single dose. The finding is particularly relevant to emergency scenarios permitting only a single shot immunization.

show abstract

Section: Discussionmentioning

confidence: 99%

A Single Dose of Dendrimer B2T Peptide Vaccine Partially Protects Pigs against Foot-and-Mouth Disease Virus Infection

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The dendrimeric peptides reproduced the B-cell (PVTNVRGDLQVLAQKAART, residues 136–154 of VP1) and T-cell (AAIEFFEGMVHDSIK, residues 21–35 of 3A) epitopes of FMDV O-UKG 11/01 ( Figure 1 ). As detailed in [ 19 ], B 2 T and B 4 T constructions were assembled by conjugation of a T-epitope N terminally elongated with Lys residues providing 2 or 4 levels of branching and functionalized with chloroacetyl units and an N-acetylated B epitope with a C-terminal Cys whose thiol group reacts with the chloroacetyl group to give a thioether link. Additional details on the synthesis are available in previously published works [ 11 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies indicate that vaccination with dendrimeric peptides based on the amino acid sequence of 3A (T-cell epitope) and VP1 GH loop (B-cell epitope) from the type O FMDV O/UKG/11/2001, and branched by means of thioether or maleimide conjugation chemistries, elicits an immune response that achieved protection in up to 100% of the vaccinated pigs [ 16 ]. Likewise, we recently reported that similar dendrimeric peptides, based on the amino acid sequences from the type O FMDV O1/Campos/Bra/58, including a VP4 sequence as T-cell epitope, can protect cattle against homologous challenge [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle

Soria

Quattrocchi

Langellotti

et al. 2018

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B2T and B4T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B2T and B4T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point.

show abstract

“…Protection showed limited correlation with serum neutralization activity and lymphoproliferation in response to whole virus (48). Moreover, multiple antigenic peptides or dendrimers (radial or wedge-like branched macromolecules with a peptide core attached to a defined number of epitopes), displaying one copy of a FMDV T-cell epitope branching into several copies of a B-cell epitope, fully protected swine and cattle (7,46). Recent studies using three synthetic peptides vaccines against the A/HuBWH/CHA/2009 strain of FMDV of 59 to 87 amino acids in size, based on immunogenic epitopes in the VP1, 3A, and 3D proteins conferring 60% protection in cattle after a single immunization (56).…”

mentioning

confidence: 99%

Recent progress in vaccines against foot-and-mouth disease

Niedbalski¹,

Fitzner²,

Bulenger³

2019

Medycyna Weterynaryjna

View full text Add to dashboard Cite

Foot-and-mouth disease (FMD) is the most highly contagious disease affecting livestock resulting in a significant adverse economic impact worldwide. Disease outbreaks in previously FMD-free countries are initially controlled by the culling of infected and in-contact animals, restriction of susceptible animal movement, and vaccination with an inactivated whole-virus antigen preparation. With increasing trade, commerce globalization, and people migration, it is likely that more inter-pool viral exchanges and spillovers will occur posing a greater threat to both, endemic and non-endemic regions, mostly due to the limited antigenic coverage of current vaccines. Currently available inactivated FMD vaccines have a number of disadvantages, including incomplete inactivation of the virus, they require multiple vaccination to maintain good levels of immunity and periodic inclusion of new viral strains into the vaccine formulation to cover new FMDV subtypes against which existing vaccines no longer protect and lack differentiation of infected from vaccinated animals (DIVA). The essential aim of DIVA strategy is realization of the so-called “vaccinate-to-live” policy, which is based on the principles that vaccinated animals exposed to FMDV will not transmit the virus. To address the shortcomings of inactivated vaccines, many efforts are currently devoted to developing novel FMD vaccines, including attenuated and marker inactivated vaccines, recombinant protein vaccines, synthetic peptide vaccines and empty capsid vaccines. Novel vaccine platforms offer promising alternatives for effective FMD control. It is likely that in the near future, multiple FMD vaccine approaches will compete for diverse markets, providing fit-for purpose solutions to evolving challenges in preventing and controlling FMD worldwide.

show abstract

Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Cited by 21 publications

References 37 publications

A Single Dose of Dendrimer B2T Peptide Vaccine Partially Protects Pigs against Foot-and-Mouth Disease Virus Infection

A Single Dose of Dendrimer B2T Peptide Vaccine Partially Protects Pigs against Foot-and-Mouth Disease Virus Infection

Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle

Recent progress in vaccines against foot-and-mouth disease

Contact Info

Product

Resources

About