Dendrislides, dendrichips: a simple chemical functionalization of glass slides with phosphorus dendrimers as an effective means for the preparation of biochips

Trévisiol, Emmanuelle; Berre-Anton, Véronique Le; Leclaire, Julien; Pratviel, Geneviève; Caminade, Anne‐Marie; Majoral, Jean‐Pierre; François, Jean; Meunier, Bernard

doi:10.1039/b307928g

Cited by 84 publications

(68 citation statements)

References 38 publications

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“…2B) than with electrostatic slides (Figure 3A). This result is consistent with our previous report that the functionalization of surface with dendrimers reduces the non specific adsorption of fluorescent material [8]. In addition, the "donut" formation of spots frequently obtained after deposition of DNA molecules by contact spotting was no longer observed since the μCP is a "dry" deposition technique.…”

Section: Resultssupporting

confidence: 92%

“…Experiments reported in this paper were carried out using two different type of glass slides that differed by their surface functionalization: positively charged amine glass slides (Ultra Gap, Dow corning) and dendrislides, which are glass slides that have been functionalized with nanometric spherical dendrimeric particles bearing aldehydes reactive group at the periphery for covalent attachment of the 5'-NH 2 probes [7,8]. These two types of functionalized slides were printed for 15 sec with a stamp that has been incubated for 30 sec with a 10 μM solution of 35-mers 5'-NH 2 probe in Na-phosphate buffer at pH 9.0.…”

Section: Resultsmentioning

confidence: 99%

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Direct microcontact printing of oligonucleotides for biochip applications

et al. 2005

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BackgroundA critical step in the fabrication of biochips is the controlled placement of probes molecules on solid surfaces. This is currently performed by sequential deposition of probes on a target surface with split or solid pins. In this article, we present a cost-effective procedure namely microcontact printing using stamps, for a parallel deposition of probes applicable for manufacturing biochips.ResultsContrary to a previous work, we showed that the stamps tailored with an elastomeric poly(dimethylsiloxane) material did not require any surface modification to be able to adsorb oligonucleotides or PCR products. The adsorbed DNA molecules are subsequently printed efficiently on a target surface with high sub-micron resolution. Secondly, we showed that successive stamping is characterized by an exponential decay of the amount of transferred DNA molecules to the surface up the 4th print, then followed by a second regime of transfer that was dependent on the contact time and which resulted in reduced quality of the features. Thus, while consecutive stamping was possible, this procedure turned out to be less reproducible and more time consuming than simply re-inking the stamps between each print. Thirdly, we showed that the hybridization signals on arrays made by microcontact printing were 5 to 10-times higher than those made by conventional spotting methods. Finally, we demonstrated the validity of this microcontact printing method in manufacturing oligonucleotides arrays for mutations recognition in a yeast gene.ConclusionThe microcontact printing can be considered as a new potential technology platform to pattern DNA microarrays that may have significant advantages over the conventional spotting technologies as it is easy to implement, it uses low cost material to make the stamp, and the arrays made by this technology are 10-times more sensitive in term of hybridization signals than those manufactured by conventional spotting technology.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Direct microcontact printing of oligonucleotides for biochip applications

et al. 2005

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“…Les dendrimères phosphorés peuventêtre utilisés pour recouvrir et fonctionnaliser des surfaces afin de permettre l'immobilisation de biomolécules, démontrant trois principaux avantages par rapport aux chimies de fonctionnalisation existantes : augmentation de la densité de sondes par unité de surface, accessibilité de l'échantillon aux sondes avec une hybridation en 3D et non en 2D ; diversité des fonctions réactionnelles portées par les dendrimères qui permettent l'immobilisation de tout un large Berre et al, 2003 ;Trévisiol et al, 2003).…”

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Article retiré – Utilisation de dendrimères pour une nanomédecine innovatrice

Majoral

2013

Biologie Aujourd'hui

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Reçu le 15 octobre 2012Résumé -Les dendrimères et plus particulièrement les dendrimères phosphorés présentent une grande variété d'applications dans de nombreux domaines : biologie, médecine mais aussi science des matériaux ou encore catalyse. Cet article décrit succinctement quelques-unes des applications des dendrimères phosphorés en nanomédecine. Mots clés : Dendrimères / phosphore / nanomédecine / imagerie / applications biologiques Abstract -Using dendrimers for an innovative nanomedicine.Dendrimers and more particularly phosphorus containing dendrimers are useful nanoobjects for a variety of applications ranging from biology, medicine and materials sciences to catalysis. This manuscript reports briefly some applications of phosphorus dendrimers in the field of nanomedicine.

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“…Dendrimer-activated surfaces have been demonstrated for higher immobilization efficiencies and lower detection limits. [28][29][30][31] Here, PAMAM-SAM served to increase the surface density of the reactive aldehyde groups and also acted as a 6.7 nm spacer [27] for easier access of target DNA towards immobilized probe DNA. Figures S1,S2 (Supporting Information) show the preparation scheme of two control surfaces containing ald-SAM patterns surrounded by either a (1H,1H,2H,2H-perfluorooctyl) trichlorosilane (FOTS)-SAM background or a hexamethyldisilazane (HMDS)-SAM background.…”

mentioning

confidence: 99%

“…The Generation-6 (G-6) PAMAM molecule, terminated with 256 primary amine groups and 6.7 nm in diameter, [27] was used to increase the surface density of aldehyde functional groups to increase the oligonucleotide-immobilization efficiency. [28][29][30][31] Current techniques for patterning biomolecules involve the use of polymer-based templates, [32][33][34][35] which can be removed mechanically without the use of organic solvents after biomolecule immobilization. However, serious limitations exist in each case.…”

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confidence: 99%