Dendritic architecture of corticothalamic neurons in a rat model of microgyria

Rocco, F. Di; Giannetti, Stefano; Gaglini, Pierpaolo; Rocco, Concezio Di; Granato, Alberto

doi:10.1007/s00381-002-0682-0

Cited by 8 publications

(2 citation statements)

References 24 publications

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“…We also cannot rule out the possibilities that the increase in mEPSC frequency is caused by an increase in release probability or a redistribution of the same number of excitatory synapses, causing more mEPSCs to be detected. Altered dendritic morphologies shown to occur in this model include longer basal dendrites of layer V neurons (Di Rocco et al 2002), which may specifically be associated with redistribution of excitatory synapses. These dendrites may also provide increased synaptic "space" for hyperinnervation.…”

Section: Hyperinnervation Of Pmg Regionmentioning

confidence: 99%

Excitatory and Inhibitory Postsynaptic Currents in a Rat Model of Epileptogenic Microgyria

Jacobs¹,

Prince²

2005

Journal of Neurophysiology

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Developmental cortical malformations are common in patients with intractable epilepsy; however, mechanisms contributing to this epileptogenesis are currently poorly understood. We previously characterized hyperexcitability in a rat model that mimics the histopathology of human 4-layered microgyria. Here we examined inhibitory and excitatory postsynaptic currents in this model to identify functional alterations that might contribute to epileptogenesis associated with microgyria. We recorded isolated whole cell excitatory postsynaptic currents and GABA(A) receptor-mediated inhibitory currents (EPSCs and IPSCs) from layer V pyramidal neurons in the region previously shown to be epileptogenic (paramicrogyral area) and in homotopic control cortex. Epileptiform-like activity could be evoked in 60% of paramicrogyral (PMG) cells by local stimulation. The peak conductance of both spontaneous and evoked IPSCs was significantly larger in all PMG cells compared with controls. This difference in amplitude was not present after blockade of ionotropic glutamatergic currents or for miniature (m)IPSCs, suggesting that it was due to the excitatory afferent activity driving inhibitory neurons. This conclusion was supported by the finding that glutamate receptor antagonist application resulted in a significantly greater reduction in spontaneous IPSC frequency in one PMG cell group (PMG(E)) compared with control cells. The frequency of both spontaneous and miniature EPSCs was significantly greater in all PMG cells, suggesting that pyramidal neurons adjacent to a microgyrus receive more excitatory input than do those in control cortex. These findings suggest that there is an increase in numbers of functional excitatory synapses on both interneurons and pyramidal cells in the PMG cortex perhaps due to hyperinnervation by cortical afferents originally destined for the microgyrus proper.

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Section: Hyperinnervation Of Pmg Regionmentioning

confidence: 99%

Excitatory and Inhibitory Postsynaptic Currents in a Rat Model of Epileptogenic Microgyria

Jacobs¹,

Prince²

2005

Journal of Neurophysiology

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“…The Golgi staining or “la reazione nera” (the black reaction) discovered by Camillo Golgi in 1873 is one of the best and most elegant methods to observe the morphology of the whole neuron; it still represents the standard for visualization of dendrites and spines, even if only about 1–10% of neurons are stained (Shankaranarayana Rao and Raju, ). Golgi or Golgi‐like methods have been used to study the normal and abnormal neuronal morphology in different species (Jacobs et al ., ; Giannetti et al ., ; Di Rocco et al ., , ; Granato et al ., ; Anderson et al ., ; Butti et al . ; Johnson et al ., ; Bicanic et al ., ).…”

Section: Introductionmentioning

confidence: 98%

The Claustrum of the Pig: An Immunohistochemical and a Quantitative Golgi Study

Pirone

Miragliotta

Cozzi

et al. 2019

The Anatomical Record

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Despite increasing interest in the claustrum (Cl) over the last decades, its function is still a puzzling problem. Among the experimental species of potential use in Cl research, the pig is considered an interesting model, because of the similarities of its brain with the corresponding cortical and subcortical human structures. The swine Cl presents a peculiar morphology, characterized by a wide posterior enlargement, ideal for physiological investigations. There is a wealth of data on general anatomy, cytoarchitecture, and chemo architecture of the Cl, but much less is known about the dendritic morphometry of its neurons. Dendritic length and branching pattern are key features to understand the organization of the microcircuitry, and thus the delineation of the structure-function relationships of the Cl. To this effect, we undertook (a) a quantitative study of the dendrites of the spiny neurons of the swine Cl, employing the Golgi staining; and (b) an immunohistochemical analysis to describe the distribution of the parvalbumin (PV)immunoreactive interneurons throughout the same nucleus. Taken together, the results that we report here show that the dendritic architecture and the distribution of the PV expressing interneurons change when the Cl of this species changes its shape along the rostro-caudal axis, thus suggesting a potentially specific function for the large posterior puddle. Anat Rec, 302:1638-

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Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing and Electrophysiology

Granato

Giorgio

2015

Neuromethods

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Dendritic architecture of corticothalamic neurons in a rat model of microgyria

Cited by 8 publications

References 24 publications

Excitatory and Inhibitory Postsynaptic Currents in a Rat Model of Epileptogenic Microgyria

Excitatory and Inhibitory Postsynaptic Currents in a Rat Model of Epileptogenic Microgyria

The Claustrum of the Pig: An Immunohistochemical and a Quantitative Golgi Study

Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing and Electrophysiology

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