Dendritic branching features of posterodorsal medial amygdala neurons of adult male and female rats: Further data based on the Golgi method

Dall'Oglio, Aline; Gehlen, Günther; Achaval, Matilde; Rasia‐Filho, Alberto A.

doi:10.1016/j.neulet.2007.10.051

Cited by 31 publications

(23 citation statements)

References 31 publications

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“…Histological, connectivity, neurochemical, and functional studies have divided the rat MeA into the anterodorsal (MeAD), anteroventral, posterodorsal (MePD), and posteroventral (MePV) subnuclei [8,9,[15][16][17][18] ; Fig.(1). These subnuclei are reciprocally interconnected in the same hemisphere [15].…”

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

“…Therefore, it is not surprising that plasma gonadal steroids can affect MePD cells in both sexes. As reviewed recently [2], male-female differences in the rat posterior MeA and the MePD specifically include the following: MePD volume [62], neuron and glial cells number [44,46], neuronal somatic volumes [63], spatial orientation of the dendritic branches and dendritic length [17,64], dendritic spine density [40,65], synaptic connectivity and frequency of excitatory post-synaptic currents [20,66,67], and the concentration of neuropeptides and neurotransmitter binding sites [58,[68][69][70][71][72][73][74][75][76]. Investigations of astrocyte density and glial fibrillary acidic protein immunoreactivity have suggested comparable interpretations [44,45,77].…”

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

“…Representative images of these neurons can be found elsewhere [17,18,37,78,79]. There is no clear evidence for the presence of MePD medium spiny stellate neurons, similar to those found in the striatum, in adult Wistar rats [37].…”

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

“…No single morphological characteristic is used to reliably identify these neurons [17,18,37,[78][79][80][81][82][83][84][85] Though gonadal hormone receptors are not present in all cells in the neural regions, the classic Golgi method cannot distinguish neurons that concentrate sex steroids from those that do not [78,81]. However, confocal immunofluorescent microscopy has revealed that transcription factor Lhx6-immunoreactive cells constitute approximately 80% of all neurons in the MePD [82].…”

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

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Morphological and Functional Features of the Sex Steroid-Responsive Posterodorsal Medial Amygdala of Adult Rats

Rasia‐Filho

Haas²,

Oliveira³

et al. 2012

MRMC

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The rat posterodorsal medial amygdala (MePD) expresses receptors for gonadal hormones and integrates sex steroid-sensitive subcortical networks. Male-female differences are found in the morphology, connectivity, and local neuropil structure of MePD. For example, dendritic spine density is sexually-dimorphic and changes with the estrous cycle and following gonadal hormones manipulations. Due to its connectivity, the MePD may affect emotionally-loaded social behaviors, according to a former Newman's seminal proposition. Unilateral fiber-sparing ibotenic acid damage of the MePD does not impair male sexual behavior. However, microinjecting glutamate and histamine into the right MePD facilitates ejaculation. Further, MePD-lesioned rats are not different from normal rats in anxiety-like behavior as evaluated by the elevated plus maze test or innate fear test induced by a live cat. In another study, an adapted model for inducing aggressive behavior in rats by a brief period of restraint prior to the resident-intruder paradigm was used to study Fos-immunoreactivity in the MePD. Following stressful stimulation (restraint) or the restraint and fight condition, but not after aggression alone, Fos-immunoreactivity was detected in the MePD. Microinjecting the inhibitory neuropeptide somatostatin into the right MePD notably reduces fighting behavior without affecting locomotion. Overall, these data indicate that sex steroids and local neurochemical stimulatory/inhibitory transmitters modulate the MePD and reinforce the idea that this area is a node for modulating social behavior neural networks.

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Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

Section: Morphological Features Of the Medial Amygdalamentioning

confidence: 99%

See 2 more Smart Citations

Morphological and Functional Features of the Sex Steroid-Responsive Posterodorsal Medial Amygdala of Adult Rats

Rasia‐Filho

Haas²,

Oliveira³

et al. 2012

MRMC

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“…Anatomical differences within the amygdala of males and females mainly referred to medial nucleus. They concern volume of nerve nuclei, neuron size, and density of dendritic spines (3,15,19).…”

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confidence: 99%

Immunolocalisation of α and β Oestrogen Receptors in Basolateral Amygdala of Rabbit Males

Lonc

2012

Bulletin of the Veterinary Institute in Pulawy

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Expression of α (ER-α) and β (ER-ß) oestrogen receptors in neurons of basolateral complex of the amygdala was studied in rabbit males. The examinations were carried out on brains of 12 sexually mature rabbit males weighing 3-4 kg. An immunohistochemical method (IHC) with primary Er-α antibodies, NCL-L6F11 and Er-ß: NCL-ERbeta clone EMR02 was applied. The percentage of immunoreactive neurons was determined. Morphometric and statistical analysis of immunoreactive (ER+) neurons was carried out including the localisation of IHC reaction within the examined neurons. The main place of IHC-ER-α reaction was the cytoplasm of nervous cells, whereas in the examination of IHC-ER-β, the reaction was observed mainly within cell nucleus. The nuclei of basolateral complex are a region of relatively high percentage of ER-β+ neurons and lower percentage of ER-α+ neurons in comparison with other nuclei of the amygdala. Basolateral nucleus was characterised by a higher percentage of ER-β+ neurons in which the reaction was observed in the cytoplasm.

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Cellular components of the human medial amygdaloid nucleus

Dall'Oglio

Xavier

Hilbig³

et al. 2012

J of Comparative Neurology

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The medial nucleus (Me) is a superficial component of the amygdaloid complex. Here we assessed the density and morphology of the neurons and glial cells, the glial fibrillary acidic protein (GFAP) immunoreactivity, and the ultrastructure of the synaptic sites in the human Me. The optical fractionator method was applied. The Me presented an estimated mean neuronal density of 1.53 × 10⁵ neurons/mm³ (greater in the left hemisphere), more glia (72% of all cells) than neurons, and a nonneuronal:neuronal ratio of 2.7. Golgi-impregnated neurons had round or ovoid, fusiform, angular, and polygonal cell bodies (10-30 μm in diameter). The length of the dendrites varied, and pleomorphic spines were found in sparsely spiny or densely spiny cells (1.5-5.2 spines/dendritic μm). The axons in the Me neuropil were fine or coarsely beaded, and fibers showed simple or notably complex collateral terminations. The protoplasmic astrocytes were either isolated or formed small clusters and showed GFAP-immunoreactive cell bodies and multiple branches. Furthermore, we identified both asymmetrical (with various small, clear, round, electron-lucent vesicles and, occasionally, large, dense-core vesicles) and symmetrical (with small, flattened vesicles) axodendritic contacts, also including multisynaptic spines. The astrocytes surround and may compose tripartite or tetrapartite synapses, the latter including the extracellular matrix between the pre- and the postsynaptic elements. Interestingly, the terminal axons exhibited a glomerular-like structure with various asymmetrical contacts. These new morphological data on the cellular population and synaptic complexity of the human Me can contribute to our knowledge of its role in health and pathological conditions.

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Dendritic branching features of posterodorsal medial amygdala neurons of adult male and female rats: Further data based on the Golgi method

Cited by 31 publications

References 31 publications

Morphological and Functional Features of the Sex Steroid-Responsive Posterodorsal Medial Amygdala of Adult Rats

Morphological and Functional Features of the Sex Steroid-Responsive Posterodorsal Medial Amygdala of Adult Rats

Immunolocalisation of α and β Oestrogen Receptors in Basolateral Amygdala of Rabbit Males

Cellular components of the human medial amygdaloid nucleus

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