Dendritic cell immune potency on 2D and in 3D collagen matrices

Sapudom, Jiranuwat; Alatoom, Aseel; Mohamed, Walaa Kamal Eddine Ahmad; Garcia-Sabaté, Anna; McBain, Ian; Nasser, Rasha Ayman; Teo, Jeremy C. M.

doi:10.1039/d0bm01141j

Cited by 29 publications

(40 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because DCs are recruited into many abnormal sites or pathological microenvironments such as those associated with inflammation 13 , cancer 14 , or cardiovascular disorders 15 , DC function may be regulated not only by biochemical factors but also by biomechanical stimuli such as substrate stiffness or altered fluid flow in blood and lymphatic organs. For example, the phenotype and function of DCs are altered by the stiffness-controlled two dimensional polyacrylamide gel substrate 16 or collagen based 2D and 3D matrices 17 , suggesting that biomechanical forces may modulate the phenotype and function of DCs under pathological conditions. Moreover, several clinical studies have demonstrated the involvement of DCs in vascular pathology 15 , 18 .…”

Section: Introductionmentioning

confidence: 99%

Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

Kang

Lee

Kim

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. However, little is known about the effects of biomechanical forces on the activation of DCs. Accordingly, here we fabricated a microfluidics system to assess how biomechanical forces affect the migration and activity of DCs during inflammation. Based on the structure of edema, we proposed and experimentally analyzed a novel concept for a microchip model that mimicked such vascular architecture. The intensity of shear stress generated in our engineered chip was found as 0.2–0.6 dyne/cm2 by computational simulation; this value corresponded to inflammation in tissues. In this platform, the directness and FMIs of DCs were significantly increased, whereas the migration velocity of DCs was not altered by shear stress, indicating that mechanical stimuli influenced DC migration. Moreover, DCs with shear stress showed increased expression of the DC activation markers MHC class I and CD86 compared with DCs under static conditions. Taken together, these data suggest that the biomechanical forces are important to regulate the migration and activity of DCs.

show abstract

Section: Introductionmentioning

confidence: 99%

Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

Kang

Lee

Kim

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It was observed, inter alia, that the level of surface markers and secreted proinflammatory cytokines in DCs is higher in 2D cultures compared to the 3D model. Differences in antigen uptake by iDC and mDC-mediated T cell proliferation were also observed in the 3D model (Sapudom et al, 2020).…”

Section: Comparison Of 2d Vs 3d Cell Culture Research Resultsmentioning

confidence: 67%

3D Cell Culture Technology – A New Insight Into in Vitro Research – A Review

Sośniak¹,

Opiela

2021

Annals of Animal Science

View full text Add to dashboard Cite

Most in vitro cell-based research is based on two-dimensional (2D) systems where growth and development take place on a flat surface, which does not reflect the natural environment of the cells. The imperfection and limitations of culture in 2D systems eventually led to the creation of three-dimensional (3D) culture models that more closely reproduce the actual conditions of physiological cell growth. Since the inception of 3D culture technology, many culture models have been developed, such as technologies of multicellular spheroids, organoids, and organs on chips in the technology of scaffolding, hydrogels, bio-printing and liquid media. In this review we will focus on the advantages and disadvantages of the 2D vs. 3D cell cultures technologies. We will also try to sum up available 3D cultures systems and materials for building 3D scaffolds.

show abstract

“…[ 119 ] Sapudom's work emphasized the significance of biophysical cues (i.e., dimensionality, stiffness, and fibrillar density) from the microenvironment in the regulation of DCs immune responses, inviting more research works on DCs mechanoregulation via hydrogel‐like materials. [ 120 ] In addition to DCs, T cell also has mechanosensing ability to sense the mechanical stiffness of their surroundings, which gives us a new avenue to facilitate interactions between T cells and APCs through hydrogel engineering. [ 121 ] For example, combined with anti‐CD3 and anti‐CD28, Teo's group mimicked T cells–DCs interactions in vitro using polyacrylamide gels with defined stiffness according to mature DCs (0.2–25 kPa).…”

Section: Hydrogels To Enhance Cancer Immunotherapymentioning

confidence: 99%

Hydrogels for Engineering the Immune System

Shou

Tay

2021

Advanced NanoBiomed Research

View full text Add to dashboard Cite

Human immune system has evolved as one of the most powerful defense systems to protect against invading pathogens and mutated cells. However, when persistent immune suppression or activation occurs, it can lead to adverse, chronic physiological effects including cancer and arthritis. Hydrogels are soft materials that can be engineered to modulate immune responses through controlled biomolecule release/adsorption, regeneration of lymphoid tissues, and enhanced antigen presentations. This is achieved by programming hydrogels to exhibit optimal properties such as porosity, biodegradability, and biocompatibility to interface seamlessly with the immune system. Herein, recent innovations and future challenges are described using programmable hydrogels to regenerate the lymphatic system, modulate inflammation, and enhance cancer immunotherapy. Key properties of hydrogels are also highlighted for engineering the immune system and techniques to characterize these properties.

show abstract

Dendritic cell immune potency on 2D and in 3D collagen matrices

Abstract: Dendritic cells (DCs) are antigen-presenting cells capable of either activating the immune response or inducing and maintaining immune tolerance. Understanding how biophysical properties affect DC behaviors will provide insight into...

Cited by 29 publications

References 64 publications

Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

3D Cell Culture Technology – A New Insight Into in Vitro Research – A Review

Hydrogels for Engineering the Immune System

Contact Info

Product

Resources

About