Dendritic Cell Vaccines for Cancer Therapy: Fundamentals and Clinical Trials

Romagnoli, Graziela Gorete; Kaneno, Ramon

doi:10.1007/978-3-662-44946-2_19

Cited by 2 publications

(2 citation statements)

References 140 publications

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“…Taken together, the obtained results corroborate our previous observation that exposure of tumor cells to low concentrations of cytotoxic agents increases their immunogenicity (12), reinforcing our hypothesis that changes induced by this treatment can be successfully transferred to DCs by transfection of total tumor RNA, priming them to trigger a specific antitumor response. Besides the number of different protocols proposed to prime DCs, the best way to prepare antitumor therapeutic DC-based vaccine is still unclear (33), leading us to conclude that our protocol deserves a wider investigation in order to be improved for clinical application, especially to reinforce the feasibility of administration of low doses of chemotherapeutic agents in combination with therapeutic DC vaccines. In conclusion these data support and reinforce the idea that simultaneous use of chemotherapy and immunotherapy can be an effective anti-CRC treatment.…”

Section: Discussionmentioning

confidence: 99%

Treatment of colon cancer cells with 5-fluorouracil can improve the effectiveness of RNA-transfected antitumor dendritic cell vaccine

et al. 2017

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Non-cytotoxic concentrations of selected chemotherapeutic agents amplify the antigen presentation capacity of dendritic cells (DCs) and are able to increase the immunogenicity of the colon cancer cell lineage HCT‑116, as previously demonstrated by our group. Since this functional alteration was associated with changes in gene expression, we aimed to evaluate whether transcriptional changes of tumor cells can be transferred to DCs, increasing their ability to induce a specific antitumor response. Therefore, HCT‑116 cells were treated with two different concentrations of 5-fluorouracil (5-FU), and their total RNA was transfected into human monocyte-derived DC, which function was evaluated through their ability to stimulate the proliferation of normal allogeneic T lymphocytes (MLR), and to generate cytolytic T cells. The transfected DCs demonstrated an increased percentage of CD83+, HLA-DR+, CD80+ and CD86+ cells. These phenotypical changes were followed by functional improvement demonstrated by the increased capacity of these DC to induce allogeneic T cell proliferation and to generate specific anti-HCT‑116 cytolytic T cells, as demonstrated by IFN-γ production following in vitro challenge with tumor cells. Our results allow us to conclude that treatment of tumor cells with a non-toxic concentration of 5-FU induces immunogenic changes that are transferred to DC by transfection of total RNA.

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Section: Discussionmentioning

confidence: 99%

Treatment of colon cancer cells with 5-fluorouracil can improve the effectiveness of RNA-transfected antitumor dendritic cell vaccine

et al. 2017

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“…The development of an immune response is strongly dependent on the activity of DCs that are the main APCs and are responsible for triggering a specific antitumor immune response. − Therefore, any material able to change the features and functions of DCs can impact the intensity and quality of the immune response. There are both reports that CNTs can modulate DC activities and that they can carry antigenic materìal for these professional APCs to potentiate an immune response .…”

Section: Impact Of Nanotubes On the Immune Systemmentioning

confidence: 99%

Carbon Nanotube as a Tool for Fighting Cancer

2017

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In 2015, cancer was the cause of almost 22% of deaths worldwide. The high frequency of relapsing diseases and metastasis requires the development of new diagnostic and therapeutic approaches, and the use of nanomaterials is a promising tool for fighting cancer. Among the more extensively studied nanomaterials are carbon nanotubes (CNTs), synthesized as graphene sheets, whose spiral shape is varied in length and thickness. Their physicochemical features, such as the resistance to tension, and thermal and electrical conductivity, allow their application in several fields. In this review, we show evidence supporting the applicability of CNTs in biomedical practice as nanocarriers for drugs and immunomodulatory material, emphasizing their potential for use in cancer treatment.

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Dendritic Cell Vaccines for Cancer Therapy: Fundamentals and Clinical Trials

Cited by 2 publications

References 140 publications

Treatment of colon cancer cells with 5-fluorouracil can improve the effectiveness of RNA-transfected antitumor dendritic cell vaccine

Treatment of colon cancer cells with 5-fluorouracil can improve the effectiveness of RNA-transfected antitumor dendritic cell vaccine

Carbon Nanotube as a Tool for Fighting Cancer

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