Dendritic Cells Are Involved in the Effects of Exercise in a Model of Asthma

MacKenzie, BreAnne; Andrade-Sousa, Adilson Santos; Oliveira-Júnior, Manoel Carneiro; Assumpção-Neto, Erasmo; Brandão-Rangel, Maysa Alves Rodrigues; Silva-Rennó, Adriano; Santos‐Dias, Alana; Cicko, Sanja; Grimm, Melanie; Müller, Tobias; Oliveira, Ana Paula Ligeiro de; Martins, Mílton A.; Idzko, Marco

doi:10.1249/mss.0000000000000927

Cited by 23 publications

(27 citation statements)

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“…While corticosteroid treatment was found to have no effect on mortality outcome in ARDS, whether prophylactic or chronic corticosteroid use affects mortality in the context of sepsis and ARDS is unknown [48]. In concordance with many of our group’s previous studies, in this study, exercise induced IL-10 expression in the BAL as well as parenchymal and epithelial lung cells, and remained elevated for at least 24 h following inoculation [17, 18, 20, 37, 43, 49]. Thus, the anti-inflammatory cytokine IL-10 is not only significantly elevated by exercise alone, levels also persist following a variety of lung injury models, including allergic asthma [17, 18, 20, 37, 49] and lung fibrosis induced by bleomycin [50, 51], COPD [52, 53], and LPS [43, 54].…”

Section: Discussionsupporting

confidence: 80%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 × 10⁴ colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1β (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-α (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-κB expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Pre-incubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-α (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/NF-κB, and redox signaling.

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Section: Discussionsupporting

confidence: 80%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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“…The serial sections were done in approximately 2mm deep into the lung tissue to assure that central to distal airways would be reached in the sections. Picro Sirius Red staining for collagen fibers was performed as previously described [ 9 , 22 – 24 ]. Collagen fibers were detectable via light microscopy (red staining) and the extent of collagen content was determined in the walls of five airways per mouse, in all 8 mice of each group and also in fifteen lung parenchyma fields per mouse (at 20X magnification) using the Image Pro Plus 4.5 software.…”

Section: Methodsmentioning

confidence: 99%

“…Aerobic exercise (AE) has been found to reduce Th2-mediated inflammation in murine allergic asthma models [ 8 , 9 ] and some clinical studies with allergic asthma patients show that exercise may be beneficial [ 10 – 12 ]. In the context of IPF, recent clinical studies have highlighted that while exercise does not cure IPF, pulmonary rehabilitation programs that incorporate physical training improve the patient’s six-minute walk scores, body composition, and quality of life [ 13 – 15 ] with some patients responding more positively than others.…”

Section: Introductionmentioning

confidence: 99%

Aerobic Exercise Attenuated Bleomycin-Induced Lung Fibrosis in Th2-Dominant Mice

et al. 2016

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IntroductionThe aim of this study was to investigate the effect of aerobic exercise (AE) in reducing bleomycin-induced fibrosis in mice of a Th2-dominant immune background (BALB/c).MethodsBALB/c mice were distributed into: sedentary, control (CON), Exercise-only (EX), sedentary, bleomycin-treated (BLEO) and bleomycin-treated+exercised (BLEO+EX); (n = 8/group). Following treadmill adaptation, 15 days following a single, oro-tracheal administration of bleomycin (1.5U/kg), AE was performed 5 days/week, 60min/day for 4 weeks at moderate intensity (60% of maximum velocity reached during a physical test) and assessed for pulmonary inflammation and remodeling, and cytokine levels in bronchoalveolar lavage (BAL).ResultsAt 45 days post injury, compared to BLEO, BLEO+EX demonstrated reduced collagen deposition in the airways (p<0.001) and also in the lung parenchyma (p<0.001). In BAL, a decreased number of total leukocytes (p<0.01), eosinophils (p<0.001), lymphocytes (p<0.01), macrophages (p<0.01), and neutrophils (p<0.01), as well as reduced pro-inflammatory cytokines (CXCL-1; p<0.01), (IL-1β; p<0.001), (IL-5; p<0.01), (IL-6; p<0.001), (IL-13; p<0.01) and pro-fibrotic growth factor IGF-1 (p<0.001) were observed. Anti-inflammatory cytokine IL-10 was increased (p<0.001).ConclusionAE attenuated bleomycin-induced collagen deposition, inflammation and cytokines accumulation in the lungs of mice with a predominately Th2-background suggesting that therapeutic AE (15–44 days post injury) attenuates the pro-inflammatory, Th2 immune response and fibrosis in the bleomycin model.

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“…Além disso, alguns trabalhos demonstraram que animais sensibilizados à ovalbumina apresentaram aumento significativos dos níveis dessas citocinas e havendo redução dos níveis quando o animal é submetido ao protocolo de treinamento, o que corrobora com nossos dados (Silva, et al, 2010;Vieira, et al, 2008;Olivo, et al, 2014;Silva, et al, 2009;Vieira, et al, 2012;Mackenzie et al, 2016). (Walsh, et al, 2011).…”

Section: Discussionunclassified

“…A citocina IL-10 mostrou-se aumentada no grupo sensibilizado a ovalbumina e como esperado apresentou-se mais elevada nos grupos submetidos ao protocolo de treinamento. Alguns estudos já observaram este mesmo fenômeno anti-inflamatório do exercício físico, reduzindo níveis de citocinas IL-4, IL-5 e aumentando os níveis de citocinas IL-10 nos pulmões dos animais submetidos a protocolo de treinamento leve e moderado (Mackenzie et al, 2016;Olivo et al, 2012;Silva, Vieira, Perini, Mauad, & Martins, 2010;Vieira et al, 2012).…”

Section: Discussionunclassified

Imunorregulação induzida pelo exercício físico na inflamação pulmonar alérgica crônica

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Agradeço primeiramente a Deus, por me possibilitar concluir mais uma etapa tão sonhada em minha vida. Agradeço aos meus pais Lourdes e Francisco e meu irmão Felipe, por acreditarem em meus sonhos e me apoiarem em todos os sentidos nas escolhas mais importantes da minha vida. Minha eterna gratidão, por cada momento. Agradeço ao meu melhor amigo e companheiro, Luis, por estar presente nos momentos em que mais precisei durante os últimos anos. Agradeço infinitamente a minha amiga, companheira, "mãezona" e também orientadora, Dra. Fernanda Arantes, por ter me acompanhado todos esses anos, desde a iniciação científica -quando eu não sabia absolutamente nada sobre asma, exercício e todo esse mundo que faz parte da minha vida hoje. Agradeço por toda a paciência, compreensão, generosidade, por ser essa professora maravilhosa que dá os melhores exemplos e faz as melhores analogias. Obrigada por me ensinar o que é ciência e como se faz ciência. Agradeço também pela amizade, confiança e companheirismo. Agradeço a Luana, por ter ajudado nos experimentos e principalmente por ter me inserido no mundo da citometria de fluxo ou melhor dizendo, da imunologia. Agradeço a Dra. Maria Sato, por ter permitido a colaboração com o LIM 56, que possibilitou a realização dos experimentos deste trabalho. Agradeço minha querida amiga Thayse, por ter ajudado nos experimentos e principalmente por ter tirado minhas dúvidas de "Flow Jo", mesmo que seja a distância. Agradeço a grande amiga Bruni, por tirar tantas dúvidas a respeito de imunologia e também por todas as longas conversas a respeito da vida. Agradeço a Rô, por sempre ajudar em tudo o que eu precisei e por ser essa grande amiga que sei que posso contar. Agradeço ao querido amigo Davi, por ter se colocado sempre disponível para me ajudar com os animais e principalmente, pelas orações poderosas que sempre fez por mim. Agradeço a Sara e a Fê Roncon por todos os experimentos que me auxiliaram e também pelas longas conversas. Agradeço a querida amiga "Clarys", por auxiliar em todas as dúvidas a respeito de exercício físico e também por todos os desabafos do dia a dia. Agradeço ao Prof. Dr. Milton e a Dr. Iolanda por terem me permitido fazer parte do laboratório. Aos demais colegas e companheiros do LIM 20, muito obrigada por compartilhar todos esses anos. À USP, pela oportunidade. Muito Obrigada! "A esperança não decepciona." Rm 5,5. NORMALIZAÇÃO ADOTADA Esta dissertação está de acordo com as seguintes normas, em vigor no momento desta publicação: Referências: adaptado de International Committee of Medical Journals Editors (Vancouver).

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Dendritic Cells Are Involved in the Effects of Exercise in a Model of Asthma

Abstract: AE attenuated dendritic cell and lymphocyte activation and maturation, which contributed to reduced airway inflammation and remodeling in the OVA model of chronic allergic airway inflammation.

Cited by 23 publications

References 22 publications

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Aerobic Exercise Attenuated Bleomycin-Induced Lung Fibrosis in Th2-Dominant Mice

Imunorregulação induzida pelo exercício físico na inflamação pulmonar alérgica crônica

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