Dendritic cells in liver transplantation immune response

Du, Xiaodong; Li, Mingqian; Huan, Chen; Lv, Guoyue

doi:10.3389/fcell.2023.1277743

Cited by 3 publications

(1 citation statement)

References 199 publications

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“…Ischemia–reperfusion injury (IRI) is considered a critical factor involved in donor DC maturation and migration to recipient secondary lymphoid tissues. Additionally, Lutz et al proposed that full DC maturation is required for the generation of T-cell responses, whereas Tol-DCs may be in a semi-mature state, inducing Tregs, T-cell weakness, and promoting T-cell apoptosis [ 80 ]. Tol-DC in the liver may be promoted by hepatic stellate cells, which are considered the major source of immunoregulatory molecules, including all trans retinoic acid (ATRA), chemoattractants, such as MCP-1 and MIP-1α, as well as cytokines such as IL-6 and TNF-α.…”

Section: Dendritic Cells and Transplantationmentioning

confidence: 99%

Dendritic Cells: A Bridge between Tolerance Induction and Cancer Development in Transplantation Setting

Troise,

Infante,

Mercuri

et al. 2024

Biomedicines

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Dendritic cells (DCs) are a heterogeneous group of antigen-presenting cells crucial for fostering allograft tolerance while simultaneously supporting host defense against infections and cancer. Within the tumor microenvironment, DCs can either mount an immune response against cancer cells or foster immunotolerance, presenting a dual role. In immunocompromised individuals, posttransplant malignancies pose a significant health concern, with DCs serving as vital players in immune responses against cancer cells. Both recipient- and donor-derived DCs play a critical role in the rejection process, infiltrating the transplanted organ and sustaining T-cell responses. The use of immunosuppressive drugs represents the predominant approach to control this immunological barrier in transplanted organs. Evidence has shed light on the immunopharmacology of these drugs and novel strategies for manipulating DCs to promote allograft survival. Therefore, comprehending the mechanisms underlying this intricate microenvironment and the effects of immunosuppressive therapy on DCs is crucial for developing targeted therapies to reduce graft failure rates. This review will delve into the fundamental immunobiology of DCs and provide a detailed exploration of their clinical significance concerning alloimmune responses and posttransplant malignancies.

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Section: Dendritic Cells and Transplantationmentioning

confidence: 99%

Dendritic Cells: A Bridge between Tolerance Induction and Cancer Development in Transplantation Setting

Troise,

Infante,

Mercuri

et al. 2024

Biomedicines

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A protocol to isolate and characterize pure monocytes and generate monocyte-derived dendritic cells through FBS-Coated flasks

Meskini,

Amanzadeh,

Salehi

et al. 2024

Sci Rep

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This study explores methods to isolate high-pure monocytes and optimize the best growth factor concentration to generate monocytes-derived dendritic cells (mo-DCs), subset DC1, which is crucial in immune responses. Three protocols for monocyte isolation from peripheral blood mononuclear cells (PBMCs) were evaluated: three-hour incubation on FBS-coated flasks; an overnight incubation on FBS-coated flasks; and Magnetic Activated Cell Sorting (MACS). Additionally, five different concentrations of human recombinant granulocyte-macrophage colony-stimulating factor (hrGM-CSF) and human recombinant interleukin-4 (hrIL-4) were compared. We used Flow cytometry to assess the isolation, purification, and generation of pure monocytes characterized as CD14 + , and expression of mo-DC classical markers (HLA-DR, CD80, CD83, and CD86). The obtained results show that monocytes isolated with the second method (overnight incubation) had the highest purity ( P < 0.0001) but the lowest yield ( P > 0.05), balancing purity and cost-effectiveness. A combination of hrGM-CSF and hrIL-4 at 400 U/mL produced the most favorable outcomes, leading to the highest rate of mo-DC generation ( P < 0.05). Notably, this concentration resulted in increasing expression of HLA-DR, CD80, and CD86 surface markers in the generated DCs ( P < 0.0001), with no changes in CD83 expression levels. In conclusion, this study offers valuable insights into selecting the optimal approach for monocyte isolation and mo-DC generation in various research contexts, providing a foundation for more effective immunological studies.

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Mechanisms of Tolerance Induction in Liver Transplantation: Lessons Learned from Fetomaternal Tolerance, Autoimmunity and Tumor Immunity

Nakano,

Goto,

Chen

2024

IJMS

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Since the first published report of experimental kidney transplantation in dogs in 1902, there were many experimental and clinical trials of organ transplantation, with many sacrifices. After the establishment of the surgical technique and the discovery of immunosuppressive drugs, transplantation became the definitive treatment strategy for patients with terminal organ failure. However, this is not a common therapy method due to the difficulty of solving the fundamental issues behind organ transplantation, including the shortage of donor graft, potential risks of transplant surgery and economic capability. The pre- and post-transplant management of recipients is another critical issue that may affect transplant outcome. Most liver transplant recipients experience post-transplant complications, including infection, acute/chronic rejection, metabolic syndrome and the recurrence of hepatocellular carcinoma. Therefore, the early prediction and diagnosis of these complications may improve overall and disease-free survival. Furthermore, how to induce operational tolerance is the key to achieving the ultimate goal of transplantation. In this review, we focus on liver transplantation, which is known to achieve operational tolerance in some circumstances, and the mechanical similarities and differences between liver transplant immunology and fetomaternal tolerance, autoimmunity or tumor immunity are discussed.

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Dendritic cells in liver transplantation immune response

Cited by 3 publications

References 199 publications

Dendritic Cells: A Bridge between Tolerance Induction and Cancer Development in Transplantation Setting

Dendritic Cells: A Bridge between Tolerance Induction and Cancer Development in Transplantation Setting

A protocol to isolate and characterize pure monocytes and generate monocyte-derived dendritic cells through FBS-Coated flasks

Mechanisms of Tolerance Induction in Liver Transplantation: Lessons Learned from Fetomaternal Tolerance, Autoimmunity and Tumor Immunity

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