Dendrobium officinale Enzyme Changing the Structure and Behaviors of Chitosan/γ-poly(glutamic acid) Hydrogel for Potential Skin Care

Wang, Mengmeng; Zhang, Erwei; Yu, Chenrui; Liu, Dandan; Zhao, Shiguang; Xu, Maodong; Zhao, Xiaofeng; Yue, Wenjin; Nie, Guangjun

doi:10.3390/polym14102070

Cited by 10 publications

(4 citation statements)

References 66 publications

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“…However, these limitations can be effectively addressed by modifying the physical and chemical properties and functions of CS/γ-PGA hydrogel. In 2022, Wang et al explored a multi-step non-covalent crosslinking strategy for CS/γ-PGA hydrogel by incorporating Dendrobium candidum enzyme (DOE) ( Figure 4 B) [ 36 ]. They discovered that an increase in γ-PGA content and the addition of DOE resulted in a hydrogel with higher porosity, significantly enhancing its hydrophilicity and water retention.…”

Section: The γ-Pga Hydrogelmentioning

confidence: 99%

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Synthesis of Poly-γ-Glutamic Acid and Its Application in Biomedical Materials

Cai,

Han,

Zheng

et al. 2023

Materials

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Poly-γ-glutamic acid (γ-PGA) is a natural polymer composed of glutamic acid monomer and it has garnered substantial attention in both the fields of material science and biomedicine. Its remarkable cell compatibility, degradability, and other advantageous characteristics have made it a vital component in the medical field. In this comprehensive review, we delve into the production methods, primary application forms, and medical applications of γ-PGA, drawing from numerous prior studies. Among the four production methods for PGA, microbial fermentation currently stands as the most widely employed. This method has seen various optimization strategies, which we summarize here. From drug delivery systems to tissue engineering and wound healing, γ-PGA’s versatility and unique properties have facilitated its successful integration into diverse medical applications, underlining its potential to enhance healthcare outcomes. The objective of this review is to establish a foundational knowledge base for further research in this field.

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Section: The γ-Pga Hydrogelmentioning

confidence: 99%

“… ( A ) The porous structure of hydrogels formed by different PH regulators, different γ-PGA contents, and different drying methods [ 35 ]. ( B ) Effect of DOEs on CS/γ-PGA hydrogel [ 36 ]. ( C ) Preparation principle of HA/γ-PGA [ 41 ].…”

Section: The γ-Pga Hydrogelmentioning

confidence: 99%

Synthesis of Poly-γ-Glutamic Acid and Its Application in Biomedical Materials

Cai,

Han,

Zheng

et al. 2023

Materials

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“…12 Dendrobium nobile polysaccharide plays a crucial role in shielding skin fibroblasts from UVA-induced photoaging 13 and regulating immune cells to mitigate skin inflammation. 14 While recognized for cosmetic benefits, [15][16][17][18][19] Although some studies have explored the potential of Dendrobium ironum for skin repair, there remains a scarcity of specific research regarding its role in promoting wound healing. This knowledge gap highlights the need for further research to unravel the potential of Dendrobium as a wound healing-promoting medicine.…”

Section: Introductionmentioning

confidence: 99%

Anticipation and Verification of Dendrobium-Derived Nanovesicles for Skin Wound Healing Targets, Predicated Upon Immune Infiltration and Senescence

Tu,

Jiang,

Fang

et al. 2024

IJN

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Background: Dendrobium, with profound botanical importance, reveals a rich composition of bioactive compounds, including polysaccharides, flavonoids, alkaloids, and diverse amino acids, holding promise for skin regeneration. However, the precise mechanism remains elusive. Seeking a potent natural remedy for wound healing, exocyst vesicles were successfully isolated from Dendrobium. Aims of the Study: This investigation aimed to employ bioinformatics and in vivo experiments to elucidate target genes of Dendrobium-derived nanovesicles in skin wound healing, focusing on immune infiltration and senescence characteristics. Materials and Methods: C57 mice experienced facilitated wound healing through Dendrobium-derived nanovesicles (DDNVs). Bioinformatics analysis and GEO database mining identified crucial genes by intersecting immune-related, senescence-related, and PANoptosis-associated genes. The identified genes underwent in vivo validation. Results: DDNVs remarkably accelerated skin wound healing in C57 mice. Bioinformatics analysis revealed abnormal expression patterns of immune-related, senescence-related, and pan-apoptosis-related genes, highlighting an overexpressed IL-1β and downregulated IL-18 in the model group, Exploration of signaling pathways included IL-17, NF-kappa B, NOD-like receptor, and Toll-like receptor pathways. In vivo experiments confirmed DDNVs' efficacy in suppressing IL-1β expression, enhancing wound healing. Conclusion: Plant-derived nanovesicles (PDNV) emerged as a natural, reliable, and productive approach to wound healing. DDNVs uptake by mouse skin tissues, labeled with a fluorescent dye, led to enhanced wound healing in C57 mice. Notably, IL-1β overexpression in immune cells and genes played a key role. DDNVs intervention effectively suppressed IL-1β expression, accelerating skin wound tissue repair.

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“…However, most hydrogels possess uneven crosslinking networks constructed with natural macromolecules, without any effective energy dissipation mechanism preventing possible crack propagation or stress concentration. On this account, dendrobium officinale enzyme (DOE) was proposed to improve the structural uniformity and the toughness of chitosan/γ-poly(glutamic acid) (CS/γ-PGA) hydrogel [ 20 ]. The results indicated that DOE with various amounts of ingredients can make multiple noncovalent crosslinks with the skeleton network of CS/γ-PGA, significantly changing the self-assembly of CS/γ-PGA/DOE hydrogel to form regular protuberance nanostructures, exhibiting stronger toughness and better behaviors for skin care.…”

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confidence: 99%