Dendrobium officinale polysaccharide ameliorates diabetic hepatic glucose metabolism via glucagon-mediated signaling pathways and modifying liver-glycogen structure

Liu, Yage; Lin-de, Yang; Zhang, Yu; Liu, Xiaocui; Wu, Zhijing; Gilbert, Robert G.; Deng, Bin; Wang, Kaiping

doi:10.1016/j.jep.2019.112308

Cited by 111 publications

(62 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DOP showed no absorption at 260 nm or 280 nm in the UV spectra, indicating the absence of nucleic acids and proteins in DOP. The structure of DOP was elucidated in our previous study (Liu et al, 2020).…”

Section: Chemical Structure Of Dopmentioning

confidence: 99%

Dendrobium officinale Polysaccharide Protected CCl4-Induced Liver Fibrosis Through Intestinal Homeostasis and the LPS-TLR4-NF-κB Signaling Pathway

Wang

Yang

et al. 2020

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

We explored the therapeutic effects of Dendrobium officinale polysaccharide (DOP) on CCl 4-induced liver fibrosis with respect to the intestinal hepatic axis using a rat model. Histopathological staining results showed that DOP alleviated extensive fibrous tissue proliferation in interstitium and lessened intestinal mucosal damage. Western blot and PCR results showed that DOP maintained intestinal balance by upregulating the expression of tight junction proteins such as occludin, claudin-1, ZO-1, and Bcl-2 proteins while downregulating the expression of Bax and caspase-3 proteins in the intestine. The transepithelial electrical resistance (TEER) value of the LPS-induced Caco-2 monolayer cell model was increased after DOP administration. These illustrated that DOP can protect the intestinal mucosal barrier function. DOP also inhibited activation of the LPS-TLR4-NF-κB signaling pathway to reduce the contents of inflammatory factors TGF-β and TNF-α, increased the expression of anti-inflammatory factor IL-10, and significantly decreased α-SMA and collagen I expression. These results indicated that DOP maintained intestinal homeostasis by enhancing tight junctions between intestinal cells and reducing apoptosis, thereby inhibiting activation of the LPS-TLR4-NF-κB signaling pathway to protect against liver fibrosis.

show abstract

Section: Chemical Structure Of Dopmentioning

confidence: 99%

Dendrobium officinale Polysaccharide Protected CCl4-Induced Liver Fibrosis Through Intestinal Homeostasis and the LPS-TLR4-NF-κB Signaling Pathway

Wang

Yang

et al. 2020

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…catenatum [60]. Studies had reported the first draft genome sequence of D. catenatum and discovered extensive duplication of genes included DcCslD gene family, involved in glucomannan synthase activities, likely related to the synthesis of medicinal polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Cellulose-Like Synthase D Gene Family in Dendrobium Catenatum

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Dendrobium catenatum, which grows on the semi humid rocks in the mountains, has been at the top of the "Nine Immortals of China" since ancient times. It is a kind of yin tonic medicine and its main active component is polysaccharide. Cellulose synthase-like D(CslD) genes were predicted to catalyse the biosynthesis of 1,4-β-d-glycan backbone of hemicelluloses, which plays fundamental roles in plant development. Results: To investigate the role of CslD in the development of Dendrobium catenatum, eight CslD genes (DcCslD1,2a,2b,3a,3b,4a,4b,5) were identified. The results of protein prediction and analysis showed that CslD2a/2b/4a/4b proteins were acidic proteins, the rest were basic proteins; Leu, Ser, Ala, Gly, Arg, Pro and Asp were the main amino acids. All the proteins had obvious hydrophobic or hydrophilic regions, and had transmembrane structure. The main elements of protein secondary structure were α-helix, random coil and extended chain. Phylogenetic analysis revealed that the DcCslD family could be divided into Ⅰ, Ⅱ, Ⅲ and Ⅳ groups. DcCslD proteins had typical Cellulose synthase domain and similar protein structures to the CslDs of other plants. Their promoter regions contain cis regulatory elements related to stress and hormone. The results of qRT-PCR showed that the identified DcCslDs were differentially expressed in roots, stems and leaves. Most of them hightly expressed in stems and leaves. What’s more, the environmental stress1es examination showed that the expressions of DcCslD5 were closely associated with drought-recovery treatment, the expression of DcCslD1, DcCslD2a, DcCslD2b, DcCslD3a, and DcCslD5 were significantly influenced by low temperature. Conclusions: This study systematically analyzed the sequence characteristics of CslD protein of D. catenatum, which can provide reference for further study on the function of CslD protein in polysaccharide metabolism of D. catenatum.

show abstract

“…Thus, another question to ask is whether it is possible to reverse glycogen α particles from fragile state to stable state via lowering blood glucose level? Several studies have attempted to repair glycogen α-particle fragility by using well-known anti-diabetic drugs ( Li et al, 2019 ; Liu et al, 2020c , d ). In specificity, Li et al (2019) first checked the influences of active components from four anti-diabetic traditional Chinese medicines (TCMs) on the repairment of glycogen structural fragility, which includes astragalus polysaccharide (APS), berberine (BBR), panax notoginseng saponins (PNS) and pueraria flavonoid (PF).…”

Section: Potential Drug Target For Diabetic Therapymentioning

confidence: 99%

From Prokaryotes to Eukaryotes: Insights Into the Molecular Structure of Glycogen Particles

Liu

Tang

Wen

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

Glycogen is a highly-branched polysaccharide that is widely distributed across the three life domains. It has versatile functions in physiological activities such as energy reserve, osmotic regulation, blood glucose homeostasis, and pH maintenance. Recent research also confirms that glycogen plays important roles in longevity and cognition. Intrinsically, glycogen function is determined by its structure that has been intensively studied for many years. The recent association of glycogen α-particle fragility with diabetic conditions further strengthens the importance of glycogen structure in its function. By using improved glycogen extraction procedures and a series of advanced analytical techniques, the fine molecular structure of glycogen particles in human beings and several model organisms such as Escherichia coli, Caenorhabditis elegans, Mus musculus, and Rat rattus have been characterized. However, there are still many unknowns about the assembly mechanisms of glycogen particles, the dynamic changes of glycogen structures, and the composition of glycogen associated proteins (glycogen proteome). In this review, we explored the recent progresses in glycogen studies with a focus on the structure of glycogen particles, which may not only provide insights into glycogen functions, but also facilitate the discovery of novel drug targets for the treatment of diabetes mellitus.

show abstract

Dendrobium officinale polysaccharide ameliorates diabetic hepatic glucose metabolism via glucagon-mediated signaling pathways and modifying liver-glycogen structure

Cited by 111 publications

References 37 publications

Dendrobium officinale Polysaccharide Protected CCl4-Induced Liver Fibrosis Through Intestinal Homeostasis and the LPS-TLR4-NF-κB Signaling Pathway

Dendrobium officinale Polysaccharide Protected CCl4-Induced Liver Fibrosis Through Intestinal Homeostasis and the LPS-TLR4-NF-κB Signaling Pathway

Genome-Wide Identification of Cellulose-Like Synthase D Gene Family in Dendrobium Catenatum

From Prokaryotes to Eukaryotes: Insights Into the Molecular Structure of Glycogen Particles

Contact Info

Product

Resources

About