Dendrosomal Curcumin Inhibits Metastatic Potential of Human SW480 Colon Cancer Cells through Down-regulation of Claudin1, Zeb1 and Hef1-1 Gene Expression

Abstract

Colon cancer is one of the leading causes of cancer-associated death worldwide. The prognosis for advanced colorectal cancers remains dismal, mainly due to the propensity for metastatic progression. Accordingly, there is a need for effective anti-metastasis therapeutic agents. Since a great body of research has indicated anticancer effects for curcumin, we investigated the effects of dendrosomal curcumin (DNC) on cellular migration and adhesion of human SW480 cells and possible molecular mechanisms involved. Different methods were applied in this study including MTT, Scratch and adhesion assays as well as real-time PCR and transwell chamber assays. Based on the results obtained, DNC inhibits metastasis by decreasing Hef 1, Zeb 1 and Claudin 1 mRNA levels and can reduce SW480 cell proliferation with IC 50 values of 15.9, 11.6 and 7.64 µM at 24, 48 and 72h posttreatment. Thus it might be considered as a safe formulation for therapeutic purpose in colorectal cancer cases.

Anti-proliferative and Apoptotic Effects of Dendrosomal Farnesiferol C on Gastric Cancer Cells

“…We have demonstrated that by employing our dendrosome nano-particles in vitro, the solubility of FC thereby its anti-proliferation effect on cancer cells could be improved. Dendrosomes are neutral, covalent, selfassembled, biodegradable and spherical nano-particles and their exceptional capabilities have been shown in safely delivering genes and herbal compounds into the different cell lines (Sarbolouki et al, 2000;Sadeghizadeh et al, 2008;Mashinchian et al, 2010;Babaei et al, 2012, Dehghan Esmatabadi et al, 2015. Current work covers the study of anti-proliferation and apoptotic effects of DFC compared to FC.…”

Current status and future prospective of Curcumin as a potential therapeutic agent in the treatment of colorectal cancer

“…A lipophilic analog of curcumin, Dimethoxy curcumin, inhibits the growth of CRC by downregulating survivin and upregulating E‐cadherin of CRC cells (Chen et al, ). Dendrosomal curcumin prevents metastatic potential of CRC cells via downregulation of cell adhesion proteins including Hef‐1, Zeb‐1, and Claudin‐1 (Dehghan Esmatabadi et al, ). Similarly, Su et al indicated that curcumin suppresses invasion and metastasis of CRC via modulation of NF‐κB and downregulation of COX‐2 and matrix metalloproteinase‐2 (MMP‐2) expressions (Su, Chen, Lin, Wu, & Chung, ).…”

Expression Pattern and Prognostic Significance of Claudin 1, 4 and 7 in Pancreatic Cancer

“…In a study of Dehghan Esmatabadi, et al (2015), the effects of dendrosomal curcumin (DNC) on cellular migration and adhesion of human SW480 colon cancer cells were searched and DNC was found to inhibit metastasis by causing a decrease in mRNA levels of some proteins including Claudin 1, Hef 1 and Zeb 1 (Dehghan Esmatabadi et al, 2015). Tan et al (2004) showed that expression and dispersion of Claudin 1 is related with dissociation of the pancreatic cancer cells (Tan et al, 2004).…”