Background: Dengue, the mosquito borne disease has become a growing public health threat in Bangladesh due to its gradual increasing morbidity and mortality since 2000. In 2019, the country witnessed the worst ever dengue outbreak. The present study was conducted to characterize the socio-economic factors and knowledge, attitude and practice (KAP) status towards dengue among the people of Bangladesh.Method: A cross-sectional study was conducted with 1,010 randomly selected participants from nine different administrative regions of Bangladesh between July and November 2019. A well-structured questionnaire was used covering socio-demographic characteristics of the participants including their knowledge, awareness, treatment and practices regarding Dengue fever.Results: Although majority (93.8%) of the participants had heard about dengue, however, they had still misconceptions about Aedes breeding habitat. Around half of the study population (45.7%) had mistaken belief that Aedes can breed in dirty water and 43.1% knew that Aedes mosquito usually bites during sunrise/sunset. Fever indication was found in 36.6% of people which is the most common symptom of dengue. Living place, literacy and employment status were found significantly associated (p<0.05) with knowledge and awareness of dengue fever. The preventive practice level was moderately less than the knowledge level though there was a significant association (p<0.05) existed between knowledge and preventive practices. Our study noted that TV/Radio is an effective predominant source of information about dengue fever.Conclusion: As dengue is emerging in Bangladesh, there is a need to increase health promotion activities through campaigns for eliminating the misconception and considerable knowledge gaps about dengue.