Dengue Infection Increases the Locomotor Activity of Aedes aegypti Females

Lima-Camara, Tamara Nunes; Bruno, Rafaela Vieira; Luz, Paula M.; Castro, Márcia Gonçalves de; Lourenço‐de‐Oliveira, Ricardo; Sorgine, Marcos Henrique Ferreira; Peixoto, Alexandre A.

doi:10.1371/journal.pone.0017690

Cited by 131 publications

(122 citation statements)

References 34 publications

Supporting

Mentioning

109

Contrasting

Unclassified

Order By: Relevance

“…Our results complement and extend previous findings regarding plant pathogen-induced behavioral manipulations of vectors, which focused mainly on indirect changes of host choice behavior and feeding mediated by changes in host (plant) chemistry [13, 39, 40]. Our observation of pathogen-induced increases in flight initiation closely parallel findings of pathogen infection modifying insect host locomotor activity to facilitate their spread, such as the pathogen-induced host climbing behavior of Lepidoptera larvae and increased activity of Aedes aegypti [41, 42]. …”

Section: Discussionsupporting

confidence: 78%

Infection of an Insect Vector with a Bacterial Plant Pathogen Increases Its Propensity for Dispersal

et al. 2015

View full text Add to dashboard Cite

The spread of vector-transmitted pathogens relies on complex interactions between host, vector and pathogen. In sessile plant pathosystems, the spread of a pathogen highly depends on the movement and mobility of the vector. However, questions remain as to whether and how pathogen-induced vector manipulations may affect the spread of a plant pathogen. Here we report for the first time that infection with a bacterial plant pathogen increases the probability of vector dispersal, and that such movement of vectors is likely manipulated by a bacterial plant pathogen. We investigated how Candidatus Liberibacter asiaticus (CLas) affects dispersal behavior, flight capacity, and the sexual attraction of its vector, the Asian citrus psyllid (Diaphorina citri Kuwayama). CLas is the putative causal agent of huanglongbing (HLB), which is a disease that threatens the viability of commercial citrus production worldwide. When D. citri developed on CLas-infected plants, short distance dispersal of male D. citri was greater compared to counterparts reared on uninfected plants. Flight by CLas-infected D. citri was initiated earlier and long flight events were more common than by uninfected psyllids, as measured by a flight mill apparatus. Additionally, CLas titers were higher among psyllids that performed long flights than psyllid that performed short flights. Finally, attractiveness of female D. citri that developed on infected plants to male conspecifics increased proportionally with increasing CLas bacterial titers measured within female psyllids. Our study indicates that the phytopathogen, CLas, may manipulate movement and mate selection behavior of their vectors, which is a possible evolved mechanism to promote their own spread. These results have global implications for both current HLB models of disease spread and control strategies.

show abstract

Section: Discussionsupporting

confidence: 78%

Infection of an Insect Vector with a Bacterial Plant Pathogen Increases Its Propensity for Dispersal

et al. 2015

View full text Add to dashboard Cite

show abstract

“…For this reason, investigating host activity throughout infection is useful to interpret changes in MR and energy budgeting. Mosquitoes infected with dengue virus were more active than uninfected individuals (Lima-Camara et al, 2011). However, dengue virus demonstrates tropism for nervous tissues, so changes in activity would be expected (Salazar et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Physiological and metabolic consequences of viral infection in Drosophila melanogaster

Arnold

Johnson

White

2013

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYAn extensively used model system for investigating anti-pathogen defence and innate immunity involves Drosophila C virus (DCV) and Drosophila melanogaster. While there has been a significant effort to understand infection consequences at molecular and genetic levels, an understanding of fundamental higher-level physiology of this system is lacking. Here, we investigate the metabolic rate, locomotory activity, dry mass and water content of adult male flies injected with DCV, measured over the 4days prior to virus-induced mortality. DCV infection resulted in multiple pathologies, notably the depression of metabolic rate beginning 2days post-infection as a response to physiological stress. Even in this depressed metabolic state, infected flies did not decrease their activity until 1day prior to mortality, which further suggests that cellular processes and synthesis are disrupted because of viral infection. Growth rate was also reduced, indicating that energy partitioning is altered as infection progresses. Microbial infection in insects typically results in an increase in excretion; however, water appeared to be retained in DCV-infected flies. We hypothesise that this is due to a fluid intake-output imbalance due to disrupted transport signalling and a reduced rate of metabolic processing. Furthermore, infected flies had a reduced rate of respiration as a consequence of metabolic depression, which minimised water loss, and the excess mass as a result of water retention is concurrent with impaired locomotory ability. These findings contribute to developing a mechanistic understanding of how pathologies accumulate and lead to mortality in infected flies.

show abstract

“…A few reports suggest a possible alteration in the general behavior of infected arthropods. Some reports show that insects can present significant modifications in their way to fly and feed following infection by dengue virus (DV), an important human pathogenic arbovirus of the Flaviviridae family [48,49]. Regarding bunyaviruses, infection of Aedes mosquitoes by LACV seems to cause a higher biting activity and also a longer probing time [50,51].…”

Section: Virus Transmissionmentioning

confidence: 99%

Bunyaviruses: From Transmission by Arthropods to Virus Entry into the Mammalian Host First-Target Cells

Léger

Lozach

2015

Future Virol.

View full text Add to dashboard Cite

The Bunyaviridae constitute a large family of animal RNA viruses distributed worldwide, most members of which are transmitted to vertebrate hosts by arthropods and can cause severe pathologies in humans and livestock. With an increasing number of outbreaks, arthropod-borne bunyaviruses (arbo-bunyaviruses) present a global threat to public health and agricultural productivity. Yet transmission, tropism, receptors and cell entry remain poorly characterized. The focus of this review is on the initial infection of mammalian hosts by arbo-bunyaviruses from cellular and molecular perspectives, with particular attention to the human host. We address current knowledge and advances regarding the identity of the first-target cells and the subsequent processes of entry and penetration into the cytosol. Aspects of the vector-to-host switch that influence the early steps of cell infection in mammalian skin, where incoming particles are introduced by infected arthropods, are also highlighted and discussed.

show abstract

Dengue Infection Increases the Locomotor Activity of Aedes aegypti Females

Cited by 131 publications

References 34 publications

Infection of an Insect Vector with a Bacterial Plant Pathogen Increases Its Propensity for Dispersal

Infection of an Insect Vector with a Bacterial Plant Pathogen Increases Its Propensity for Dispersal

Physiological and metabolic consequences of viral infection in Drosophila melanogaster

Bunyaviruses: From Transmission by Arthropods to Virus Entry into the Mammalian Host First-Target Cells

Contact Info

Product

Resources

About