2013
DOI: 10.1186/1423-0127-20-65
|View full text |Cite
|
Sign up to set email alerts
|

Dengue virus infection induces autophagy: an in vivo study

Abstract: BackgroundWe and others have reported that autophagy is induced by dengue viruses (DVs) in various cell lines, and that it plays a supportive role in DV replication. This study intended to clarify whether DV infection could induce autophagy in vivo. Furthermore, the effect of DV induced autophagy on viral replication and DV-related pathogenesis was investigated.Results and conclusionsThe physiopathological parameters were evaluated after DV2 was intracranially injected into 6-day-old ICR suckling mice. Autopha… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

7
67
0
2

Year Published

2014
2014
2021
2021

Publication Types

Select...
7
2

Relationship

0
9

Authors

Journals

citations
Cited by 69 publications
(76 citation statements)
references
References 49 publications
7
67
0
2
Order By: Relevance
“…Here, we report findings that suggest a biphasic response of autophagy to DENV infection, where DENV infection initially activates and then later on inhibits autophagy. Although it has been reported by several groups that multiple components of the autophagy pathway are required for DENV replication (4,6,7,(27)(28)(29), we demonstrate here that DENV activates autophagy only during the early infection stage. Moreover, we established that, by 24 h after infection with the NGC strain, autophagosome formation is inhibited and lysosomal degradation of AVs is reduced.…”
Section: Discussionmentioning
confidence: 70%
See 1 more Smart Citation
“…Here, we report findings that suggest a biphasic response of autophagy to DENV infection, where DENV infection initially activates and then later on inhibits autophagy. Although it has been reported by several groups that multiple components of the autophagy pathway are required for DENV replication (4,6,7,(27)(28)(29), we demonstrate here that DENV activates autophagy only during the early infection stage. Moreover, we established that, by 24 h after infection with the NGC strain, autophagosome formation is inhibited and lysosomal degradation of AVs is reduced.…”
Section: Discussionmentioning
confidence: 70%
“…Autophagic flux is determined by the capacity of the cell to degrade forming autophagosomes, which occurs within minutes (25), and AV content increases upon disruption to lysosomal activity. It is commonly reported that DENV infection increases AV number as detected by high-resolution microscopy (6,7,24,26,27) and Western blot analysis of lipidated LC3-II levels (5,6,28,29). Notably, in autophagosomes colocalizing with lysosomes, pronounced pH-dependent quenching of green fluorescent protein (GFP)-LC3 fluorescence (30) was not observed (7), suggesting that DENV may reduce lysosomal acidification and so disrupt lysosomal function.…”
mentioning
confidence: 99%
“…The protein is itself degraded by autophagy. 12 As shown in Figure 4 and Supplementary Figure S2, treatment of Salmonella in melanoma cells dramatically decreased the expression of p62. These results suggested that Salmonella induced melanoma autophagy.…”
Section: Resultsmentioning
confidence: 91%
“…However, this hypothesis is not consistent with data obtained by cryo-electron tomography, which revealed that viral RNA replication would take place in endoplasmic reticulum invaginations [99]. On the other hand, Heaton et al found that induction of autophagy in DENV-infected cells would be implied in the alteration of lipid metabolism and a correlation between autophagy-dependent degradation of lipid droplets, which are cellular stores of triglycerides and cholesterol esters, and viral replication has been assessed [97,99,100]. Furthermore, it has been recently shown that antibody-enhanced DENV infection also induces autophagy in pre-basophil-like KU812 cells and immature mast cell-like HMC-1 cells [101].…”
Section: • • Intracellular Trafficking and Viral Uncoatingmentioning
confidence: 80%