Dengue Virus Selectively Induces Human Mast Cell Chemokine Production

King, Christine A.; Anderson, Robert; Marshall, Jean S.

doi:10.1128/jvi.76.16.8408-8419.2002

Cited by 149 publications

(146 citation statements)

References 73 publications

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“…These results were unexpected since mast cells infected with DENV in vitro produce increased levels of several chemokines including CCL5 (RANTES) and CXCL10 (IP-10) 13,16 . The present in vivo study showing …”

Section: Discussionmentioning

confidence: 61%

“…Previous in vitro studies indicated that antibody-enhanced DENV infection of mast cells induces chemokines CCL3 (MIP-1a), CCL4 (MIP-1b) and CCL5 (RANTES) 13 as well as cytokines IL-6, IL-1b and TNF-a production.…”

Section: Discussionmentioning

confidence: 99%

“…11 Mast cellderived chymase also promotes vascular leakage in DENV-infected mice. 12 In vitro studies indicated that antibody-enhanced DENV infection of mast cells selectively induces production of chemokines CCL3 (MIP-1a), CCL4 (MIP-1b) and CCL5 (RANTES), 13 as well as cytokines IL-6, IL-1b and TNF-a. 14 The TNF-a produced from antibody-enhanced DENV infection of mast cells can trigger endothelial cell activation.…”

Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

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Mast cell–macrophage dynamics in modulation of dengue virus infection in skin

et al. 2015

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SummaryDengue virus (DENV) infection causes dengue fever, dengue haemorrhagic fever, or dengue shock syndrome. Mast cells have been speculated to play a role in DENV disease although their precise roles are unclear. In this study, we used mast cell-deficient Kit W-sh/W-sh mice to investigate the involvement of mast cells after intradermal DENV infection. An approximately two-to three-fold higher level of DENV NS3 antigen was detected at the skin inoculation site in DENV-infected Kit W-sh/W-sh mice than in DENV-infected wild-type (WT) mice (using a dose of 1 3 10 9 plaqueforming units/mouse). Moreover, as an indicator of heightened pathogenesis, a more prolonged bleeding time was observed in DENV-infected Kit W-sh/W-sh mice than in WT mice. Monocytes/macrophages are considered to be important targets for DENV infection, so we investigated the susceptibility and chemokine response of DENV-infected peritoneal macrophages from Kit W-sh/W-sh and WT mice both ex vivo and in vivo. There was a tendency for higher DENV infection and higher secretion of CCL2 (MCP-1) from peritoneal macrophages isolated from Kit W-sh/W-sh mice than those from WT mice. In vivo studies using intradermal inoculation of DENV showed about twofold higher levels of infiltrating macrophages and CCL2 (MCP-1) at the inoculation site in both mock control and DENV-inoculated Kit W-sh/W-sh mice than in corresponding WT mice. In summary, compared with WT mice, Kit W-sh/W-sh mice show enhanced DENV infection and macrophage infiltration at the skin inoculation site as well as increased DENV-associated bleeding time. The results indicate an intriguing interplay between mast cells and tissue macrophages to restrict DENV replication in the skin.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

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Mast cell–macrophage dynamics in modulation of dengue virus infection in skin

et al. 2015

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“…6 It is likely that viral infection stimulates a variety of intracellular signaling pathways in human MCs. Previous reports have shown that dengue virus infection of human MCs stimulates RANTES, macrophage inflammatory protein 1a, and macrophage inflammatory protein 1b, 24 which may recruit inflammatory cells to sites of infection. Still others have shown that rhinovirus infection alone does not alter cytokine production by human basophilic or MC lines 25 but can potentiate IgE-activated IL-4, IL-6, and IL-8 release.…”

Section: Discussionmentioning

confidence: 99%

Activation of mast cells by double-stranded RNA: evidence for activation through Toll-like receptor 3

Kulka

Alexopoulou

Flavell

et al. 2004

Journal of Allergy and Clinical Immunology

313

318

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“…In studies with cases of DHF and DSS, increase of levels of histamine in urine was observed (Tuchinda et al 1977). Studies with human mast cell culture infected with dengue virus suggest a role for these cells in the initiation of chemokinedependent host responses to dengue virus infection (King et al 2002). Studies with mice inoculated with a cytotoxic factor obtained of spleen of mice infected with DENV showed alterations of the blood-brain barrier, leading to oedema, which was mediated via liberation of histamine (Chaturvedi 1991).…”

Section: Discussionmentioning

confidence: 99%

Histopathological and ultrastructural aspects of mice lungs experimentally infected with dengue virus serotype 2

Barreto¹,

Takiya²,

Schatzmayr³

et al. 2007

Mem. Inst. Oswaldo Cruz

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Key words: dengue-2 virus -BALB/c mice -C6/36 cell line -macrophage -mast cell -ultrastructure Dengue fever (DF) is an acute infectious disease caused by dengue virus (DENV) (Halstead 1988, Henchal & Putnak 1990) that belong to the Flavivirus genus of the Flaviviridae family of single-stranded, positive-polarity, enveloped RNAviruses (Chambers et al. 1990). Dengue disease (DEN) has a spectrum of clinical signs and symptoms, ranging from asymptomatic infection to severe and lethal manifestations. The four DENV serotypes (DENV-1, -2, -3, -4) may cause dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) in humans, with an estimated 100 million new cases of DF and over 250,000 cases of DHF/DSS per year worldwide. The major difficulty in studying DENV infection in humans and for developing a vaccine, is the absence of a suitable animal model which develops a disease with similar aspects of the DHF and DSS (Bhamarapravati 1993). Several studies suggest that mice are a permissive host for DENV (Meiklejhon et al. 1952, Lin et al.1998, Johnson & Roehrig 1999), but in the majority of these models the animals are im- (Table). In the present study we characterized the injuries caused by DENV-2 infection in lung tissue of BALB/c mice using photonic and electron transmission microscopies. Differing from the majority of the mice models, the DENV-2 (nonneuroadapted) were obtained from a patient serum, propagated once in the C6/36 cell line and inoculated by intravenous (i.v.) and intraperitoneal (i.p.) routes. MATERIALS AND METHODS Virus -The virus strain used in our experiments was isolated from a patient serum during an epidemic of DENV-2 in the state of Rio de Janeiro in 1995 and propagated in the Aedes albopictus mosquito cell line (C6/ 36). The serum was tested by the indirect immunofluorescence technique using a type specific DENV-2 monoclonal (3H5, DENV-2) antibody. The virus had not undergone passage in mouse brain. The titer of the virus (10 6.3 TCID 50 /0.1 ml) was calculated by the method of Reed and Muench (1938).Cells -The C6/36 monolayers were grown in L-15 medium supplemented with 1% non-essencial aminoacids, 10% tryptose phosphate broth, and 10% fetal bovine serum. The tubes were kept at 28 o C.Animal -Adult male BALB/c mice, aged 2 months and weighing 25 g, were obtained from the mouse colony maintained in the Department of Virology of the Instituto Oswaldo Cruz-Fiocruz. Mice were i.p. and i.v. inoculated with DENV-2 (dose of 10,000 TCID 50 /0.2 ml) and sacrificed after 48, 72 h, 7 and 49 days p.i. Non-infected mice and mice inoculated with L-15 medium were used as controls and sacrificed at the same time. The experiments were previously approved by the Animal Experimentation Ethical Committee of the Instituto Oswaldo Cruz-Fiocruz.Processing of tissues for photonic microscopy analysis -The animals were peritoneally anaesthetized and lung tissue fragments were collected from infected and non-infected mice. Samples were fixed in Millonig's fixative, dehydrated in ethanol, and paraffin-embedded. Sections (5...

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Dengue Virus Selectively Induces Human Mast Cell Chemokine Production

Cited by 149 publications

References 73 publications

Mast cell–macrophage dynamics in modulation of dengue virus infection in skin

Mast cell–macrophage dynamics in modulation of dengue virus infection in skin

Activation of mast cells by double-stranded RNA: evidence for activation through Toll-like receptor 3

Histopathological and ultrastructural aspects of mice lungs experimentally infected with dengue virus serotype 2

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