Dengue Viruses

Schlesinger, R. Walter

doi:10.1007/978-3-7091-8466-0

Cited by 46 publications

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“…The virus was resuspended in either TNE buffer (10 mM Tris-HCl pH 7n5 ; 100 mM NaCl ; 1 mM EDTA) or RPMI and stored in 1 ml aliquots at k70 mC. Since dengue-3 virus strain 1327 is non-cytopathic, all viruses were quantified by haemagglutination assay, where the number of virus particles equals the number of red blood cells in the reaction mixture (Schlesinger, 1977).…”

Section: Methodsmentioning

confidence: 99%

Analysis of a recombinant dengue-2 virus-dengue-3 virus hybrid envelope protein expressed in a secretory baculovirus system.

Bielefeldt‐Ohmann

Beasley

FitzPatrick

et al. 1997

Journal of General Virology

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In a step towards a tetravalent dengue virus subunit vaccine which is economical to produce, highly immunogenic and stable, a hybrid dengue virus envelope (E) protein molecule has been constructed. It consists of 36 amino acids from the membrane protein, the N-terminal 288 amino acids of the dengue-2 virus E protein plus amino acids 289-424 of the dengue-3 virus E protein. It has been engineered for secretory expression by fusion to a mellitin secretory signal sequence and truncation of the hydrophobic transmembrane segment. Using the baculovirus expression system and serumfree conditions, more than 95 % of recombinant dengue-2 virus-dengue-3 virus hybrid E protein (rD2D3E) was secreted into the cell culture supernatant in a stable form with multiple features indicative of preserved conformation. The hybrid molecule reacted with a panel of dengue virus-and

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Section: Methodsmentioning

confidence: 99%

Analysis of a recombinant dengue-2 virus-dengue-3 virus hybrid envelope protein expressed in a secretory baculovirus system.

Bielefeldt‐Ohmann

Beasley

FitzPatrick

et al. 1997

Journal of General Virology

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“…Four distinct serotypes of dengue virus can be differentiated and all have been implicated in both classical dengue fever and cases with haemorrhagic complications (Schlesinger, 1977).…”

Section: Introductionmentioning

confidence: 99%

“…Although classical dengue fever is a mild illness of man dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS), more serious diseases first recorded in the Philippines in 1954, are major causes of paediatric morbidity and mortality in 12 south-east Asian countries (Schlesinger, 1977). Dengue viruses are endemic in many parts of the world but, until recently, the problem of DHF/DSS was restricted to south-east Asia.…”

Section: Introductionmentioning

confidence: 99%

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Walker

Henchal

Blok

et al. 1988

Journal of General Virology

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SUMMARYDengue type-2 viruses isolated in metropolitan Bangkok during 1980 (Bangkok/80) were characterized by oligonucleotide fingerprinting, restriction enzyme (RE) mapping and antigenic analysis using monoclonal antibody probes. Of 10 isolates analysed by oligonucleotide fingerprinting, nine were very closely related, showing 72-5 ~o to 91.4 ~ oligonucleotide homology. One isolate (D80-141) produced a distinctly different fingerprint (55.7~ to 58"0~o homology) and was less related to other Bangkok/80 dengue-2 virus isolates than to a 1964 Bangkok isolate (16681). RE mapping conducted on complementary dsDNA prepared from three Bangkok/80 isolates, strain 16681 and the prototype New Guinea C strain confirmed that D80-141 was genetically distinct. On antigenic analysis, only one of 22 monoclonal antibody probes produced against representative 1980 Bangkok dengue-2 isolates, D80-100 and D80-141, was able to distinguish between these virus strains. Monoclonal antibody 47-10/10, prepared using D80-100 virus and directed at the NS1 non-structural glycoprotein, had a significantly lower (100-fold) solid phase radioimmune assay endpoint titre for D80-141 antigen than for D80-100 antigen. By the indirect immunofluorescence assay, 47-10/10 had lower antibody endpoint titres against D80-141, the NGC strain and 13 (12~o) of 110 Bangkok/80 isolates than to a control antibody preparation. These results suggest that strain D80-141 represents a second minor topotype of dengue-2 which was circulating concurrently with the major endemic topotype in Bangkok in early 1980.

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“…The outcome of infection with any one of the four serotypes of dengue virus depends critically upon the immune status ofthe individual at the time of exposure. Infection in a non-immune subject results only in a benign, self-limiting febrile illness, usually associated with a rash; this is classical dengue fever, which may involve painful joint symptoms (break-bone fever), but is virtually never fatal (Siler, Hall & Hitchens, 1926;Schlesinger, 1977). Infection in an individual with antibodies depends upon the specificity of the relationship between those antibodies and the infecting dengue virus.…”

mentioning

confidence: 99%

Immunological enhancement and the pathogenesis of dengue haemorrhagic fever

Porterfield

1982

J. Hyg.

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SummaryLaboratory studies have provided evidence that the replication of dengue viruses in preparations of primary peripheral blood mononuclear cells of human or simian origin, or in macrophage-like cell lines of human or murine origin, may be enhanced by sub-neutralizing concentrations of homotypic dengue antibody, by heterotypic dengue antibody, or by antibody against heterologous flaviviruses. The mechanism underlying this phenomenon is discussed in the context of dengue haemorrhagic fever and the dengue shock syndrome.

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Dengue Viruses

Cited by 46 publications

References 0 publications

Analysis of a recombinant dengue-2 virus-dengue-3 virus hybrid envelope protein expressed in a secretory baculovirus system.

Analysis of a recombinant dengue-2 virus-dengue-3 virus hybrid envelope protein expressed in a secretory baculovirus system.

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Immunological enhancement and the pathogenesis of dengue haemorrhagic fever

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