Denitrification and nitrate-reducing bacterial populations in abandoned and reclaimed minesoils

Shirey, J J; Sexstone, Alan J.

doi:10.1111/j.1574-6968.1989.tb03658.x

Cited by 22 publications

(3 citation statements)

References 37 publications

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“…Based on the congruence between the 16S rDNA and narG phylogenies (Philippot, 2002), our present results revealed that the narG sequences obtained are affiliated to highly diverse bacterial groups such as the Actinomycetales, the γ or the β Proteobacteria. The taxonomic divisions observed in this study concurred with the finding of previous studies based either on cultivation‐dependent or cultivation‐independent techniques (Nijburg et al ., 1997a,b; Shirey and Sextone, 1989). However, most of the narG sequences discovered in this study belong to cluster 4, 5 and 6 and were not affiliated with any narG sequence from known bacteria.…”

Section: Discussionmentioning

confidence: 99%

Influence of maize mucilage on the diversity and activity of the denitrifying community

Mounier

Hallet

Chèneby

et al. 2004

Environmental Microbiology

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In order to understand the effect of the maize rhizosphere on denitrification, the diversity and the activity of the denitrifying community were studied in soil amended with maize mucilage. Diversity of the denitrifying community was investigated by polymerase chain reaction (PCR) amplification of total community DNA extracted from soils using gene fragments, encoding the nitrate reductase (narG) and the nitrous oxide reductase (nosZ), as molecular markers. To assess the underlying diversity, PCR products were cloned and 10 gene libraries were obtained for each targeted gene. Libraries containing 738 and 713 narG and nosZ clones, respectively, were screened by restriction fragment analysis, and grouped based on their RFLP (restriction fragment length polymorphism) patterns. In all, 117 and 171 different clone families have been identified for narG and nosZ and representatives of RFLP families containing at least two clones were sequenced. Rarefaction curves of both genes did not reach a clear saturation, indicating that analysis of an increasing number of clones would have revealed further diversity. Recovered NarG sequences were related to NarG from Actinomycetales and from Proteobacteria but most of them are not related to NarG from known bacteria. In contrast, most of the NosZ sequences were related to NosZ from alpha, beta, and gammaProteobacteria. Denitrifying activity was monitored by incubating the control and amended soils anaerobically in presence of acetylene. The N2O production rates revealed denitrifying activity to be greater in amended soil than in control soil. Altogether, our results revealed that mucilage addition to the soil results in a strong impact on the activity of the denitrifying community and minor changes on its diversity.

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Section: Discussionmentioning

confidence: 99%

Influence of maize mucilage on the diversity and activity of the denitrifying community

Mounier

Hallet

Chèneby

et al. 2004

Environmental Microbiology

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“…There are reports that the soil microbial communities which reduce nitrate differ as a function of temperature (202,278,449) and pH (41,392,490). There are also numerous reports that the production and release of NO and N 2 O from soil into the atmosphere are affected by soil temperature and pH (213,434,451,578).…”

Section: Composition Of the Microbial Community And Control Of Trace mentioning

confidence: 99%

Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO).

Conrad

1996

Microbiological Reviews

1,110

546

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“…Meanwhile, microbes (nutrient reducers) were classified into true, sequential and respirers nitrate reducers [19,13]. Shirey and Sexstone [20] reported Enterobacter sp as one population to reduce nitrate. Many bacteria are capable of producing enzymes that catalyzes the reduction and are capable of utilizing nitrate via assimilatory or dissimilatory pathway and some uses nitrate as final electron acceptor also, reduction may be enhance by organic matter present in a medium containing nitrate [13].…”

Section: Resultsmentioning

confidence: 99%

Bacteria Screening with Sufficient Nitrate Extraction from Wastewater

Bello

Nafi’u

Haruna

et al. 2020

JALSI

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Search for effective biological strain for use to remedy water pollution has been limited. This study was performed to search for effective bacteria capable of extracting nitrate from wastewater. Samples from abattoir inside (Abi) and abattoir outside (Abt), fishpond of kwalkwalawa (Fpk) and mabera fishpond (Fpm) wastewater were used. Using conventional standard plate technique as 1.96×107 cfu/ml (Abi) had highest bacteria count while 1.05×107 cfu/ml (Fpk) had minimal. The obtained pure colonies were morphologically and biochemically characterized including two Enterobacter specie, Proteus and Pseudomonas specie. The isolated organisms were used as a test organism for the removal of nitrate present in high nitrate containing medium. Prior to the extraction, the wastewater samples were physicochemically analyzed using parameters like color, odor, pH, alkalinity, hardness, temperature and nitrate. For nitrate extraction, bacteria were inoculated in a separate tubes containing nitrate broth, incubated in a rotary shaker 150rpm at 37°C for 16hrs. The supernatant from 10minutes centrifuged culture were used for nitrate removal evaluation after a series of reaction and stop using spectrophotometer at 420nm. The absorbance 0.732 proteus specie after estimation of nitrate in a medium was maximal while Enterobacter specie 0.574 was minimal. Meanwhile, two of the Enterobacter specie has similar absorbance 0.012 while Proteus and Pseudomonas species had 0.016 and 0.010 respectively in the nitrate concentration removed within the media. The nitrate present in the medium was compared with a known standard curve prepared with NaNO3 at 100-1000 ppm. The result indicated that bacteria from fish pond wastewaters had highest potential for extracting nitrate and that inherent bacteria are capable of removing pollutant nitrate and the bacteria may be able to remove nitrate efficiently if genetically engineered.

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Denitrification and nitrate-reducing bacterial populations in abandoned and reclaimed minesoils

Cited by 22 publications

References 37 publications

Influence of maize mucilage on the diversity and activity of the denitrifying community

Influence of maize mucilage on the diversity and activity of the denitrifying community

Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO).

Bacteria Screening with Sufficient Nitrate Extraction from Wastewater

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