Land application of poultry litter (PL) presents an opportunity to improve soil productivity and disposal of poultry waste. We investigated methane (CH 4 ) and nitrous oxide (N 2 O) emissions from agricultural soil receiving PL and ammonium nitrate (AN) fertilizers using surface (SA), soil incorporation (SI), and subsurface band (BA) application methods in conventional (CT) and notillage (NT) systems on a Decatur silt loam soil in North Alabama. Plots under CT and NT were sinks of CH 4 in spring, summer, and fall. In winter, the plots had net emissions of 3.32 and 4.24 g CH 4 ha −1 day −1 in CT and NT systems, respectively. Plots which received AN were net emitters of CH 4 and N 2 O, whereas plots which received PL were net sinks of CH 4 . Plots which received PL using SA or SI methods were net emitters of N 2 O, whereas under PL using BA application, the plots were net sinks of N 2 O. Our study indicates that using subsurface band application of PL was the most promising environmentally sustainable poultry waste application method for reducing CH 4 and N 2 O emissions from agricultural soil in NT and CT corn production systems on the Decatur soil in north Alabama.