Denitrification by yeasts and occurrence of cytochrome P450nor in Trichosporon cutaneum

Tsuruta, S

doi:10.1016/s0378-1097(98)00427-3

Cited by 24 publications

(44 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…About two decades ago, we showed that many fungi and yeasts (eukaryotes) also exhibit distinct denitrifying activities [4][5][6]. Before our finding, there were many papers reporting that fungi can evolve a small amount (at most 15%) of N 2 O from, in most cases, nitrite, and thus they may exhibit denitrifying activity [7].…”

mentioning

confidence: 72%

Fungal denitrification and nitric oxide reductase cytochrome P450nor

Shoun

Fushinobu

Jiang

et al. 2012

Phil. Trans. R. Soc. B

225

189

View full text Add to dashboard Cite

We have shown that many fungi (eukaryotes) exhibit distinct denitrifying activities, although occurrence of denitrification was previously thought to be restricted to bacteria (prokaryotes), and have characterized the fungal denitrification system. It comprises NirK (copper-containing nitrite reductase) and P450nor (a cytochrome P450 nitric oxide (NO) reductase (Nor)) to reduce nitrite to nitrous oxide (N 2 O). The system is localized in mitochondria functioning during anaerobic respiration. Some fungal systems further contain and use dissimilatory and assimilatory nitrate reductases to denitrify nitrate. Phylogenetic analysis of nirK genes showed that the fungal-denitrifying system has the same ancestor as the bacterial counterpart and suggested a possibility of its proto-mitochondrial origin. By contrast, fungi that have acquired a P450 from bacteria by horizontal transfer of the gene, modulated its function to give a Nor activity replacing the original Nor with P450nor. P450nor receives electrons directly from nicotinamide adenine dinucleotide to reduce NO to N 2 O. The mechanism of this unprecedented electron transfer has been extensively studied and thoroughly elucidated. Fungal denitrification is often accompanied by a unique phenomenon, co-denitrification, in which a hybrid N 2 or N 2 O species is formed upon the combination of nitrogen atoms of nitrite with a nitrogen donor (amines and imines). Possible involvement of NirK and P450nor is suggested.

show abstract

mentioning

confidence: 72%

Fungal denitrification and nitric oxide reductase cytochrome P450nor

Shoun

Fushinobu

Jiang

et al. 2012

Phil. Trans. R. Soc. B

225

189

View full text Add to dashboard Cite

show abstract

“…However, the role of Cryptococcus in soil has not been clarified yet. With respect to nitrous oxide-producing activity in Cryptococcus strains of Basidiomycota, Cryptococcus gastricus JCM 3691 in the clade Filobasidiales did not produce nitrous oxide or nitric oxide in pure cultures (36). The isolation of Cryptococcus strains from the NR soil is now in progress in our laboratory.…”

Section: Discussionmentioning

confidence: 93%

“…Chemical inhibitors of fungi and bacteria decreased the flux of nitrous oxide by 89% and 23%, respectively, suggesting that the fungal community was involved in the nitrous oxide production. Prior to this finding, experiments in vivo had shown that nitrous oxide was the main end product of fungal denitrification (30,36). Recently, Zhaorigetu et al (40) reported a positive relationship between fungal biomass and nitrous oxide emissions in an upland rice field.…”

mentioning

confidence: 96%

Molecular Characterization of Fungal Communities in Non-Tilled, Cover-Cropped Upland Rice Field Soils

et al. 2010

View full text Add to dashboard Cite

This study aimed to characterize soil fungal communities in upland rice fields managed with tillage/non-tillage and winter cover-cropping (hairy vetch and cereal rye) practices, using PCR-based molecular methods. The study plots were maintained as upland fields for 5 years and the soils sampled in the second and fifth years were analyzed using T-RFLP (terminal restriction fragment length polymorphism) profiling and clone libraries with the internal transcribed spacer (ITS) region and domain 1 (D1) of the fungal large-subunit (fLSU) rRNA (D1fLSU) as the target DNA sequence. From the 2nd-year-sample, 372 cloned sequences of fungal ITS-D1fLSU were obtained and clustered into 80 nonredundant fungal OTUs (operational taxonomic units) in 4 fungal phyla. The T-RFLP profiling was performed with the 2nd-and 5th-year-samples and the major T-RFs (terminal restriction fragments) were identified using a theoretical fragment analysis of the ITS-D1fLSU clones. These molecular analyses showed that the fungal community was influenced more strongly by the cover-cropping than tillage practices. Moreover, the non-tilled, cover-cropped soil was characterized by a predominance of Cryptococcus sp. in the phylum Basidiomycota. We provided a genetic database of the fungal ITS-D1fLSUs in the differently managed soils of upland rice fields.

show abstract

“…Filamentous soil fungi of the genus Fusarium are known to utilise such waste materials as carbon source, and are also known for their significant denitrification rates (Guest and Smith, 2002;Shoun et al, 1992). Since the first empirical evidence was obtained on fungal denitrification, numerous biochemical and molecular studies were conducted on this phenomenon, using pure cultures of various fungal taxa obtained from culture collections (Shoun and Tanimoto 1991;Shoun et al, 1992;Kobayashi et al, 1996;Shoun et al, 1998;Tsuruta et al, 1998;Zhou et al, 2002;Kumon et al, 2002;Watsuji et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Biological nitrate removal from synthetic wastewater using a fungal consortium in one stage bioreactors

Greben¹,

Joubert²,

Tjatji³

et al. 2009

WSA

View full text Add to dashboard Cite

A series of lignocellulosic fungi, capable of cellulase and/or xylanase production, were isolated from soil to be used for cellulose degradation and nitrate removal from nitrate-rich wastewater in simple one-stage anaerobic bioreactors containing grass cuttings as source of cellulose. The fungal consortium, consisting of six hyphomycetous isolates, some of which belong to the genera Fusarium, Mucor and Penicillium, was able to remove a significant portion of the nitrate from the treated water. The results were obtained for three bioreactors, i.e. FR, FRp and AFRp, differing in volume and mode of grass addition. Bioreactor AFRp received autoclaved grass, instead of non-autoclaved grass containing natural microbial consortia, as supplied to FR and FRp. Nitrate removal in FR amounted to 89% removal efficiency, while this was 65% and 67% in FRp and AFRp, respectively. The residual chemical oxygen demand (COD) concentration in FR was higher than 600 mg/ℓ, while it was 355 and 379 mg/ℓ in FRp and AFRp, respectively. The similar nitrate removal results for AFRp and FRp indicated that the micro-organisms attached to grass cuttings did not seem to affect the nitrate removal in the reactor. This observation has led to the conclusion that the fungal consortium was, except for being able to degrade cellulose within the grass cuttings, also responsible for nitrate removal from the synthetic nitrate-rich wastewater.

show abstract

Denitrification by yeasts and occurrence of cytochrome P450nor in Trichosporon cutaneum

Cited by 24 publications

References 10 publications

Fungal denitrification and nitric oxide reductase cytochrome P450nor

Fungal denitrification and nitric oxide reductase cytochrome P450nor

Molecular Characterization of Fungal Communities in Non-Tilled, Cover-Cropped Upland Rice Field Soils

Biological nitrate removal from synthetic wastewater using a fungal consortium in one stage bioreactors

Contact Info

Product

Resources

About