Denitrification kinetics and denitrifier abundances in sediments of lakes receiving atmospheric nitrogen deposition (Colorado, USA)

McCrackin, Michelle L.; Elser, James J.

doi:10.1007/s10533-011-9571-5

Cited by 27 publications

(20 citation statements)

References 75 publications

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“…Overall, these results highlighted that the enhanced DNT rates observed at ecosystem level in the presence of Isoetids (up to 7.7 times increase in vegetated sediments over non‐vegetated sediments) should be taken into account for more accurate modeling N‐processing in lakes. Small water bodies are abundant and more active than large aquatic systems worldwide (Downing et al ), and contribute disproportionately to global carbon budgets (Cole et al ) and to global N losses (Mccrackin and Elser ). The different regulation mechanisms arising from this study (high lability of organic matter potentially inhibiting AOA in sediments colonized by ISO species, and

{NO}_{3}^{-}

as a limiting factor for DNT in sediments colonized by elodeids) may be useful information to enhance DNT in aquatic systems and to ameliorate water quality status of lakes, ponds and pools.…”

Section: Discussionmentioning

confidence: 99%

Macrophyte landscape modulates lake ecosystem-level nitrogen losses through tightly coupled plant-microbe interactions

et al. 2015

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Root functional diversity of submerged vegetation exerts a major effect on nitrogen (N) cycling in lake sediments. This fact, however, is neglected in current N-balance models because the links between the engineering role of plants and in situ microbial N cycling are poorly understood. We hypothesized that macrophyte species with high root oxygen loss (ROL) capacity promote the highest denitrification because of a higher abundance of ammonia oxidizers and tighter coupling between nitrifiers and denitrifier communities. We sampled five small ultraoligotrophic shallow lakes with abundant macrophyte cover including sediments dominated either by Isoetes spp. (high ROL), mixed communities of natopotamids (low ROL), and unvegetated sandy sediments. At each site, we quantified denitrification (DNT) rates and proxies for the abundance of denitrifiers (nirS and nirK genes), and both ammonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) and the diversity of nirS-harboring bacteria. Vegetated sediments showed significantly higher abundances of N-cycling genes than bare sediments. Plant communities dominated by Isoetes generated sediments with higher redox and NO 2 3 concentrations and significantly higher DNT rates than natopotamidsdominated landscapes. Accordingly, increasing DNT rates were observed along the gradient from low ROL plants-bare sediments-high ROL plants. Significantly higher abundance of the archaeal amoA gene was recorded in sediments colonized by high ROL plants unveiling a key biogeochemical role for AOA in coupling macrophyte landscape and ecosystem denitrification.The global nitrogen cycle has been strongly modified by massive industrial fixation of nitrogen gas (N 2 ) for human use and by fossil-fuel combustions (Gruber and Galloway 2008). Current concentrations of bio-available nitrogen (N) forms are higher than ever in the human era (Fowler et al. 2013). Terrestrial and aquatic denitrification (DNT) and anammox processes are of special interest because they represent the only permanent removal pathway whereby bioavailable N is returned to inert N 2 gas (Rockstr€ om et al. 2009). The presence and specific composition of rooted plants is a major factor influencing DNT rates of soils and sediments (RisgaardPetersen and Jensen 1997). Plants can alter physicochemical factors known to control denitrification rates such as pH, oxygen, carbon sources and nitrate concentrations (Griffiths et al. 1997;Gacia et al. 2009). These modifications, in turn, influence the activity, diversity and abundance of rhizosphere nitrifiers and denitrifiers populations although few studies have reported quantitative evidences of these plantmicrobial interactions in lake ecosystems (Kofoed et al. 2012). Commonly, nitrification (NT) produces NO 2 3 under oxic conditions after ammonification while DNT requires suboxic conditions and is highly dependent on both NO 2 3 transport from aerobic to anaerobic zones and changes of the in situ redox conditions (Seitzinger et al. 2006).Submersed aquatic plants (SAV)...

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{NO}_{3}^{-}

as a limiting factor for DNT in sediments colonized by elodeids) may be useful information to enhance DNT in aquatic systems and to ameliorate water quality status of lakes, ponds and pools.…”

Section: Discussionmentioning

confidence: 99%

Macrophyte landscape modulates lake ecosystem-level nitrogen losses through tightly coupled plant-microbe interactions

et al. 2015

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“…Nitrate plus nitrite (hereafter: NO 3 − ) concentration was measured on a Lachat Quick Chem 8000 Autoanalyzer. Data on DOC and NO 3 − concentrations for a subset of lakes were previously reported [ McCrackin and Elser , 2011].…”

Section: Methodsmentioning

confidence: 99%

“…Estimated ambient production of CO 2 , CH 4 , and N 2 O by sediments was determined as the accumulation of these gases for incubations that were not amended with acetylene [ García‐ruiz et al , 1998; Rudaz et al , 1999]. The denitrification rate was determined as the production of N 2 O during the incubations amended with acetylene and has been reported elsewhere [ McCrackin and Elser , 2011]. Sediment gas fluxes are reported on the basis of dry sediment mass that was converted to an areal basis using the sediment bulk density for each lake [ Richardson et al , 2004].…”

Section: Methodsmentioning

confidence: 99%

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Greenhouse gas dynamics in lakes receiving atmospheric nitrogen deposition

McCrackin

Elser

2011

Global Biogeochem. Cycles

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[1] Anthropogenic nitrogen (N) inputs have been found to influence emissions of greenhouse gases from a variety of ecosystems; however, the effects of N loading on greenhouse gas dynamics in lakes are not well documented. We measured concentrations of carbon dioxide (CO 2 ), methane (CH 4 −1 in high-deposition lakes compared to 22 nmol L −1 in low-deposition lakes. Nitrous oxide is of particular interest because it is more potent than CO 2 as a greenhouse gas and because of its role in the destruction of stratospheric ozone. To understand the potential magnitude of lake N 2 O production related to atmospheric N deposition, we applied two published methodologies for determining emissions from aquatic ecosystems to available data sets. We estimated contemporary global N 2 O emissions from lakes to be 0.04 -2 Tg N y −1 , increasing to 0.1 -3.4 Tg N y −1 in 2050. The contemporary estimates represent 13-95% of emissions from rivers and estuaries, suggesting that further research is required to better quantify emission rates from lentic ecosystems.Citation: McCrackin, M. L., and J. J. Elser (2011), Greenhouse gas dynamics in lakes receiving atmospheric nitrogen deposition, Global Biogeochem. Cycles, 25, GB4005,

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“…The variable results in single lake studies, therefore, reflect interlake differences in the dominance of Dw and Dn in denitrification. In contrast, interlake studies undertaken in Norway (Kelly et al 1987, McCrackin & Elser 2010 and the USA (Kelly et al 1987, McCrackin & Elser 2012 indicate that in general, nitrate concentration in the water above the sediment correlates with the sediment denitrification rate and could thus be used as an easily measurable predictor of lacustrine N 2 production at the interlake scale. These studies, however, were conducted using either the acetylene inhibition technique (McCrackin & Elser 2012) or 15 N-labelling (Kelly et al 1987), which do not account for Dn (e.g.…”

Section: Introductionmentioning

confidence: 96%

Interlake variation and environmental controls of denitrification across different geographical scales

Rissanen¹,

Tiirola²,

Hietanen³

et al. 2013

Aquat. Microb. Ecol.

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Denitrification in lakes significantly reduces the nitrogen (N) load from land to oceans, but the factors controlling it remain poorly understood. Therefore, interlake variation of denitrification in sediments of small to medium-sized lakes (from 0.03 to 17.8 km 2 ) was studied across different geographical scales. At the local scale, the denitrification rates and sediment microbial communities were studied in 4 boreal lakes in close proximity (within 20 km) using the isotope pairing technique (IPT) and molecular methods. These local scale data were combined with previously published data on denitrification rates from 10 other European lakes to extend the analysis to the regional (boreal area) and continental (boreal and temperate areas) scales. Denitrification varied considerably among lakes, ranging from ~50 to ~600, ~0 to ~600 and ~0 to ~12900 µmol N m −2 d −1at the local, regional and continental scales, respectively. This variation was primarily due to nitrate availability. The structure of the denitrifier community studied at the local scale was independent of the denitrification rates but varied among lakes correlating with nitrate availability and sediment organic content. Removal of nitrate and total N load by denitrification was less efficient in boreal than in temperate lakes. In addition, a meta-analysis of published N mass balance data indicates that the total N retention (denitrification + N sedimentation) is less efficient in boreal than in temperate lakes. Anaerobic ammonium oxidation (anammox) was studied at the local scale but was not detected, although (based on molecular markers) several anammox genera were present in the sediments.KEY WORDS: Denitrification · Lake · Sediment · nirK Resale or republication not permitted without written consent of the publisherRemoval of nitrate and total N load by denitrification was less efficient in boreal than in temperate lakes. Photos: Ilpo Hakala (boreal) and Thyes (temperate)Aquat Microb Ecol 69: [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] 2013

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Denitrification kinetics and denitrifier abundances in sediments of lakes receiving atmospheric nitrogen deposition (Colorado, USA)

Cited by 27 publications

References 75 publications

Macrophyte landscape modulates lake ecosystem-level nitrogen losses through tightly coupled plant-microbe interactions

Macrophyte landscape modulates lake ecosystem-level nitrogen losses through tightly coupled plant-microbe interactions

Greenhouse gas dynamics in lakes receiving atmospheric nitrogen deposition

Interlake variation and environmental controls of denitrification across different geographical scales

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