Denitrification of industrial wastewater: Influence of glycerol addition on metabolic activity and community shifts in a microbial consortium

Cyplik, Paweł; Juzwa, Wojciech; Marecik, Roman; Powierska-Czarny, Jolanta; Piotrowska-Cyplik, Agnieszka; Czarny, Jakub; Drożdżyńska, Agnieszka; Chrzanowski, Łukasz

doi:10.1016/j.chemosphere.2013.09.083

“…To determine the DN phylotypes present in the DN consortia, 16S amplicon sequencing was performed, and it was observed that at the species level P. tropicalis and P. aeruginosa for Halo and C. werkmanii and C. freundii for U-166 were the most dominant and are known denitrifiers 9, 10 . It was also observed that the Citrobacter dominating the U-166 DN consortia consumed nitrate at a rate that was faster than other industrial microbial consortia containing Citrobacter adapted for denitrification 11 .…”

Section: Discussionmentioning

confidence: 96%

Laboratory growth of denitrifying water column microbial consortia from deep-sea shipwrecks in the northern Gulf of Mexico

Haridas¹,

Biffinger²,

Boyd³

et al. 2018

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Background: Shipwrecks serve as a rich source for novel microbial populations that have largely remained undiscovered. Low temperatures, lack of sunlight, and the availability of substrates derived from the shipwreck’s hull and cargo may provide an environment in which microbes can develop unique metabolic adaptations. Methods: To test our hypothesis that shipwrecks could influence the microbial population involved in denitrification when a consortium is grown in the laboratory, we collected samples proximate to two steel shipwrecks in the northern Gulf of Mexico. Then under laboratory conditions, we grew two independent denitrifying microbial consortia. Each consortium was grown by using the BART assay system and analyzed based on growth kinetics, ion chromatography and 16S amplicon sequencing. Results: Both denitrifying consortia were different from each other based on varied growth profiles, rates of nitrate utilization and 16S amplicon sequencing. Conclusions: Our observations conclude that the laboratory grown water column microbial consortia from deep-sea shipwrecks in the Gulf of Mexico are able to undergo aggressive denitrification.

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“…To determine the DN phylotypes present in the DN consortia, 16S amplicon sequencing was performed, and it was observed that at the species level P. tropicalis and P. aeruginosa for Halo and C. werkmanii and C. freundii for U-166 were the most dominant and are known denitrifiers 9 , 10 . It was also observed that the Citrobacter dominating the U-166 DN consortia consumed nitrate at a rate that was faster than other industrial microbial consortia containing Citrobacter adapted for denitrification 11 .…”

Section: Discussionmentioning

confidence: 96%

Laboratory growth of denitrifying water column microbial consortia from deep-sea shipwrecks in the northern Gulf of Mexico

Haridas

¹

,

Biffinger

²

,

Boyd

³

et al. 2018

View full text Add to dashboard Cite

Background: Shipwrecks serve as a rich source for novel microbial populations that have largely remained undiscovered. Low temperatures, lack of sunlight, and the availability of substrates derived from the shipwreck’s hull and cargo may provide an environment in which microbes can develop unique metabolic adaptations. Methods: To test our hypothesis that shipwrecks could influence the microbial population involved in denitrification when a consortium is grown in the laboratory, we collected samples proximate to two steel shipwrecks in the northern Gulf of Mexico. Then under laboratory conditions, we grew two independent denitrifying microbial consortia. Each consortium was grown by using the BART assay system and analyzed based on growth kinetics, ion chromatography and 16S amplicon sequencing. Results: Both denitrifying consortia were different from each other based on varied growth profiles, rates of nitrate utilization and 16S amplicon sequencing. Conclusions: Our observations conclude that the laboratory grown water column microbial consortia from deep-sea shipwrecks in the Gulf of Mexico are able to undergo aggressive denitrification.

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“…The analysis of metabolic activity was based on the procedure described by Cyplik et al (2013) [42]. Approximately 1 mL of the culture medium was taken for analysis after 24 and 168 h of incubation.…”

Section: Analysis Of Metabolic Activitymentioning

confidence: 99%

Heavy Metals as a Factor Increasing the Functional Genetic Potential of Bacterial Community for Polycyclic Aromatic Hydrocarbon Biodegradation

Staninska-Pięta

¹

,

Czarny²,

Piotrowska-Cyplik

³

et al. 2020

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The bioremediation of areas contaminated with hydrocarbon compounds and heavy metals is challenging due to the synergistic toxic effects of these contaminants. On the other hand, the phenomenon of the induction of microbial secretion of exopolysaccharides (EPS) under the influence of heavy metals may contribute to affect the interaction between hydrophobic hydrocarbons and microbial cells, thus increasing the bioavailability of hydrophobic organic pollutants. The purpose of this study was to analyze the impact of heavy metals on the changes in the metapopulation structure of an environmental consortium, with particular emphasis on the number of copies of orthologous genes involved in exopolysaccharide synthesis pathways and the biodegradation of hydrocarbons. The results of the experiment confirmed that the presence of heavy metals at concentrations of 50 mg·L−1 and 150 mg·L−1 resulted in a decrease in the metabolic activity of the microbial consortium and its biodiversity. Despite this, an increase in the biological degradation rate of polycyclic aromatic hydrocarbons was noted of 17.9% and 16.9%, respectively. An assessment of the estimated number of genes crucial for EPS synthesis and biodegradation of polycyclic aromatic hydrocarbons confirmed the relationship between the activation of EPS synthesis pathways and polyaromatic hydrocarbon biodegradation pathways. It was established that microorganisms that belong to the Burkholderiales order are characterized by a high representation of the analyzed orthologs and high application potential in areas contaminated with heavy metals and hydrocarbons.

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Denitrification of industrial wastewater: Influence of glycerol addition on metabolic activity and community shifts in a microbial consortium

Cited by 27 publications

References 47 publications

Laboratory growth of denitrifying water column microbial consortia from deep-sea shipwrecks in the northern Gulf of Mexico

Laboratory growth of denitrifying water column microbial consortia from deep-sea shipwrecks in the northern Gulf of Mexico

Laboratory growth of denitrifying water column microbial consortia from deep-sea shipwrecks in the northern Gulf of Mexico

Heavy Metals as a Factor Increasing the Functional Genetic Potential of Bacterial Community for Polycyclic Aromatic Hydrocarbon Biodegradation

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