Dense high aspect ratio nanostructures for cell chip applications - Fabrication, replication, and cell interactions

Pribyl, Markus; Taus, Philipp; Prado‐Lopez, Sonia; Dozio, Samuele M.; Schrenk, W.; Haslinger, Michael J.; Kopp, Sonja; Mühlberger, M.; Wanzenboeck, Heinz D.

doi:10.1016/j.mne.2022.100121

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Our fabrication method is based on nanoimprint lithography (NIL) (e.g., [21][22][23][24][25]), since it is a scalable, mass-fabrication-suited method for the cost-efficient replication of micro-and nanostructures. NIL has been shown to be able to provide complex micro-and nanotopographies in a single processing step (e.g., [26][27][28][29]), as well as the direct structuring of biocompatible materials (e.g., [30][31][32][33][34]). For nanoimprinting, a master structure is always necessary, and from which a stamp is typically replicated, which contains the negative features of the original master structure.…”

Section: Introductionmentioning

confidence: 99%

Nanoimprinted Hierarchical Micro-/Nanostructured Substrates for the Growth of Cardiomyocyte Fibers

Mühlberger,

Kopp,

Deyett

et al. 2023

Nanomanufacturing

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Investigating the behavior of cardiomyocytes is an important part of drug development. We present a structure and a related nanoimprint-based fabrication method, where the cardiomyocytes form isolated fibers, which is beneficial for drug testing, more closely representing the structure of the cardiomyocytes in vivo. We found that channel structures with walls with a rough top surface stimulate cardiomyocytes to form such fibers, as desired. Nanoimprint lithography is used as a fast and cost-efficient method to fabricate our hierarchically structured cell growth substrates.

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Section: Introductionmentioning

confidence: 99%

Nanoimprinted Hierarchical Micro-/Nanostructured Substrates for the Growth of Cardiomyocyte Fibers

Mühlberger,

Kopp,

Deyett

et al. 2023

Nanomanufacturing

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“…In particular, when it comes to anti-reflection and bactericidal effects, HAR nanostructures, which are nanostructures with a large length-to-diameter ratio, are of great interest [3][4][5]. In technical applications, HAR structures of various sizes are utilized for bactericidal surfaces [6], broadband anti-reflective surfaces [7,8], the modification of stem cells [9,10], in microfluidics [9,11,12], as microneedles [13] or in nanogenerators [14]. In life science applications, well-defined and isolated nanopillars (sometimes called nanoneedles, nanoneedle electrodes or nanoelectrodes if electrically conductive) are of importance, for example, to penetrate cell membranes [15], for intracellular applications or to make recordings [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Increasing the Stability of Isolated and Dense High-Aspect-Ratio Nanopillars Fabricated Using UV-Nanoimprint Lithography

et al. 2023

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Structural anti-reflective coating and bactericidal surfaces, as well as many other effects, rely on high-aspect-ratio (HAR) micro- and nanostructures, and thus, are of great interest for a wide range of applications. To date, there is no widespread fabrication of dense or isolated HAR nanopillars based on UV nanoimprint lithography (UV-NIL). In addition, little research on fabricating isolated HAR nanopillars via UV-NIL exists. In this work, we investigated the mastering and replication of HAR nanopillars with the smallest possible diameters for dense and isolated arrangements. For this purpose, a UV-based nanoimprint lithography process was developed. Stability investigations with capillary forces were performed and compared with simulations. Finally, strategies were developed in order to increase the stability of imprinted nanopillars or to convert them into nanoelectrodes. We present UV-NIL replication of pillars with aspect ratios reaching up to 15 with tip diameters down to 35 nm for the first time. We show that the stability could be increased by a factor of 58 when coating them with a 20 nm gold layer and by a factor of 164 when adding an additional 20 nm thick layer of SiN. The coating of the imprints significantly improved the stability of the nanopillars, thus making them interesting for a wide range of applications.

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The Role of Silicon Technology in Organ‐On‐Chip: Current Status and Future Perspective

Skottvoll,

Escobedo‐Cousin,

Mielnik

2024

Adv Materials Technologies

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Organ‐on‐chip (OoC) systems are microfluidic in vitro platforms constructed to expand the current understanding of organ‐level physiology and response. This technology holds significant potential to transform drug discovery, precision medicine, and disease modeling while reducing animal model use. Recent developments in OoC technology have shown great promise, demonstrated using relatively simple microfluidic designs. Currently, the consensus in the OoC‐related literature is that the future of OoC technology lies in the development of robust platforms that offer higher throughput, improved customization, and higher levels of integration of sensing and actuation modalities. The implementation of silicon micro‐nanofabrication technologies can foster such a transition, but the application in the field remains limited. In this review, an overview of silicon micro‐nanofabrication technologies is provided that have been or can be applied in the realization of compact OoC systems, with focus on integrated actuation and sensing modalities. Emerging technologies are highlighted for the heterogeneous integration of silicon‐based and polymer‐based components in OoC systems for the realization of compact and multimodal OoC platforms. Finally, the most promising avenues are outlined for silicon technology within the framework of OoC and the future of biomedical research and personalized medicine.

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Dense high aspect ratio nanostructures for cell chip applications - Fabrication, replication, and cell interactions

Cited by 3 publications

References 30 publications

Nanoimprinted Hierarchical Micro-/Nanostructured Substrates for the Growth of Cardiomyocyte Fibers

Nanoimprinted Hierarchical Micro-/Nanostructured Substrates for the Growth of Cardiomyocyte Fibers

Increasing the Stability of Isolated and Dense High-Aspect-Ratio Nanopillars Fabricated Using UV-Nanoimprint Lithography

The Role of Silicon Technology in Organ‐On‐Chip: Current Status and Future Perspective

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