Dense optical flow software to quantify cellular contractility

Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes

“…To find the best rate, note that the minimum acquisition period to reliably reconstruct a signal needs to be smaller than half the time of the fastest component ( Nyquist, 1928 ). For more details see Scalzo et al. (2021) (User Manual provided as Methods S1, section 2.2 Requirements, subsection: Minimum frame rate).…”

“…Once high-quality data is acquired, the analysis of contractility parameters can be performed using a variety of available software programs (IonWizard Ionoptix; Boudaoud et al., 2014 ; Grune et al., 2019 ; Maddah et al., 2015 ; Pasqualin et al., 2016 ; Sala et al., 2018 ). The authors have developed CONTRACTIONWAVE ( Scalzo et al., 2021 ), a high-performance Python program that processes video micrograph films using dense optical flow image analysis. This program has been validated for large-scale analysis of different cell types (adult-, neonatal- and hiPSC-CMs), treated or not with drugs, including both healthy cells and cells isolated from animal models of human disease.…”

“…Colored circles presented in the contraction-relaxation wave (bottom panel) represent (1) contraction initiation; (2) maximum contraction speed (MCS); (3) minimum contraction speed; (4) maximum relaxation speed (MRS); (5) return to baseline. Figure adapted from Scalzo et al. (2021) …”

“…Moreover, we use CONTRACTIONWAVE software to analyze and show the optimized results. For complete details on the use and execution of this profile, please refer to Scalzo et al. (2021) .…”

“…For complete details on the use and execution of this profile, please refer to Scalzo et al. (2021) .…”