Dense, porous, and fibrous scaffolds composed of PHBV, PCL, and their 75:25 blend: an <i>in vitro</i> morphological and cytochemical characterization

Modolo, Larissa Pereira; França, Wellington Raimundo; Simbara, Márcia Mayumi Omi; Malmonge, Sônia Maria; Santos, Arnaldo Rodrigues

doi:10.1080/1023666x.2022.2148409

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…Dichloromethane contributes to this morphology as it has a low boiling point and high volatility Modolo et al (2023). state that porous and fibrillar structures facilitate vascularization, nutrient diffusion, adhesion, and cell growth[35]. Similar results can be found in the work ofAltun et al (2022) [36].…”

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confidence: 59%

“…Several studies have demonstrated that the choice of solvent directly influences the structure of materials obtained by the rotary jet spinning technique[34]. Dichloromethane contributes to this morphology as it has a low boiling point and high volatility Modolo et al (2023). state that porous and fibrillar structures facilitate vascularization, nutrient diffusion, adhesion, and cell growth[35].…”

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confidence: 99%

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Poly (ε-caprolactone)-Based Scaffolds with Multizonal Architecture: Synthesis, Characterization, and In Vitro Tests

Lima,

Canelas,

Dutra

et al. 2023

Polymers

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Tissue engineering is vital in treating injuries and restoring damaged tissues, aiming to accelerate regeneration and optimize the complex healing process. In this study, multizonal scaffolds, designed to mimic tissues with bilayer architecture, were prepared using the rotary jet spinning technique (RJS scaffolds). Polycaprolactone and different concentrations of alginate hydrogel (2, 4, and 6% m/v) were used. The materials were swollen in pracaxi vegetable oil (PO) (Pentaclethra macroloba) and evaluated in terms of surface morphology, wettability, functional groups, thermal behavior, crystallinity, and cytotoxicity. X-ray diffraction (XRD) showed the disappearance of the diffraction peak 2θ = 31.5° for samples from the polycaprolactone/pracaxi/alginate (PCLOA) group, suggesting a reduction of crystallinity according to the presence of PO and semi-crystalline structure. Wettability gradients (0 to 80.91°) were observed according to the deposition layer and hydrogel content. Pore diameters varied between 9.27 μm and 37.57 μm. Molecular interactions with the constituents of the formulation were observed via infrared spectra with Fourier transform (FTIR), and their influence was detected in the reduction of the maximum degradation temperature within the groups of scaffolds (polycaprolactone/alginate (PCLA) and PCLOA) about the control. In vitro tests indicated reduced cell viability in the presence of alginate hydrogel and PO, respectively.

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confidence: 59%

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confidence: 99%

Poly (ε-caprolactone)-Based Scaffolds with Multizonal Architecture: Synthesis, Characterization, and In Vitro Tests

Lima,

Canelas,

Dutra

et al. 2023

Polymers

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“…MicroCT showed that the cells were capable of infiltrating the fibrous mesh of the materials studied, growing in more than one plane and on the surface of the materials. Recent data indicate that cells are active and viable for at least 120 h on fibrous scaffolds like the ones described here 27 . Empirically, the metabolism of the culture medium also indicates cell viability inside the meshes.…”

Section: Discussionmentioning

confidence: 55%

“…Recent data indicate that cells are active and viable for at least 120 h on fibrous scaffolds like the ones described here. 27 Empirically, the metabolism of the culture medium also indicates cell viability inside the meshes. However, an in-depth study of the behavior and cell viability inside the meshes is still needed.…”

Section: Discussionmentioning

confidence: 99%

Morphological and biochemical analysis of cells cultured on fibrous poly(ε‐caprolactone) scaffolds with different degrees of fiber alignment produced by solution blow spinning

et al. 2023

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BackgroundBioresorbable materials are compounds that decompose in physiological mediums both in vitro and in vivo and are used as an alternative to temporary implants in injured tissues. The aim of this study was to analyze the morphology and cytochemistry of cells grown on fibrous poly(ε‐caprolactone) (PCL) scaffolds and to measure cell metabolism parameters by biochemical analysis of the conditioned culture medium from cells grown on the scaffolds.MethodsFibrous PCL scaffolds were used under the following conditions: unaligned fibers (NA), fibers aligned at 150 rpm (A150), and fibers aligned at 300 rpm (A300). Vero cells were cultured on these scaffolds for 24 h, 48 h, and 72 h. Samples were analyzed by SEM, MicroCT, cytochemistry, and culture medium biochemistry.ResultsThe results of the cytochemical analysis showed cells were confluent and well spread on the culture plate, while cells grown on the polymeric scaffold, exhibited an elongated morphology. In the biochemical analyses, no significant differences were observed in the expression of alkaline phosphatase or in the levels of cholesterol or total protein in the culture medium. The different materials do not seem to promote changes in the expression or metabolism of these molecules. Only glucose was markedly reduced in the culture medium of cells grown on either aligned or unaligned scaffolds for 48 h or 72 h. This finding indicates the intense energy requirements of cells grown on these scaffolds.ConclusionPCL fibers showed a great capacity to support cell growth. These data reinforce the interpretation that cells grow satisfactorily on PCL scaffolds.

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“…Blending has been reported as an effective way to improve and tailor the properties of PHBV to certain applications. Common polymers for this strategy include poly(lactic acid) (PLA) [14,15], poly(ethylene succinate) (PES) [16], poly(butylene-co-succinate-coadipate) (PBSA) [17,18], poly(caprolactone) (PCL) [19,20], polyurethane (PU) [21,22], and cellulose [23,24]. Moreover, other functional fillers have been evaluated including graphene nanoplatelets [25] or carbon nanotubes [26] as conductive fillers, superparamagnetic iron oxide nanoparticles for biomedical applications [27], and silver as an antibacterial agent [28], among others.…”

Section: Introductionmentioning

confidence: 99%

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blends with Poly(caprolactone) and Poly(lactic acid): A Comparative Study

Tubio,

Valle,

Carvalho

et al. 2023

Polymers

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Poly(hydroxybutyrate-co-hidroxyvalerate) (PHBV) is a biodegradable polymer, which is a potential substitute for plastics made from fossil resources. Due to its practical interest in the field of tissue engineering, packaging, sensors, and electronic devices, the demand for PHBV with specific thermal, electrical, as well as mechanical requirements is growing. In order to improve these properties, we have developed PHBV blends with two thermoplastic biodegradable polyesters, including poly(caprolactone) (PCL) and poly(lactic acid) (PLA). We analysed the effect of these biopolymers on the morphological, wetting, structural, thermal, mechanical, and electrical characteristics of the materials. Further, the biodegradation of the samples in simulated body fluid conditions was evaluated, as well as the antibacterial activity. The results demonstrate that the blending with PCL and PLA leads to films with a dense morphology, increases the hydrophilic character, and induces a reinforcement of the mechanical characteristics with respect to pristine PHBV. In addition, a decrease in dielectric constant and a.c. electrical conductivity was noticed for PHBV/PLA and PHBV/PCL blends compared to neat PHBV polymer. All neat polymers and blends showed antibacterial properties against S. aureus, with more than 40% bacterial reduction, which increased to 72% in the presence of PCL polymer for a blend ratio of 50/50. Thus, it is demonstrated a suitable way to further tailor a variety of functionalities of PHBV for specific applications, by the development of polymer blends with PLA or PCL.

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Dense, porous, and fibrous scaffolds composed of PHBV, PCL, and their 75:25 blend: an in vitro morphological and cytochemical characterization

Cited by 8 publications

References 37 publications

Poly (ε-caprolactone)-Based Scaffolds with Multizonal Architecture: Synthesis, Characterization, and In Vitro Tests

Poly (ε-caprolactone)-Based Scaffolds with Multizonal Architecture: Synthesis, Characterization, and In Vitro Tests

Morphological and biochemical analysis of cells cultured on fibrous poly(ε‐caprolactone) scaffolds with different degrees of fiber alignment produced by solution blow spinning

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blends with Poly(caprolactone) and Poly(lactic acid): A Comparative Study

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